BCH 330 FINAL EXAM NEWEST 2025 TESTING ACTUAL
EXAM WITH 180 QUESTIONS AND CORRECT DETAILED
ANSWERS (VERIFIED ANSWERS) ALREADY GRADED A+
(BRAND NEW) GUARANTEED PASS
A _____ protein known as ____ contains 3 AMINO ACIDS that undergo an
oxidative reaction to form an intense _____ fluorophore. This protein is
remarkably useful because the fluorophore is _____. Importantly, GFP can be
attached to form a ____ with a second protein of interest during standard cloning
procedures. β-barrel, GFP, intrinsic, encoded in the amino acid sequence, fusion
protein
Bacteria move in a ______ mode. They sense that the concentration of a nutrient
is increasing and _____ in one direction. When they do not sense an increasing
gradient, the direction of flagellar rotation reverse. Since the flagellar fibers are
not meant to go in that direction, the bacterium ________. roll-and-tumble
mode, rotate their flagellum, tumbles in a random-walk fashion.
_____ is a support medium that has two functions: 1) prevents molecules from
diffusing into broad bands 2) provides a sieving material that obstructs the
movement of larger particles more than smaller ones, allowing them to separate.
The term _______ refers to a specific gel layer technique that "focuses" a protein
band. The mechanism involves creating a large voltage gradient between Cl- and
the _____ in a later called the _______, which has a reduced pH.
Polyacrylamide, discontinuous gel, glycinate zwitterion, stacking gel
,The following equation is the analytical version of the principle of
______________________
the rate of a chemical reaction is directly proportional to the product of the
activities or concentrations of the reactants
∆G= ∆G₀ + RTlnQ, where Q=[C][D]/[A][B] mass action
What emphasizes the relative contributions of enthalpic and entropic energies to
the stabilization of globular proteins? hydrophobic effect
What does ∆G stand for? standard state free energy
What is calculated by the following equation?
K = [products] / [reactants] equilibrium constant
H = U + PV
Describes the energy absorbed/released during the making/breaking of bonds.
Enthalpy (∆H)
∆H describes the energy associated with ______. bond breakage/dissociation
,The term ∆S refers to _____, which is defined as ______. entropy, number of
available configurations
_______ are dependent only on the total concentration of a solute species.
Examples include pressure lowering, boiling point elevation, freezing point
depression, and osmotic pressure. colligative properties
_______ is the phenomena by which H₂O tries to dilute the counterions that are
bound to the macromolecule. This makes the concentration of H₂O higher near
the molecule than in the bulk solution. This results in the property of _____ in
which a charged protein or nucleic acid contains an electrostricted ion-H₂O shell.
Donan effect, electrostriction
colligative properties may also be related
________ defines the concentration dependence of ∆G. One example is that it
drives osmotic pressure.
μ = ∆G/∆n = ∆G/(∆cV) chemical potenial
________ definitions and their equations are used to describe a derivative when
one or more other variables are held constant partial differentials
∆U = q + w
, where ∆U refers to the _____ of a ______, as contributed to by ______ (q) and
work (w). The amount of energy it takes to go from one state to another.
ΔU = TΔS + uΔn - PΔV internal energy, system, heat
_____ (___) has the dimensions of energy and can be used to calculate ______
using the following equation:
¹/₂ KbT, where Kb = 1.381x10^-23 JK^-1 Boltzman's contant (Kb), degrees of
freedom
Q = Σj cj = Σj exp (-ΔGj/RT) describes _______, which is the ratio of concentrations
of a compound in a mixture of two immiscible phases at equilibrium. This ratio is
therefore a measure of the difference in solubility of the compound in these two
phases.
Example of this is dissolving benzene in H₂O (Csw) vs. oil (Cso) or the difference
between an AA presence in water or benzene to determine its
hydrophobicity/philicity partition coefficient
d ln Keq / d (1/T) = -ΔH°/R
ln Keq = -∆H/RT + ∆S/R
EXAM WITH 180 QUESTIONS AND CORRECT DETAILED
ANSWERS (VERIFIED ANSWERS) ALREADY GRADED A+
(BRAND NEW) GUARANTEED PASS
A _____ protein known as ____ contains 3 AMINO ACIDS that undergo an
oxidative reaction to form an intense _____ fluorophore. This protein is
remarkably useful because the fluorophore is _____. Importantly, GFP can be
attached to form a ____ with a second protein of interest during standard cloning
procedures. β-barrel, GFP, intrinsic, encoded in the amino acid sequence, fusion
protein
Bacteria move in a ______ mode. They sense that the concentration of a nutrient
is increasing and _____ in one direction. When they do not sense an increasing
gradient, the direction of flagellar rotation reverse. Since the flagellar fibers are
not meant to go in that direction, the bacterium ________. roll-and-tumble
mode, rotate their flagellum, tumbles in a random-walk fashion.
_____ is a support medium that has two functions: 1) prevents molecules from
diffusing into broad bands 2) provides a sieving material that obstructs the
movement of larger particles more than smaller ones, allowing them to separate.
The term _______ refers to a specific gel layer technique that "focuses" a protein
band. The mechanism involves creating a large voltage gradient between Cl- and
the _____ in a later called the _______, which has a reduced pH.
Polyacrylamide, discontinuous gel, glycinate zwitterion, stacking gel
,The following equation is the analytical version of the principle of
______________________
the rate of a chemical reaction is directly proportional to the product of the
activities or concentrations of the reactants
∆G= ∆G₀ + RTlnQ, where Q=[C][D]/[A][B] mass action
What emphasizes the relative contributions of enthalpic and entropic energies to
the stabilization of globular proteins? hydrophobic effect
What does ∆G stand for? standard state free energy
What is calculated by the following equation?
K = [products] / [reactants] equilibrium constant
H = U + PV
Describes the energy absorbed/released during the making/breaking of bonds.
Enthalpy (∆H)
∆H describes the energy associated with ______. bond breakage/dissociation
,The term ∆S refers to _____, which is defined as ______. entropy, number of
available configurations
_______ are dependent only on the total concentration of a solute species.
Examples include pressure lowering, boiling point elevation, freezing point
depression, and osmotic pressure. colligative properties
_______ is the phenomena by which H₂O tries to dilute the counterions that are
bound to the macromolecule. This makes the concentration of H₂O higher near
the molecule than in the bulk solution. This results in the property of _____ in
which a charged protein or nucleic acid contains an electrostricted ion-H₂O shell.
Donan effect, electrostriction
colligative properties may also be related
________ defines the concentration dependence of ∆G. One example is that it
drives osmotic pressure.
μ = ∆G/∆n = ∆G/(∆cV) chemical potenial
________ definitions and their equations are used to describe a derivative when
one or more other variables are held constant partial differentials
∆U = q + w
, where ∆U refers to the _____ of a ______, as contributed to by ______ (q) and
work (w). The amount of energy it takes to go from one state to another.
ΔU = TΔS + uΔn - PΔV internal energy, system, heat
_____ (___) has the dimensions of energy and can be used to calculate ______
using the following equation:
¹/₂ KbT, where Kb = 1.381x10^-23 JK^-1 Boltzman's contant (Kb), degrees of
freedom
Q = Σj cj = Σj exp (-ΔGj/RT) describes _______, which is the ratio of concentrations
of a compound in a mixture of two immiscible phases at equilibrium. This ratio is
therefore a measure of the difference in solubility of the compound in these two
phases.
Example of this is dissolving benzene in H₂O (Csw) vs. oil (Cso) or the difference
between an AA presence in water or benzene to determine its
hydrophobicity/philicity partition coefficient
d ln Keq / d (1/T) = -ΔH°/R
ln Keq = -∆H/RT + ∆S/R
