BIO 242 [information list]
question and answers
asess TWO.
What kind of information should you write down in your physical notebook? - ANSWERS-Time,
temperature, volume, different order of the reagent added.
Lab 1 - ANSWERS-PCR- Based detection of genetically modified foods
Lab 2 - ANSWERS-forensic DNA-fingerprinting : variable number repeats (VNTR) at the D1S80
locus
Lab 3 - ANSWERS-Which kind of coffee drinker are you? Exploring genetic variation in caffeine
metabolism gene.
Lab 4 - ANSWERS-Lactose intolerance (SNP)
µL - ANSWERS-for transferring or measuring small quantities
P1000 (35%) - ANSWERS-100-1000 microliters,
350 - 1000
,P200 (35%) - ANSWERS-20-200 microliters
70-200 microliters
P20 (35%) - ANSWERS-2-20 microliters
7-20 microliters
P10 used by - ANSWERS-TA or TI
Operating range for most pipettes is -% to -% of the indicated volume - ANSWERS-10-100%
Below --% is highly technique dependent - ANSWERS-35
You can still use less than the 35% volume and up when pipetting but it will.... - ANSWERS-
increase the percent error
Tip immersion: ----- before blow out with ------ liquids. If dispensing directing into the liquid keep
piston at the ----- stop until the tip is removed to prevent unintentional aspiration. - ANSWERS-
Pause, viscous, second
Touching off - ANSWERS-slide the tip along the side of the container while pulling up to ensue
all the liquid has been expelled
Tip immersion angle - ANSWERS-should be as close to vertical as possible... not greater than 20˚
If the tip is immersed too deeply - ANSWERS-liquids will rise into the tip because of air pressure
(you could aspirate more sample then intended).
, If the tip is too shallow - ANSWERS-it could result in sample splash up events.
DNA isolation - ANSWERS-aims to collect as much DNA as possible
DNA purification - ANSWERS-aims to reduce contamination from collected DNA
DNA extraction - ANSWERS-dna purifikation and isolation
Chemical DNA extraction methods can be both ------- and --------. - ANSWERS-organic and
inorganic
Organic methods: - ANSWERS-Phenol-cholorform
Inorganic method: - ANSWERS-Proteiniase K, Salting out, silica gel based
Physical DNA extraction methods: - ANSWERS-magnetic bead, paper DNA extraction
Magnetic bead DNA extraction method: - ANSWERS-the DNA is operated under a magnetic field
using positively charged magnetic beads
Paper DNA extraction method: - ANSWERS-anion exchange chromatography; using positively
charged resins (e.g Chelex)
Proteinase K DNA extraction method: - ANSWERS-digest proteins
Salting out DNA extraction method: - ANSWERS-use of salts ( sodium chloride, potassium
acetate and ammonium acetate)
question and answers
asess TWO.
What kind of information should you write down in your physical notebook? - ANSWERS-Time,
temperature, volume, different order of the reagent added.
Lab 1 - ANSWERS-PCR- Based detection of genetically modified foods
Lab 2 - ANSWERS-forensic DNA-fingerprinting : variable number repeats (VNTR) at the D1S80
locus
Lab 3 - ANSWERS-Which kind of coffee drinker are you? Exploring genetic variation in caffeine
metabolism gene.
Lab 4 - ANSWERS-Lactose intolerance (SNP)
µL - ANSWERS-for transferring or measuring small quantities
P1000 (35%) - ANSWERS-100-1000 microliters,
350 - 1000
,P200 (35%) - ANSWERS-20-200 microliters
70-200 microliters
P20 (35%) - ANSWERS-2-20 microliters
7-20 microliters
P10 used by - ANSWERS-TA or TI
Operating range for most pipettes is -% to -% of the indicated volume - ANSWERS-10-100%
Below --% is highly technique dependent - ANSWERS-35
You can still use less than the 35% volume and up when pipetting but it will.... - ANSWERS-
increase the percent error
Tip immersion: ----- before blow out with ------ liquids. If dispensing directing into the liquid keep
piston at the ----- stop until the tip is removed to prevent unintentional aspiration. - ANSWERS-
Pause, viscous, second
Touching off - ANSWERS-slide the tip along the side of the container while pulling up to ensue
all the liquid has been expelled
Tip immersion angle - ANSWERS-should be as close to vertical as possible... not greater than 20˚
If the tip is immersed too deeply - ANSWERS-liquids will rise into the tip because of air pressure
(you could aspirate more sample then intended).
, If the tip is too shallow - ANSWERS-it could result in sample splash up events.
DNA isolation - ANSWERS-aims to collect as much DNA as possible
DNA purification - ANSWERS-aims to reduce contamination from collected DNA
DNA extraction - ANSWERS-dna purifikation and isolation
Chemical DNA extraction methods can be both ------- and --------. - ANSWERS-organic and
inorganic
Organic methods: - ANSWERS-Phenol-cholorform
Inorganic method: - ANSWERS-Proteiniase K, Salting out, silica gel based
Physical DNA extraction methods: - ANSWERS-magnetic bead, paper DNA extraction
Magnetic bead DNA extraction method: - ANSWERS-the DNA is operated under a magnetic field
using positively charged magnetic beads
Paper DNA extraction method: - ANSWERS-anion exchange chromatography; using positively
charged resins (e.g Chelex)
Proteinase K DNA extraction method: - ANSWERS-digest proteins
Salting out DNA extraction method: - ANSWERS-use of salts ( sodium chloride, potassium
acetate and ammonium acetate)
