150 QUESTIONS AND DETAILED ANSWERS
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1. Introduction to Phlebotomy
Q1: What is the primary role of a phlebotomist?
A1: The primary role of a phlebotomist is to collect blood specimens for laboratory testing,
ensuring accuracy and patient safety during the process.
Q2: What is informed consent in phlebotomy?
A2: Informed consent refers to a patient’s voluntary agreement to undergo a procedure after
receiving an explanation of its purpose, risks, and benefits.
Q3: What is the purpose of the tourniquet in venipuncture?
A3: A tourniquet restricts blood flow, making veins easier to locate and puncture during
blood collection.
Q4: Define venipuncture.
A4: Venipuncture is the process of puncturing a vein to draw blood or administer
intravenous therapy.
Q5: Name two settings where a phlebotomist may work.
A5: Hospitals and outpatient clinics.
2. Infection Control
Q6: What is the most effective way to prevent the spread of infection?
A6: Hand hygiene (hand washing with soap and water or alcohol-based sanitizer) is the most
effective method.
Q7: What does PPE stand for?
A7: PPE stands for Personal Protective Equipment.
,Q8: Name two examples of PPE used in phlebotomy.
A8: Gloves and face masks.
Q9: What is the term for infection acquired in a healthcare setting?
A9: Nosocomial infection.
Q10: What should you do if you experience a needle stick injury?
A10: Immediately wash the area with soap and water, report the incident, and follow facility
protocols for exposure.
3. Safety and First Aid
Q11: What color container is used for sharps disposal?
A11: Red.
Q12: Name one emergency situation where you would use a tourniquet other than blood
draw.
A12: To control severe bleeding.
Q13: What does OSHA regulate?
A13: OSHA regulates workplace safety standards, including bloodborne pathogen exposure.
Q14: What is the first step in treating a fainting patient?
A14: Remove the tourniquet and needle, then assist the patient to a safe position.
Q15: Why should phlebotomists never recap used needles?
A15: To prevent accidental needle stick injuries.
4. Anatomy and Physiology Related to Phlebotomy
Q16: What is the function of veins in the circulatory system?
A16: Veins are blood vessels that carry deoxygenated blood back to the heart from the
body’s tissues. In phlebotomy, veins are used for blood collection because they are closer to
the surface and have lower pressure than arteries, making them safer and easier to puncture.
Q17: Name three commonly used veins for venipuncture.
A17: The three commonly used veins for venipuncture are the median cubital vein, cephalic
vein, and basilic vein. The median cubital vein is the most preferred because it is centrally
located, large, and less prone to rolling, reducing the risk of complications during blood
collection procedures.
Q18: What role do red blood cells play in the body?
A18: Red blood cells, or erythrocytes, are responsible for transporting oxygen from the lungs
to the body’s tissues and carrying carbon dioxide back to the lungs for exhalation. They
, contain hemoglobin, an iron-rich protein that binds to oxygen and gives blood its
characteristic red color.
Q19: What is plasma?
A19: Plasma is the liquid component of blood that makes up about 55% of its volume. It is a
pale yellow fluid composed mainly of water, electrolytes, proteins, hormones, and waste
products. Plasma plays a crucial role in transporting nutrients, hormones, and waste products
throughout the body.
Q20: Define homeostasis and explain its importance.
A20: Homeostasis is the body’s ability to maintain a stable internal environment despite
changes in external conditions. It is critical in regulating body temperature, pH balance, and
blood glucose levels. In phlebotomy, understanding homeostasis helps in interpreting blood
tests and recognizing abnormalities in patients.
5. Equipment and Supplies
Q21: What is the purpose of a needle gauge in phlebotomy?
A21: The needle gauge refers to the diameter of the needle's lumen. A higher gauge number
indicates a thinner needle. Phlebotomists choose different gauges based on vein size, patient
condition, and blood collection requirements to ensure a safe, efficient, and comfortable
blood draw for the patient.
Q22: Describe the use of an evacuated tube system (ETS).
A22: The evacuated tube system consists of a double-pointed needle, a plastic holder, and
collection tubes with vacuum seals. It allows for multiple blood samples to be collected
quickly and efficiently without having to repeatedly puncture the vein, improving patient
comfort and sample quality.
Q23: What is the purpose of an anticoagulant in a blood collection tube?
A23: Anticoagulants are chemicals added to certain blood collection tubes to prevent blood
from clotting. This allows for accurate testing of blood components such as plasma. Common
anticoagulants include EDTA, heparin, and sodium citrate, each selected based on the type of
laboratory analysis required.
Q24: Name two types of tourniquets and their materials.
A24: Two common types of tourniquets used in phlebotomy are latex tourniquets and
non-latex tourniquets. Latex tourniquets are made from natural rubber latex, while non-latex
versions are made from synthetic materials like neoprene or rubber alternatives, providing
options for patients with latex allergies.
Q25: What is the purpose of using gauze after venipuncture?
A25: After venipuncture, gauze is applied to the puncture site to stop bleeding and promote