100% CORRECT ANSWERS
Which of the four elements in proteins differentiates this class of substances from
carbohydrates and lipids? - correct answers ✔✔nitrogen
Which of the proteins is soluble in water?
A. albumin
B. alpha-globulins
C. beta-globulins
D. gamma-globulins - correct answers ✔✔albumin
How are amino acids joined together to form a chain-like structure? - correct answers
✔✔peptide bonding
Proteins may become denatured when subjected to mechanical agitation, heat, or chemical
treatment. What does denaturation of proteins refer to? - correct answers ✔✔alteration in
tertiary structure
Which aminoacidopathy is NOT caused by an enzyme deficiency?
A. maple syrup substitution
B. phenylketonuria
C. alkaptonuria
D. cystinuria - correct answers ✔✔Cystinuria
,How many immunoglobulin classes are currently recognized? - correct answers ✔✔five
Which reagent is employed in the serum protein determination? - correct answers ✔✔Biuret
Why should hemolyzed serum not be used for total protein analysis? - correct answers
✔✔Hemoglobin is absorbed at the same wavelength as protein in the reagent
Which of the following is NOT true of the biuret reaction?
A. follows Beer's Law for a reasonable range of protein
B. depends on the presence of at least two peptide linkages
C. is relatively free from interference by lipids and hemoglobin
D. is insensitive to the low protein levels of urine - correct answers ✔✔Is relatively free from
interference by lipids and hemoglobin
Why is bromcresol purple the preferred indicator for albumin dye binding techniques? - correct
answers ✔✔there is less interference from pigment(s)
What is the most anodic protein in electrophoresis at pH 8.6? - correct answers ✔✔albumin
The total protein is 7.2 g/dL. The serum protein results are as follows:
Albumin 53.0% 3.8g/dL
Alpha-1 3.6% 0.3g/dL
Alpha-2 11.0% 0.8g/dL
Beta 17.6% 1.2g/dL
Gamma 14.8% 1.1g/dL
,What is the A/G ratio? - correct answers ✔✔1.1
A serum protein electrophoretic pattern displaying a beta gamma bridge or broad gamma is
seen in which disease state? - correct answers ✔✔active cirrhosis
If an electrophoretic pattern shows 30% albumin, 4%-10% alpha-globulin, 4%-10% beta-
globulin, and 45% gamma-globulin, then what condition may exist in the patient? - correct
answers ✔✔monoclonal gammopathy
Glycoproteins and mucoproteins are usually bound to which substance? - correct answers
✔✔carbohydrates
All of following are glycoproteins EXCEPT which one?
A. ceruloplasmin
B. follicle-stimulating hormone
C. fibrinogen
D. cryoglobulin - correct answers ✔✔are: ceruloplasmin, follicle-stimulating hormone,
fibrinogen
EXCEPT: cryoglobulin
Which one of the following applies to cryoglobulins?
A. they are temperature-sensitive proteins
B. the specimen should clot at room temperature
C. the specimen is incubated at 22°C for 24 hours
, D. only quantitative test are available - correct answers ✔✔They are temperature-sensitive
proteins
Which of the following statements is true of albumin?
A. It makes up the smallest portion of total protein
B. Its size prevents its passage through a damaged glomerulus
C. It is produced in the liver and it is water soluble
D. Clinical problems are usually related to high serum values - correct answers ✔✔It is produced
in the liver and is water-soluble
Decreased alpha-fetoprotein values are associated with which condition?
A. Down syndrome
B. neural tube defects
C. spina bifida
D. cancer - correct answers ✔✔Down syndrome
Which one of the following proteins transports iron?
A. ceruloplasmin
B. hemopexin
C. transferrin
D. haptoglobin - correct answers ✔✔transferrin
In acute renal failure, which nonprotein nitrogen rises the fastest?