100% Correct Answers
Which of the four elements in proteins differentiated this class of substances from
carbohydrates and lipids? - correct answers ✔✔Nitrogen
Which of the proteins is soluble in water? - correct answers ✔✔Albumin
How are amino acids joined together to form a chainlike structure? - correct answers
✔✔Peptide bonding
Proteins may become denatured when subjected to mechanical agitation, heat, or chemical
treatment. What does denaturation of proteins refer to? - correct answers ✔✔Alteration of
tertiary structure
Which aminoacidopathy is NOT caused by an enzyme deficiency? - correct answers
✔✔Cystinuria
These aminoacidopathies are caused by an enzyme deficiency: - correct answers ✔✔Maple
syrup disease, phenylketonuria, and alkaptonuria
How many immunoglobulin classes are currently recognized? - correct answers ✔✔Five
Which reagent is employed in the serum protein determination? - correct answers ✔✔Biuret
Why should hemolyzed serum not be used for total protein analysis? - correct answers
✔✔Hemoglobin is absorbed at the same wavelength as protein in the reagent
,Which of the following is NOT true of the biuret reaction? - correct answers ✔✔It is relatively
free from interference from lipids and hemoglobin
Which of the following is true of the biuret reaction? - correct answers ✔✔Follows Beer's law
for a reasonable range of protein, depends on the presence of at least two peptide linkages, and
is insensitive to the low protein levels of urine.
Why is bromcresol purple the preferred indicator for albumin dye binding techniques? - correct
answers ✔✔There is less interference from pigment(s)
What is the most anodic protein on electrophoresis at pH 8.6? - correct answers ✔✔Albumin
The total protein is 7.1 g/dL. The serum protein results are:
Albumin 53.0% 3.8 g/dL
Alpha1 3.6% 0.3 g/dL
Alpha2 11.0% 0.8 g/dL
Beta 17.6% 1.2 g/dL
Gamma 14.8% 1.1 g/dL
What is the A/G ratio? - correct answers ✔✔3.8/(.3+.8+1.2+1.1) = 1.1
A serum protein electrophoretic pattern displaying a beta gamma bridge or broad gamma is
seen in which disease state? - correct answers ✔✔Active cirrhosis
If an electrophoretic pattern shows 30% albumin, 4%-10% alpha-globulin, 4%-10% beta-
globulin, and 45% gamma globulin, then what condition may exist in the patient? - correct
answers ✔✔Monoclonal gammopathy
, Glycoproteins and mucoproteins are usually bound to which substance? - correct answers
✔✔Carbohydrates
All of the following are glycoproteins: - correct answers ✔✔Ceruloplasmin, follicle-stimulating
hormone (FSH), and fibrinogen
All of the following are glycoproteins EXCEPT: - correct answers ✔✔Cryoglobulin
Which one of the following applies to cryoglobulins? - correct answers ✔✔They are
temperature-sensitive proteins
Which of the following statements is true of albumin? - correct answers ✔✔It is produced in the
liver and is water-soluble
Decreased alpha-fetoprotein values are associated with which condition? - correct answers
✔✔Down syndrome
Which one of the following proteins transports iron? - correct answers ✔✔Transferrin
In acute renal failure, which nonprotein nitrogen rises the fastest? - correct answers ✔✔Blood
urea nitrogen (BUN)
By the urease method, urea is enzymatically converted to which end product? - correct answers
✔✔Ammonia
Why can untreated urine be used for the determination of urea by the diacetyl monoxomine
method? - correct answers ✔✔The method is not measuring ammonia