BCHM 307 Final Exam/ Actual Exam Questions with Correct Veri
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fied Answers- Latest Update 2024 – Rated A+
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Which#of#the#following#describes#the#isomerization#of#citrate#to#isocitrate?#-#ANSWER#-
#converts#a#tertiary#alcohol,#which#cannot#easily#be#oxidized,#to#a#secondary#alcohol#that#can#be#oxidized
What#is#the#purpose#of#DNA#helicase?#-#ANSWER#-
#separation#of#double#stranded#DNA#into#single#stranded#DNA#in#front#of#DNA#polymerase
Which#of#the#following#mutations#would#cause#a#bulge#in#the#DNA#backbone?#-#ANSWER#-
#transversion#mutation
What#is#the#nucleophile#in#the#DNA#polymerase#mechanism?#-#ANSWER#-
#the#3'#hydroxyl#on#the#deoxyribose
During#the#first#half#of#the#chymotrypsin#mechanism#where#the#acyl-
enzyme#intermediate#is#formed,#what#role#does#His#play?#-#ANSWER#-#general#base#then#general#acid
How#many#cytochrome#c#molecules#are#oxidized#by#Complex#IV#for#each#molecule#of#oxygen#that#is#reduc
ed#-#ANSWER#-#4
Which#of#the#following#occurs#when#the#catalytic#subunit#of#ATP#synthase#is#in#the#loose#state?#-#ANSWER
-#ADP#and#Pi#bind
Why#is#the#occasional#production#of#the#superoxide#radical#anion#by#complex#IV#of#the#electron#transport#
chain#problematic?#-#ANSWER#-#It#is#highly#reactive#and#can#damage#nearby#biomolecules.
Which#of#the#following#can#happen#when#a#photosynthetic#pigment#absorbs#light?#-#ANSWER#-
#energy#is#lost#as#heat
pg.#1
,b.#energy#is#given#of#f#as#fluorescence
c.#exciton#transfer#to#a#neighboring#pigment
If#the#reduction#potential#for#NAD+#is#-
0.315#V#and#the#reduction#potential#for#oxygen#is#0.815#V,#what#is#the#potential#for#the#oxidation#of#NAD
H#by#oxygen?#-#ANSWER#-#1.13#V
Which#of#the#following#transport#systems#is#used#in#the#inner#mitochondrial#membrane?#IMS#=
intermembrane#space#-#ANSWER#-#antiport#of#ADP#from#IMS#to#matrix#and#of#ATP#from#matrix#to#IMS
The#highest#point#in#a#reaction#coordinate#diagram#represents# .#-#ANSWER#-#the#transition#state
Of#the#following#ions,#which#would#be#most#likely#to#participate#in#metal#ion#catalysis?#-#ANSWER#-#Zn2+
An#organic#molecule#that#is#tightly#bound#to#an#enzyme#and#participates#in#an#enzyme#catalyzed#reaction
#is#specifically#referred#to#as#a# .#-#ANSWER#-#prosthetic#group
Which#of#the#following#amino#acids#would#be#most#likely#found#in#the#active#site#of#an#enzyme#that#uses
#acid-base#catalysis?#-#ANSWER#-#His
The#ability#for#an#enzyme#to#pick#out#one#particular#substrate#from#the#myriad#of#molecules#floating
#around#its#environment#is#an#example#of# .#-#ANSWER#-#specificity
How#does#a#catalyst#affect#the#overall#deltaG#of#an#endergonic#reaction?#-#ANSWER#-#it#has#no#effect
Which#of#the#following#is#the#most#effective#way#to#increase#the#rate#of#a#biochemical#reaction?#-
#ANSWER#-#add#a#catalyst
How#does#a#catalyst#increase#the#rate#of#a#reaction?#-#ANSWER#-
#it#allows#reacting#molecules#to#more#easily#form#the#transition#state
Of#all#the#species#that#enzymes#bind,#they#are#thought#to#bind#most#tightly#to# .#-#ANSWER#-
#transition#states
pg.#2
, Chymotrypsin#catalyzes#the#hydrolysis#of#a#peptide#bond#and#is#therefore#categorized#as#a# .#-
#ANSWER#-#hydrolase
Chymotrypsin#has#a#large#specificity#pocket#to#bind#the#aromatic#amino#acids#while#elastase#has#a#very#sm
all#specificity#pocket#meant#to#bind#Ala#or#Gly.#Which#of#the#following#amino#acid#substitutions
changes#the#specificity#pocket#of#chymotrypsin#to#that#of#elastase?#(Try#to#figure#this#out#based#on#amino#
acid#structures#rather#than#just#looking#it#up!)#-#ANSWER#-#Gly#->#Val
If#the#Asp#in#the#chymotrypsin#active#site#was#mutated#to#another#amino#acid,#which#of#the#following#wo
uld#be#considered#an#invisible#mutation#in#that#it#is#least#likely#to#impact#the#function#of#the#enzyme?#-
#ANSWER#-#Asp#→#Glu
Zymogens#are#not#enzymatically#active#because# .#-#ANSWER#-
#their#active#sites#are#distorted#and#incapable#of#enzymatic#activity
The#molecule#chymotrypsinogen#is#known#as#a(n)# .#-#ANSWER#-#zymogen
What#three#amino#acids#are#found#in#the#catalytic#triad#of#chymotrypsin?#-#ANSWER#-#Asp,#His,#Ser
Conversion#of#factor#X#to#factor#Xa#represents#a(n)#
form#of#activation;#binding#of#antithrombin#to#thrombin#represents#a(n
)# form#of#inhibition.#-#ANSWER#-#irreversible;#reversible
An#enzyme#that#forms#a#covalent#bond#with#its#substrate#during#the#course#of#a#reaction#is#considered#to
#undergo# .#-#ANSWER#-#covalent#catalysis
Which#protein#in#the#blood#is#responsible#for#converting#fibrinogen#to#fibrin?#-#ANSWER#-#thrombin
Different#enzymes#that#catalyze#the#same#reaction#are#known#as# .#-#ANSWER#-#isoenzymes
In#an#enzyme#mechanism#that#generates#a#negative#charge#in#the#transition#state,#which#of#the#following#
would#be#most#effective#to#have#in#the#active#site#of#the#enzyme?#-#ANSWER#-#transition#metal#cation
pg.#3
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fied Answers- Latest Update 2024 – Rated A+
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Which#of#the#following#describes#the#isomerization#of#citrate#to#isocitrate?#-#ANSWER#-
#converts#a#tertiary#alcohol,#which#cannot#easily#be#oxidized,#to#a#secondary#alcohol#that#can#be#oxidized
What#is#the#purpose#of#DNA#helicase?#-#ANSWER#-
#separation#of#double#stranded#DNA#into#single#stranded#DNA#in#front#of#DNA#polymerase
Which#of#the#following#mutations#would#cause#a#bulge#in#the#DNA#backbone?#-#ANSWER#-
#transversion#mutation
What#is#the#nucleophile#in#the#DNA#polymerase#mechanism?#-#ANSWER#-
#the#3'#hydroxyl#on#the#deoxyribose
During#the#first#half#of#the#chymotrypsin#mechanism#where#the#acyl-
enzyme#intermediate#is#formed,#what#role#does#His#play?#-#ANSWER#-#general#base#then#general#acid
How#many#cytochrome#c#molecules#are#oxidized#by#Complex#IV#for#each#molecule#of#oxygen#that#is#reduc
ed#-#ANSWER#-#4
Which#of#the#following#occurs#when#the#catalytic#subunit#of#ATP#synthase#is#in#the#loose#state?#-#ANSWER
-#ADP#and#Pi#bind
Why#is#the#occasional#production#of#the#superoxide#radical#anion#by#complex#IV#of#the#electron#transport#
chain#problematic?#-#ANSWER#-#It#is#highly#reactive#and#can#damage#nearby#biomolecules.
Which#of#the#following#can#happen#when#a#photosynthetic#pigment#absorbs#light?#-#ANSWER#-
#energy#is#lost#as#heat
pg.#1
,b.#energy#is#given#of#f#as#fluorescence
c.#exciton#transfer#to#a#neighboring#pigment
If#the#reduction#potential#for#NAD+#is#-
0.315#V#and#the#reduction#potential#for#oxygen#is#0.815#V,#what#is#the#potential#for#the#oxidation#of#NAD
H#by#oxygen?#-#ANSWER#-#1.13#V
Which#of#the#following#transport#systems#is#used#in#the#inner#mitochondrial#membrane?#IMS#=
intermembrane#space#-#ANSWER#-#antiport#of#ADP#from#IMS#to#matrix#and#of#ATP#from#matrix#to#IMS
The#highest#point#in#a#reaction#coordinate#diagram#represents# .#-#ANSWER#-#the#transition#state
Of#the#following#ions,#which#would#be#most#likely#to#participate#in#metal#ion#catalysis?#-#ANSWER#-#Zn2+
An#organic#molecule#that#is#tightly#bound#to#an#enzyme#and#participates#in#an#enzyme#catalyzed#reaction
#is#specifically#referred#to#as#a# .#-#ANSWER#-#prosthetic#group
Which#of#the#following#amino#acids#would#be#most#likely#found#in#the#active#site#of#an#enzyme#that#uses
#acid-base#catalysis?#-#ANSWER#-#His
The#ability#for#an#enzyme#to#pick#out#one#particular#substrate#from#the#myriad#of#molecules#floating
#around#its#environment#is#an#example#of# .#-#ANSWER#-#specificity
How#does#a#catalyst#affect#the#overall#deltaG#of#an#endergonic#reaction?#-#ANSWER#-#it#has#no#effect
Which#of#the#following#is#the#most#effective#way#to#increase#the#rate#of#a#biochemical#reaction?#-
#ANSWER#-#add#a#catalyst
How#does#a#catalyst#increase#the#rate#of#a#reaction?#-#ANSWER#-
#it#allows#reacting#molecules#to#more#easily#form#the#transition#state
Of#all#the#species#that#enzymes#bind,#they#are#thought#to#bind#most#tightly#to# .#-#ANSWER#-
#transition#states
pg.#2
, Chymotrypsin#catalyzes#the#hydrolysis#of#a#peptide#bond#and#is#therefore#categorized#as#a# .#-
#ANSWER#-#hydrolase
Chymotrypsin#has#a#large#specificity#pocket#to#bind#the#aromatic#amino#acids#while#elastase#has#a#very#sm
all#specificity#pocket#meant#to#bind#Ala#or#Gly.#Which#of#the#following#amino#acid#substitutions
changes#the#specificity#pocket#of#chymotrypsin#to#that#of#elastase?#(Try#to#figure#this#out#based#on#amino#
acid#structures#rather#than#just#looking#it#up!)#-#ANSWER#-#Gly#->#Val
If#the#Asp#in#the#chymotrypsin#active#site#was#mutated#to#another#amino#acid,#which#of#the#following#wo
uld#be#considered#an#invisible#mutation#in#that#it#is#least#likely#to#impact#the#function#of#the#enzyme?#-
#ANSWER#-#Asp#→#Glu
Zymogens#are#not#enzymatically#active#because# .#-#ANSWER#-
#their#active#sites#are#distorted#and#incapable#of#enzymatic#activity
The#molecule#chymotrypsinogen#is#known#as#a(n)# .#-#ANSWER#-#zymogen
What#three#amino#acids#are#found#in#the#catalytic#triad#of#chymotrypsin?#-#ANSWER#-#Asp,#His,#Ser
Conversion#of#factor#X#to#factor#Xa#represents#a(n)#
form#of#activation;#binding#of#antithrombin#to#thrombin#represents#a(n
)# form#of#inhibition.#-#ANSWER#-#irreversible;#reversible
An#enzyme#that#forms#a#covalent#bond#with#its#substrate#during#the#course#of#a#reaction#is#considered#to
#undergo# .#-#ANSWER#-#covalent#catalysis
Which#protein#in#the#blood#is#responsible#for#converting#fibrinogen#to#fibrin?#-#ANSWER#-#thrombin
Different#enzymes#that#catalyze#the#same#reaction#are#known#as# .#-#ANSWER#-#isoenzymes
In#an#enzyme#mechanism#that#generates#a#negative#charge#in#the#transition#state,#which#of#the#following#
would#be#most#effective#to#have#in#the#active#site#of#the#enzyme?#-#ANSWER#-#transition#metal#cation
pg.#3
