MLS PROGRAM COMPREHENSIVE EXAM QUESTIONS AND
ANSWERS
What antibody is most associated with delayed hemolytic transfusion reactions? - CORRECT
ANSWER✅✅✅Anti-Jka
What antibody is associated with Mycoplasma pneumonia? - CORRECT ANSWER✅✅✅Anti-I
What class of antibodies cause extravascular hemolysis from sequestration by spleen? - CORRECT
ANSWER✅✅✅IgG
Enzyme panel will not react with which antibodes? - CORRECT ANSWER✅✅✅Duffy (Fya or Fyb)
What can cause a mother to have a low titer anti-D? - CORRECT ANSWER✅✅✅RhIg at 28 weeks
(T/F) Blood from mom to child must be irradiated. - CORRECT ANSWER✅✅✅True
What kind of errors can room temp cross match detect? - CORRECT ANSWER✅✅✅ABO
What is the main reason premature infants need transfusions? - CORRECT ANSWER✅✅✅Blood loss
from lab tests (aka iatrogenic blood loss)
What type of positive control should be used when phenotyping? - CORRECT
ANSWER✅✅✅Heterozyous
What could make the auto control positive? - CORRECT ANSWER✅✅✅Rouleaux or cold agglutinin
How would you correct rouleaux? - CORRECT ANSWER✅✅✅Saline replacement
How would you fix cold agglutinin results? - CORRECT ANSWER✅✅✅Prewarm
,Cryoprecipitate is used today mostly to replace what? - CORRECT ANSWER✅✅✅Fibrinogen
What type of plasma can an AB = patient receive? - CORRECT ANSWER✅✅✅only AB
What are the requirements for intrauterine transfusions? - CORRECT ANSWER✅✅✅Fresh, O neg,
CMV neg, irradiated, collected with CPDA
When is FFP used? - CORRECT ANSWER✅✅✅When PT and PTT are prolonged
What temperature do Lewis antibodies react? - CORRECT ANSWER✅✅✅RT and sometimes 37
What affect can Lewis antibodies have on samples? - CORRECT ANSWER✅✅✅Can cause in vitro
hemolysis
How long would a sexual partner of an IV drug user be deferred from donating? - CORRECT
ANSWER✅✅✅One year
What can make the Rh control positive when doing a weak D test? - CORRECT
ANSWER✅✅✅Positive DAT
What medication does PT monitor? - CORRECT ANSWER✅✅✅Coumadin (Warfarin)
Protein C deficiency causes what? - CORRECT ANSWER✅✅✅Thrombosis
What factor deficiency causes thrombosis instead of bleeding? - CORRECT ANSWER✅✅✅XII
What parameter does a cold agglutinin NOT affect? - CORRECT ANSWER✅✅✅Hemoglobin
, What will the cells of an aged sample look like? - CORRECT ANSWER✅✅✅crenated red cells and
vacuoles in white cells
What two RBC morphologies will lower a sed rate? - CORRECT ANSWER✅✅✅Sickle cells and
spherocytes
What changes should be made to a PT or PTT sample collected on a patient with polcythemia? -
CORRECT ANSWER✅✅✅Anticoagulant should be adjusted to correct for less plasma
Megaloblastic anemia is associated with what? - CORRECT ANSWER✅✅✅Hypersegmented
neutrophils, pancytopenia, and macrocytes
What are Dohle bodies? - CORRECT ANSWER✅✅✅Blue oblong inclusions located near the periphery
of seg
What are Dohle bodies composed of and when are they seen? - CORRECT ANSWER✅✅✅RNA or
rough endoplasmic reticulum; Bacterial infection, inflammation, or burns
What are Howell-Jolly bodies composed of and when are they seen? - CORRECT ANSWER✅✅✅DNA;
megaloblastic anemia, splenomegaly or splenectomy, thalassemia, sickle cell, or hemolytic anemia
What is basophilic stippling and what does it indicate/when is it seen? - CORRECT
ANSWER✅✅✅Denatured RNA; altered or impaired hemoglobin synthesis/lead poisoning,
thalassemia
What are Pappenheimer bodies called when Prussian blue stain is used? - CORRECT
ANSWER✅✅✅Siderocytes
What are Pappenheimer bodies composed of and when are they seen? - CORRECT
ANSWER✅✅✅Iron; sideroblastic anemia (iron overload), thalassemia, splenectomy
ANSWERS
What antibody is most associated with delayed hemolytic transfusion reactions? - CORRECT
ANSWER✅✅✅Anti-Jka
What antibody is associated with Mycoplasma pneumonia? - CORRECT ANSWER✅✅✅Anti-I
What class of antibodies cause extravascular hemolysis from sequestration by spleen? - CORRECT
ANSWER✅✅✅IgG
Enzyme panel will not react with which antibodes? - CORRECT ANSWER✅✅✅Duffy (Fya or Fyb)
What can cause a mother to have a low titer anti-D? - CORRECT ANSWER✅✅✅RhIg at 28 weeks
(T/F) Blood from mom to child must be irradiated. - CORRECT ANSWER✅✅✅True
What kind of errors can room temp cross match detect? - CORRECT ANSWER✅✅✅ABO
What is the main reason premature infants need transfusions? - CORRECT ANSWER✅✅✅Blood loss
from lab tests (aka iatrogenic blood loss)
What type of positive control should be used when phenotyping? - CORRECT
ANSWER✅✅✅Heterozyous
What could make the auto control positive? - CORRECT ANSWER✅✅✅Rouleaux or cold agglutinin
How would you correct rouleaux? - CORRECT ANSWER✅✅✅Saline replacement
How would you fix cold agglutinin results? - CORRECT ANSWER✅✅✅Prewarm
,Cryoprecipitate is used today mostly to replace what? - CORRECT ANSWER✅✅✅Fibrinogen
What type of plasma can an AB = patient receive? - CORRECT ANSWER✅✅✅only AB
What are the requirements for intrauterine transfusions? - CORRECT ANSWER✅✅✅Fresh, O neg,
CMV neg, irradiated, collected with CPDA
When is FFP used? - CORRECT ANSWER✅✅✅When PT and PTT are prolonged
What temperature do Lewis antibodies react? - CORRECT ANSWER✅✅✅RT and sometimes 37
What affect can Lewis antibodies have on samples? - CORRECT ANSWER✅✅✅Can cause in vitro
hemolysis
How long would a sexual partner of an IV drug user be deferred from donating? - CORRECT
ANSWER✅✅✅One year
What can make the Rh control positive when doing a weak D test? - CORRECT
ANSWER✅✅✅Positive DAT
What medication does PT monitor? - CORRECT ANSWER✅✅✅Coumadin (Warfarin)
Protein C deficiency causes what? - CORRECT ANSWER✅✅✅Thrombosis
What factor deficiency causes thrombosis instead of bleeding? - CORRECT ANSWER✅✅✅XII
What parameter does a cold agglutinin NOT affect? - CORRECT ANSWER✅✅✅Hemoglobin
, What will the cells of an aged sample look like? - CORRECT ANSWER✅✅✅crenated red cells and
vacuoles in white cells
What two RBC morphologies will lower a sed rate? - CORRECT ANSWER✅✅✅Sickle cells and
spherocytes
What changes should be made to a PT or PTT sample collected on a patient with polcythemia? -
CORRECT ANSWER✅✅✅Anticoagulant should be adjusted to correct for less plasma
Megaloblastic anemia is associated with what? - CORRECT ANSWER✅✅✅Hypersegmented
neutrophils, pancytopenia, and macrocytes
What are Dohle bodies? - CORRECT ANSWER✅✅✅Blue oblong inclusions located near the periphery
of seg
What are Dohle bodies composed of and when are they seen? - CORRECT ANSWER✅✅✅RNA or
rough endoplasmic reticulum; Bacterial infection, inflammation, or burns
What are Howell-Jolly bodies composed of and when are they seen? - CORRECT ANSWER✅✅✅DNA;
megaloblastic anemia, splenomegaly or splenectomy, thalassemia, sickle cell, or hemolytic anemia
What is basophilic stippling and what does it indicate/when is it seen? - CORRECT
ANSWER✅✅✅Denatured RNA; altered or impaired hemoglobin synthesis/lead poisoning,
thalassemia
What are Pappenheimer bodies called when Prussian blue stain is used? - CORRECT
ANSWER✅✅✅Siderocytes
What are Pappenheimer bodies composed of and when are they seen? - CORRECT
ANSWER✅✅✅Iron; sideroblastic anemia (iron overload), thalassemia, splenectomy
