MCB 2050 Midterm Review Questions and
Answers 100% Solved
The technique used to identify a specific protein band following electrophoresis in a
polyacrylamide gel: Western blotting
The technique used to study protein localization in fixed cells is called:
Immunofluorescence
What is the name of a fluorescent protein that is often fused to a protein of interest to follow the
localization of the protein of interest? Green fluorescent protein (GFP)
What is the advantage of using GFP-tagging as opposed to immunofluorescence imaging to
study protein localization? GFP-tagged proteins can be visualized in living cells in real
time over extended periods of time. Immunofluorescence imaging only gives you a temporal
snapshot.
Use of two different GFP-tagged proteins each having different colours allows you to determine
what about the localization of these proteins? Whether or not they are co-localized within
the cell
,The type of sequencing that depends on chain termination using nucleotides that lack a 3' OH
group Dideoxy (Sanger) sequencing
TOPIC 2
The study of all proteins in a cell is called: Proteomics
The study of global transcription for a given genome is called: Transcriptomics
The study of the interactions of different components in a cell is called: Interactomics
A map of a chromosome based on fluorescent in situ hybridization with known DNA probes
Cytogenetic map
The process of using a fluorescent DNA probe to map on a chromosome the location of the gene
corresponding to the probe Fluorescent in situ hybridization (FISH)
, The name of the process where you align restriction endonuclease sites to specific and accurate
locations on a piece of DNA Physical Map
A map made of overlapping DNA clones is called Contig Map
The name of a set of SNPs on the same chromosome that tend to be inherited together
Haplotype
DNA sequences that are mapped both genetically and physically on a chromosome are called
Anchor markers
Sequencing approach where sequencing is done in an ordered way in which a DNA cloned
adjacent to an already cloned sequence is used to advance the sequence beyond that region and
etc. Chromosome walking
What is the name of the strategy used by Celera to sequence the human genome? Shot-
gun sequencing
Answers 100% Solved
The technique used to identify a specific protein band following electrophoresis in a
polyacrylamide gel: Western blotting
The technique used to study protein localization in fixed cells is called:
Immunofluorescence
What is the name of a fluorescent protein that is often fused to a protein of interest to follow the
localization of the protein of interest? Green fluorescent protein (GFP)
What is the advantage of using GFP-tagging as opposed to immunofluorescence imaging to
study protein localization? GFP-tagged proteins can be visualized in living cells in real
time over extended periods of time. Immunofluorescence imaging only gives you a temporal
snapshot.
Use of two different GFP-tagged proteins each having different colours allows you to determine
what about the localization of these proteins? Whether or not they are co-localized within
the cell
,The type of sequencing that depends on chain termination using nucleotides that lack a 3' OH
group Dideoxy (Sanger) sequencing
TOPIC 2
The study of all proteins in a cell is called: Proteomics
The study of global transcription for a given genome is called: Transcriptomics
The study of the interactions of different components in a cell is called: Interactomics
A map of a chromosome based on fluorescent in situ hybridization with known DNA probes
Cytogenetic map
The process of using a fluorescent DNA probe to map on a chromosome the location of the gene
corresponding to the probe Fluorescent in situ hybridization (FISH)
, The name of the process where you align restriction endonuclease sites to specific and accurate
locations on a piece of DNA Physical Map
A map made of overlapping DNA clones is called Contig Map
The name of a set of SNPs on the same chromosome that tend to be inherited together
Haplotype
DNA sequences that are mapped both genetically and physically on a chromosome are called
Anchor markers
Sequencing approach where sequencing is done in an ordered way in which a DNA cloned
adjacent to an already cloned sequence is used to advance the sequence beyond that region and
etc. Chromosome walking
What is the name of the strategy used by Celera to sequence the human genome? Shot-
gun sequencing
