Diagnostic Microbiology, 15th Edition
Covering Chapters 1-76 | Includes
Questions With Verified Answers &
Rationales
_____________________________________________________________________________________
B
Specific proteins produced by cells of the immune system in response to the presence of foreign
molecules that circulate in the serum and are present in secretions such as saliva are called
A) Antigens
B) Immunoglobulins
C) Cytokines
D) Phagogens
B
If this is present on a surface to be disinfected, it can shield microorganisms from the disinfectant or
inactivate the disinfectant. What is this substance?
A) Bleach
B) Organic material
C) Hydrochloric acid
D) Water
c
Universal/Standard Precautions require that
A) Only some body fluids be considered infectious and capable of transmitting disease
B) Body fluids with visible blood be treated as noninfectious
C) Blood and body fluids from all patients be considered infectious and capable of transmitting disease
D) Urine and feces be considered noninfectious
,D
Because Biosafety Level 3 (BSL-3) organisms have the potential for aerosol transmission, and diseases
with these agents may have serious lethal consequences, all of the following guidelines apply to BSL-3
labs EXCEPT:
A) BSL-3 laboratories should be separated from others parts of the building by an anteroom
B) BSL-3 laboratories should have restricted access with documentation of personnel entering and
exiting the laboratory
C) BSL-3 laboratories should have solid ceiling and floor seams, and any seams must be sealed
D) BSL-3 laboratories should have positive air pressure
C
Contaminated needles and syringes without safety self sheathing devices should be:
A) Sheared by a needle cutter or bent
B) re-capped using a two handed technique
C) Discarded directly into an appropriate sharps container
D) Removed from the syringe/needle holder
D
The specimen of choice for detecting gastrointestinal pathogens is
A) A urine specimen
B) Vomit
C) A swab of the anal area with no feces on it
D) A stool specimen
B
To perform a quality laboratory analysis, the laboratory needs specific information regarding the
patient and the specimen. What can be a critical weak link in the specimen management process?
A) Poor specimen collection techniques
,B) Incomplete information on the requisition
C) Poor-quality hospital information system
D) Poor-quality laboratory information system
D
Selective media
A) Allow the growth of fastidious microbes through the addition of certain growth enhancers
B) Support the grouping of microbes based on different characteristics demonstrated in the media
C) Support the growth of most nonfastidious microbes
D) Support the growth of one type or group of microbes, but not another
D
How is culture media selected for specimen plating?
A) Determine the supplemental agar needed, as well as the broth media
B) Always include nonselective media and differential media to help save a day of reading the culture on
the bench
C) Use only broth for the initial inoculation and subculture to plates after 24 hours of incubation
D) The selection of media to inoculate is based on the type of specimen submitted for culture and the
organisms likely to be involved in the infectious process
C
What technique is used to isolate bacterial colonies when inoculating organisms onto an agar plate?
A) Homogenization
B) Centrifugation
C) Isolation streaking
D) Culture streaking
C
To help the microbiologist report microbiology results to the physician in a timely fashion to ensure
rapid, appropriate treatment, the technician may use this report
, A) Antibiogram
B) Infection control summary
C) Preliminary
D) Final
C
What work practice have microbiologists incorporated to keep laboratory testing cost-effective while
providing optimum patient care?
A) Using definitive identification for the standard of quality patient care
B) Always identifying organisms to the maximum extent
C) Limited identification procedures
D) Limited antimicrobial susceptibility procedures
E
A bacterial smear may indicate a polymicrobial infection when
A) Mucus, red blood cells, neutrophjils, and necrosis are on the smear
B) lymphocytes, monocytes, and macrophages are on the smear
C) More than one morphological type of bacteria is present on the smear
D) Both gram-negative rods and gram-positive cocci are present on the smear
E) C and D
F) A and B
D
What elements should be included in the direct examination of a microbial smear such as in the
evaluation of a sputum sample?
A) Only those elements useful in characterizing the specimen
B) Only elements pertaining to the necrosis present int he specimen
C) Only those elements pertaining to the amount of gram-positive cocci present int he specimen
D) Only those elements of contaminating material present in the specimen
Covering Chapters 1-76 | Includes
Questions With Verified Answers &
Rationales
_____________________________________________________________________________________
B
Specific proteins produced by cells of the immune system in response to the presence of foreign
molecules that circulate in the serum and are present in secretions such as saliva are called
A) Antigens
B) Immunoglobulins
C) Cytokines
D) Phagogens
B
If this is present on a surface to be disinfected, it can shield microorganisms from the disinfectant or
inactivate the disinfectant. What is this substance?
A) Bleach
B) Organic material
C) Hydrochloric acid
D) Water
c
Universal/Standard Precautions require that
A) Only some body fluids be considered infectious and capable of transmitting disease
B) Body fluids with visible blood be treated as noninfectious
C) Blood and body fluids from all patients be considered infectious and capable of transmitting disease
D) Urine and feces be considered noninfectious
,D
Because Biosafety Level 3 (BSL-3) organisms have the potential for aerosol transmission, and diseases
with these agents may have serious lethal consequences, all of the following guidelines apply to BSL-3
labs EXCEPT:
A) BSL-3 laboratories should be separated from others parts of the building by an anteroom
B) BSL-3 laboratories should have restricted access with documentation of personnel entering and
exiting the laboratory
C) BSL-3 laboratories should have solid ceiling and floor seams, and any seams must be sealed
D) BSL-3 laboratories should have positive air pressure
C
Contaminated needles and syringes without safety self sheathing devices should be:
A) Sheared by a needle cutter or bent
B) re-capped using a two handed technique
C) Discarded directly into an appropriate sharps container
D) Removed from the syringe/needle holder
D
The specimen of choice for detecting gastrointestinal pathogens is
A) A urine specimen
B) Vomit
C) A swab of the anal area with no feces on it
D) A stool specimen
B
To perform a quality laboratory analysis, the laboratory needs specific information regarding the
patient and the specimen. What can be a critical weak link in the specimen management process?
A) Poor specimen collection techniques
,B) Incomplete information on the requisition
C) Poor-quality hospital information system
D) Poor-quality laboratory information system
D
Selective media
A) Allow the growth of fastidious microbes through the addition of certain growth enhancers
B) Support the grouping of microbes based on different characteristics demonstrated in the media
C) Support the growth of most nonfastidious microbes
D) Support the growth of one type or group of microbes, but not another
D
How is culture media selected for specimen plating?
A) Determine the supplemental agar needed, as well as the broth media
B) Always include nonselective media and differential media to help save a day of reading the culture on
the bench
C) Use only broth for the initial inoculation and subculture to plates after 24 hours of incubation
D) The selection of media to inoculate is based on the type of specimen submitted for culture and the
organisms likely to be involved in the infectious process
C
What technique is used to isolate bacterial colonies when inoculating organisms onto an agar plate?
A) Homogenization
B) Centrifugation
C) Isolation streaking
D) Culture streaking
C
To help the microbiologist report microbiology results to the physician in a timely fashion to ensure
rapid, appropriate treatment, the technician may use this report
, A) Antibiogram
B) Infection control summary
C) Preliminary
D) Final
C
What work practice have microbiologists incorporated to keep laboratory testing cost-effective while
providing optimum patient care?
A) Using definitive identification for the standard of quality patient care
B) Always identifying organisms to the maximum extent
C) Limited identification procedures
D) Limited antimicrobial susceptibility procedures
E
A bacterial smear may indicate a polymicrobial infection when
A) Mucus, red blood cells, neutrophjils, and necrosis are on the smear
B) lymphocytes, monocytes, and macrophages are on the smear
C) More than one morphological type of bacteria is present on the smear
D) Both gram-negative rods and gram-positive cocci are present on the smear
E) C and D
F) A and B
D
What elements should be included in the direct examination of a microbial smear such as in the
evaluation of a sputum sample?
A) Only those elements useful in characterizing the specimen
B) Only elements pertaining to the necrosis present int he specimen
C) Only those elements pertaining to the amount of gram-positive cocci present int he specimen
D) Only those elements of contaminating material present in the specimen
