BIO 175 Exam 1 Questions with Complete
Solutions
magnification Microscopes achieve ______________________ through objective and
ocular lens, light rays, and refraction.
resolution capacity of an optical system to distinguish two adjacent objects
oil immersion 100x magnification, captures light that would otherwise be scattered
slide smear prep Depends on:
condition of specimen-living or preserved?
the goals of the examiner
type of microscopy available
negative staining staining the background instead of the cell
- charge
,positive staining the dye sticks to the specimen to give it color
+ charge
gram stain Universal basis staining method that distinguishes between two different kinds
of bacterial cell walls.
1884 Hans Gram
Red= negative
Purple= positive
acid fast stain A staining procedure for identifying bacteria that have a waxy (mycolic
acids) cell wall.
Pink= resistant to acid
Blue= non-resistant to acid
endospore stain is applicable to only a few groups of bacteria AND usually shows the
spores as green structures among a background of pink cells.
, Special stain Stains such as endospore staining and flagella staining.
internal structures of prokaryotes - cytoplasmic membrane
- cytoplasm
- endospores
- ribosomes
-inclusions: stored nutrients
-plasmid
-microcompartments: protein packets to localize enzymes/proteins into cytoplasm
boundary structures of prokaryotes outer membrane
cell wall
cytoplasmic membrane
external structures of prokaryotes flagella
pili
fimbriae
Solutions
magnification Microscopes achieve ______________________ through objective and
ocular lens, light rays, and refraction.
resolution capacity of an optical system to distinguish two adjacent objects
oil immersion 100x magnification, captures light that would otherwise be scattered
slide smear prep Depends on:
condition of specimen-living or preserved?
the goals of the examiner
type of microscopy available
negative staining staining the background instead of the cell
- charge
,positive staining the dye sticks to the specimen to give it color
+ charge
gram stain Universal basis staining method that distinguishes between two different kinds
of bacterial cell walls.
1884 Hans Gram
Red= negative
Purple= positive
acid fast stain A staining procedure for identifying bacteria that have a waxy (mycolic
acids) cell wall.
Pink= resistant to acid
Blue= non-resistant to acid
endospore stain is applicable to only a few groups of bacteria AND usually shows the
spores as green structures among a background of pink cells.
, Special stain Stains such as endospore staining and flagella staining.
internal structures of prokaryotes - cytoplasmic membrane
- cytoplasm
- endospores
- ribosomes
-inclusions: stored nutrients
-plasmid
-microcompartments: protein packets to localize enzymes/proteins into cytoplasm
boundary structures of prokaryotes outer membrane
cell wall
cytoplasmic membrane
external structures of prokaryotes flagella
pili
fimbriae
