MLT ASCP BOC EXAM SCRIPT 2025/2026 QUESTIONS WITH
ANSWERS GRADED A+
✔✔Which of the following serological tests would be used for the diagnosis of Q-fever?
1. Wail-Felix test
2. Quellung test
3. EIA or indirect immunoflourescence
4. Cold agglutinin test - ✔✔3. EIA or indirect immunoflourescence
✔✔With most light microscopes, what is the total magnification if using the objective
lens that is closest to the specimen along with the ocular lens?
1. 100X
2. 200X
3. 400X
4. 1000X - ✔✔1. 100X
✔✔Which of the following methods may be employed to definitively identify Bence-
Jones proteins?
1. Immunoelectrophoresis
2. Sulfosalicyclic acid precipitation
3. Heat precipitation at 40-60 C
4. Urine dipstick - ✔✔1. Immunoelectrophoresis
✔✔Nephelometry is based on the principle of:
1. Turbidity resulting from specific antigen-coated latex particles agglutinated by
corresponding antibody
2. Agglutination resulting from physical attachment of antibody molecules to antigens on
an erythrocyte membrane.
3. Agglutination of host antibody to antigenic determinants on a bacterial agent
4. Precipitation of immune-related proteins in agarose gel - ✔✔1. Turbidity resulting
from specific antigen-coated latex particles agglutinated by corresponding antibody
✔✔Patients with which of the following conditions would benefit most from washed red
cells:
1. Warm autoimmune hemolytic anemia
2. Cold autoimmune hemolytic anemia
3. IgA deficiency with anti-IgA
4. Multiple red cell alloantibodies - ✔✔3. IgA deficiency with anti-IgA
✔✔The core (genome) of the HIV virion contains:
,1. DNA
2. RNA
3. Both RNA and DNA
4. Neither RNA or DNA - ✔✔2. RNA
✔✔Which of the following conditions can produce a normal aPTT and a prolonged PT?
1. Factor IX deficiency
2. Factor VII deficiency
3. Factor VIII deficiency
4. Factor X deficiency - ✔✔2. Factor VII deficiency
✔✔Identify the important blood cells displayed in this cerebrospinal fluid sample.
1. Atypical lymphocytes
2. Basophils
3. Monocytes
4. Blast cells - ✔✔4. Blast cells
✔✔VDRL and RPR are examples of which of the following types of test methodologies?
1. ELISA
2. Immunofluorescence
3. Flocculation
4. Hemagglutination - ✔✔3. Flocculation
✔✔If CSF tubes numbered #1, #2, and #3 CANNOT be analyzed within one hour, the
correct procedure for the microbiology sample tube is to:
1. Refrigerate it
2. Freeze it
3. Leave it at room temperature
4. Incubate in a 56-degree water bath - ✔✔3. Leave it at room temperature
✔✔What antibody is best described as common autoantibody that can be found in
virtually all sera?
1. Anti-I
2. Anti-K
3. Anti-Jk
4. Anti-M - ✔✔1. Anti-I
✔✔A false-positive result can be observed in a rheumatoid factor latex agglutination
procedure reaction due to:
, 1. Poor specimen quality
2. Antigen excess
3. Excessive complement levels
4. Juvenile idiopathic arthritis - ✔✔1. Poor specimen quality
✔✔The property MOST responsible for the migration of proteins in an electrical field is:
1. Molecular weight
2. Net surface charge
3. Endosmotic flow
4. Voltage - ✔✔2. Net surface charge
✔✔When giving a whole blood donation to a patient, what consideration must be made
in regards to the ABO/Rh compatibility between the recipient and the donor?
1. The recipient and the donor ABO or Rh compatibilities are not a concern
2. The recipient and donor ABO and Rh types must be compatible.
3. The recipient and donor ABO and Rh types must be identical.
4. The recipient and donor ABO types must be identical, but the Rh does not matter. -
✔✔3. The recipient and donor ABO and Rh types must be identical.
✔✔When three tubes of cerebrospinal fluid are received in the laboratory they should
be distributed to the various laboratory sections as follows:
1. #1 Hematology, #2 Chemistry, #3 Microbiology
2. #1 Chemistry, #2 Microbiology, #3 Hematology
3. #1 Microbiology, #2 Hematology, #3 Chemistry
4. #1 Chemistry, #2 Hematology, #3 Microbiology - ✔✔2. #1 Chemistry, #2
Microbiology, #3 Hematology
✔✔All of the following serological test results may occur in a patinet experiencing a
hemolytic transfusion reaction due to the presence of a clinically significant Rh antibody
such as anti-c EXCEPT:
1. Optimal reaction at 37 C or AHC phase
2. Preference to react with RBCs possessing a double-dose of Rh antigen
3. Binding complement
4. Positive DAT result - ✔✔3. Binding complement
✔✔What can be done to make a hapten behave like an antigen?
1. Denature the protein
2. Bind to protein
3. Bind to lipid
ANSWERS GRADED A+
✔✔Which of the following serological tests would be used for the diagnosis of Q-fever?
1. Wail-Felix test
2. Quellung test
3. EIA or indirect immunoflourescence
4. Cold agglutinin test - ✔✔3. EIA or indirect immunoflourescence
✔✔With most light microscopes, what is the total magnification if using the objective
lens that is closest to the specimen along with the ocular lens?
1. 100X
2. 200X
3. 400X
4. 1000X - ✔✔1. 100X
✔✔Which of the following methods may be employed to definitively identify Bence-
Jones proteins?
1. Immunoelectrophoresis
2. Sulfosalicyclic acid precipitation
3. Heat precipitation at 40-60 C
4. Urine dipstick - ✔✔1. Immunoelectrophoresis
✔✔Nephelometry is based on the principle of:
1. Turbidity resulting from specific antigen-coated latex particles agglutinated by
corresponding antibody
2. Agglutination resulting from physical attachment of antibody molecules to antigens on
an erythrocyte membrane.
3. Agglutination of host antibody to antigenic determinants on a bacterial agent
4. Precipitation of immune-related proteins in agarose gel - ✔✔1. Turbidity resulting
from specific antigen-coated latex particles agglutinated by corresponding antibody
✔✔Patients with which of the following conditions would benefit most from washed red
cells:
1. Warm autoimmune hemolytic anemia
2. Cold autoimmune hemolytic anemia
3. IgA deficiency with anti-IgA
4. Multiple red cell alloantibodies - ✔✔3. IgA deficiency with anti-IgA
✔✔The core (genome) of the HIV virion contains:
,1. DNA
2. RNA
3. Both RNA and DNA
4. Neither RNA or DNA - ✔✔2. RNA
✔✔Which of the following conditions can produce a normal aPTT and a prolonged PT?
1. Factor IX deficiency
2. Factor VII deficiency
3. Factor VIII deficiency
4. Factor X deficiency - ✔✔2. Factor VII deficiency
✔✔Identify the important blood cells displayed in this cerebrospinal fluid sample.
1. Atypical lymphocytes
2. Basophils
3. Monocytes
4. Blast cells - ✔✔4. Blast cells
✔✔VDRL and RPR are examples of which of the following types of test methodologies?
1. ELISA
2. Immunofluorescence
3. Flocculation
4. Hemagglutination - ✔✔3. Flocculation
✔✔If CSF tubes numbered #1, #2, and #3 CANNOT be analyzed within one hour, the
correct procedure for the microbiology sample tube is to:
1. Refrigerate it
2. Freeze it
3. Leave it at room temperature
4. Incubate in a 56-degree water bath - ✔✔3. Leave it at room temperature
✔✔What antibody is best described as common autoantibody that can be found in
virtually all sera?
1. Anti-I
2. Anti-K
3. Anti-Jk
4. Anti-M - ✔✔1. Anti-I
✔✔A false-positive result can be observed in a rheumatoid factor latex agglutination
procedure reaction due to:
, 1. Poor specimen quality
2. Antigen excess
3. Excessive complement levels
4. Juvenile idiopathic arthritis - ✔✔1. Poor specimen quality
✔✔The property MOST responsible for the migration of proteins in an electrical field is:
1. Molecular weight
2. Net surface charge
3. Endosmotic flow
4. Voltage - ✔✔2. Net surface charge
✔✔When giving a whole blood donation to a patient, what consideration must be made
in regards to the ABO/Rh compatibility between the recipient and the donor?
1. The recipient and the donor ABO or Rh compatibilities are not a concern
2. The recipient and donor ABO and Rh types must be compatible.
3. The recipient and donor ABO and Rh types must be identical.
4. The recipient and donor ABO types must be identical, but the Rh does not matter. -
✔✔3. The recipient and donor ABO and Rh types must be identical.
✔✔When three tubes of cerebrospinal fluid are received in the laboratory they should
be distributed to the various laboratory sections as follows:
1. #1 Hematology, #2 Chemistry, #3 Microbiology
2. #1 Chemistry, #2 Microbiology, #3 Hematology
3. #1 Microbiology, #2 Hematology, #3 Chemistry
4. #1 Chemistry, #2 Hematology, #3 Microbiology - ✔✔2. #1 Chemistry, #2
Microbiology, #3 Hematology
✔✔All of the following serological test results may occur in a patinet experiencing a
hemolytic transfusion reaction due to the presence of a clinically significant Rh antibody
such as anti-c EXCEPT:
1. Optimal reaction at 37 C or AHC phase
2. Preference to react with RBCs possessing a double-dose of Rh antigen
3. Binding complement
4. Positive DAT result - ✔✔3. Binding complement
✔✔What can be done to make a hapten behave like an antigen?
1. Denature the protein
2. Bind to protein
3. Bind to lipid
