Bio Lab 117 Midterm 167 correct
solutions Graded A+.
Bio Lab 117 Midterm 167 correct
solutions Graded A+.
What gets disposed in tip waste? - ANSWER gloves, sterile disposable pipets, microfuge tubes, blue and
yellow pipet tips
Where do sharp objects get disposed of? - ANSWER Razor blades go into the red Sharps Containers near
lab sinks.
Where does broken glass get disposed of? - ANSWER Report all broken glass to your TA. The waste will
go into the blue and white Broken Glass Boxes near the door.
Where does Ethidium Bromide waste get disposed of? - ANSWER All EtBr waster should go in the special
waste container.
Why is EtBr considered a mutagen? - ANSWER Because it is able to intercalate into DNA. Gloves should
be worn at all times when handling EtBr.
Where does chemical waste get disposed of? - ANSWER There are some chemicals you will use this
semester that need to be disposed of in a specific waste container. This waste container will be stored in
the hood.
Where is bacteria culture waste disposed of? - ANSWER It is harmful to dump this waster down the sink,
so you will remove all markings from your tubes and put this waste in a specific location. The LOCs will
put this waste into an autoclave before disposing of it.
What is an autoclave? - ANSWER An autoclave is a machine that sterilizes whatever is put into it with
high pressure, heat, an/or steam.
Where are bacteria plates and waste disposed of? - ANSWER The bacteria plates and any gloves that
were used to touch the bacteria plates should be thrown out in the medical waste bin.
,Bio Lab 117 Midterm 167 correct
solutions Graded A+.
How do you label a sample during lab? - ANSWER Add your section number, group number, your initials,
and a description of what the sample is.
Where do you label a petri dish? - ANSWER Always label the bottom of the dish because lids can be
switched.
How are documents labeled? - ANSWER Label documents on the computer with section number-group
number- first name last initial - assignment.
What is an assay? - ANSWER An assay is a tool used in science that helps to quantitatively measure the
amount of a single part of a total sample.
How does a LabQuest 2 and Vernier work? - ANSWER This system facilitates the collection of data by
replacing chemical techniques, providing real time graphical presentations, and saving information
directly in an electronic form.
What is Excel used for? - ANSWER Excel simplifies the identification and description of patterns or
relationships through its graphing and calculation functions. Computers and associated software
facilitate the collection and analysis of data so that patterns and relationships can be discovered,
describes, and verified.
What is the meter screen on the LabQuest used for? - ANSWER to measure discrete values, calibrate
probes, as well as set up collection parameters.
What is the graph screen on the LabQuest used for? - ANSWER to monitor data collection, as well as
determine simple statistical data information such as the average for a select data sample.
What is the table screen on the LabQuest used for? - ANSWER to see an overview of your collected data
values in table format.
What does accuracy mean? - ANSWER how close a measurement is to its true value.
, Bio Lab 117 Midterm 167 correct
solutions Graded A+.
What does precision mean? - ANSWER the ability to repeatedly measure a value in a fixed situation and
get the same results.
How do you keep pipet tips sterile? - ANSWER Throw them out after use; they are no longer sterile.
Once the pipet tip has touched the bench, your hand, or sleeve, it is no longer sterile and should be
thrown away.
What can affect the activity of proteins? - ANSWER The activity of proteins is very often dependent on
the pH, salt concentrations, and temperature of the reaction mixture.
What is a buffer? - ANSWER an aqueous solution containing a specific mixture of salts, buffering agents,
and sometimes reducing agents, detergents or cofactors, in which each of the components has a
purpose and is included to optimize the reaction. Buffers are extremely important in helping maintain
the pH of a solution.
What is stock solution? - ANSWER concentrated solutions that last over long periods of time. Such
concentrated stock solutions take up less space. In addition, these stocks are easily diluted for use when
necessary. Stock solutions are usually diluted with water.
What is the equation used to make a specific volume of dilute solution from a stock solution? - ANSWER
C1V1=C2V2
What is the dilution factor? - ANSWER the factor by which the concentration of the dilute solution is
reduced compared to the concentrate of the stock solution.
How do you calculate the dilution factor? - ANSWER DF = C1/C2 = V2/V1
What is a serial dilution? - ANSWER a stepwise dilution where the stock solution for each dilution in the
series is the dilute solution from the previous dilution.
What is the total dilution factor? - ANSWER the product of the dilution factors for each dilution step.
solutions Graded A+.
Bio Lab 117 Midterm 167 correct
solutions Graded A+.
What gets disposed in tip waste? - ANSWER gloves, sterile disposable pipets, microfuge tubes, blue and
yellow pipet tips
Where do sharp objects get disposed of? - ANSWER Razor blades go into the red Sharps Containers near
lab sinks.
Where does broken glass get disposed of? - ANSWER Report all broken glass to your TA. The waste will
go into the blue and white Broken Glass Boxes near the door.
Where does Ethidium Bromide waste get disposed of? - ANSWER All EtBr waster should go in the special
waste container.
Why is EtBr considered a mutagen? - ANSWER Because it is able to intercalate into DNA. Gloves should
be worn at all times when handling EtBr.
Where does chemical waste get disposed of? - ANSWER There are some chemicals you will use this
semester that need to be disposed of in a specific waste container. This waste container will be stored in
the hood.
Where is bacteria culture waste disposed of? - ANSWER It is harmful to dump this waster down the sink,
so you will remove all markings from your tubes and put this waste in a specific location. The LOCs will
put this waste into an autoclave before disposing of it.
What is an autoclave? - ANSWER An autoclave is a machine that sterilizes whatever is put into it with
high pressure, heat, an/or steam.
Where are bacteria plates and waste disposed of? - ANSWER The bacteria plates and any gloves that
were used to touch the bacteria plates should be thrown out in the medical waste bin.
,Bio Lab 117 Midterm 167 correct
solutions Graded A+.
How do you label a sample during lab? - ANSWER Add your section number, group number, your initials,
and a description of what the sample is.
Where do you label a petri dish? - ANSWER Always label the bottom of the dish because lids can be
switched.
How are documents labeled? - ANSWER Label documents on the computer with section number-group
number- first name last initial - assignment.
What is an assay? - ANSWER An assay is a tool used in science that helps to quantitatively measure the
amount of a single part of a total sample.
How does a LabQuest 2 and Vernier work? - ANSWER This system facilitates the collection of data by
replacing chemical techniques, providing real time graphical presentations, and saving information
directly in an electronic form.
What is Excel used for? - ANSWER Excel simplifies the identification and description of patterns or
relationships through its graphing and calculation functions. Computers and associated software
facilitate the collection and analysis of data so that patterns and relationships can be discovered,
describes, and verified.
What is the meter screen on the LabQuest used for? - ANSWER to measure discrete values, calibrate
probes, as well as set up collection parameters.
What is the graph screen on the LabQuest used for? - ANSWER to monitor data collection, as well as
determine simple statistical data information such as the average for a select data sample.
What is the table screen on the LabQuest used for? - ANSWER to see an overview of your collected data
values in table format.
What does accuracy mean? - ANSWER how close a measurement is to its true value.
, Bio Lab 117 Midterm 167 correct
solutions Graded A+.
What does precision mean? - ANSWER the ability to repeatedly measure a value in a fixed situation and
get the same results.
How do you keep pipet tips sterile? - ANSWER Throw them out after use; they are no longer sterile.
Once the pipet tip has touched the bench, your hand, or sleeve, it is no longer sterile and should be
thrown away.
What can affect the activity of proteins? - ANSWER The activity of proteins is very often dependent on
the pH, salt concentrations, and temperature of the reaction mixture.
What is a buffer? - ANSWER an aqueous solution containing a specific mixture of salts, buffering agents,
and sometimes reducing agents, detergents or cofactors, in which each of the components has a
purpose and is included to optimize the reaction. Buffers are extremely important in helping maintain
the pH of a solution.
What is stock solution? - ANSWER concentrated solutions that last over long periods of time. Such
concentrated stock solutions take up less space. In addition, these stocks are easily diluted for use when
necessary. Stock solutions are usually diluted with water.
What is the equation used to make a specific volume of dilute solution from a stock solution? - ANSWER
C1V1=C2V2
What is the dilution factor? - ANSWER the factor by which the concentration of the dilute solution is
reduced compared to the concentrate of the stock solution.
How do you calculate the dilution factor? - ANSWER DF = C1/C2 = V2/V1
What is a serial dilution? - ANSWER a stepwise dilution where the stock solution for each dilution in the
series is the dilute solution from the previous dilution.
What is the total dilution factor? - ANSWER the product of the dilution factors for each dilution step.
