MOLECULAR DIAGNOSTICS LAB
EXAM QUESTIONS AND ANSWERS
All are changes observed by scientists when studying a karyotype in order to
diagnose a genetic disease or diseases, EXCEPT.
A. TRANSLOCATION
B. DELETION
C. ISOMERIZATION
D. DUPLICATION - ANSWER-C
FILL IN THE BLANK SPACE. For prenatal diagnosis of genetic disorder
___________________________ specimens are best used as the source of cells for
karyotyping.
A. CSF
B. RED CELLS
C. SERUM
D. AMNIOTIC FLUID - ANSWER-D
In order to maximize the diagnostic information obtained from a chromosome
preparation, images of the individual chromosomes are arranged into a standardized
format known as a
A. Karyotype
B. Haplotype
C. Diplotype
D. All of these are correct - ANSWER-A
Human autosomes, or non-sex chromosomes, are numbered from
A. 1 to 22, in descending order by size
B. 1 to 22, in ascending order by size
C. 1 to 22, in any order order by size
D. 1 to 22, in mixed order by size - ANSWER-A
G-banding or ___________________________________banding is a technique
used in cytogenetics to produce a visible karyotype by staining condensed
chromosomes.
A. Giemsa banding
B. Grant banding
C. S-banding
D. R-banding - ANSWER-A
Aneuploidy is often caused by:
A. by the absence or addition of a chromosome
B. by the absence or addition of a amino acids
C. by the absence or addition of a nucleotide
D. none of these is correct - ANSWER-A
, In cytogenetics, Fluorescence in situ hybridization (FISH) and comparative genomic
hybridization (CGH) are NOT examples of two approaches that can potentially
identify abnormalities at the level of individual genes.
A. True
B. False - ANSWER-B
In order to prepare 100 mL 0.9% NaCl, you will need to weigh out 9g NaCl salt and
dissolve in 100 ml distilled water
A. True
B. False - ANSWER-B
The metaphase chromosomes are treated with trypsin to
A. partially digest the chromosome
B. strengthened the chromosomes
C. thickened the chromosomes
D. none of these is correct - ANSWER-A
Partial digestion of some chromosomal proteins relaxes the chromatin structure
thereby
A. allowing the Giemsa dye access to the DNA.
B. allowing the Giemsa dye repel DNA.
C. allowing the Giemsa dye repel RNA.
D. allowing the Giemsa dye access to the RNA. - ANSWER-A
Treatment with trypsin is done for how many minutes?
A. 2
B. 1
C. 4
D. 3 - ANSWER-A
When an alcohol is added to a solution of DNA, the DNA precipitates out of solution.
If there is enough DNA in the solution, what would you see?
A. yellow mass
B. clear solution
C. transparent mass
D. stringy white mass - ANSWER-D
What can extracted DNA NOT be used for?
A. Gene cloning
B. Genetic fingerprinting
C. Protein electrophoresis
D. Polymerase chain reaction - ANSWER-C
Which whole blood fraction is the most abundant source of genomic DNA?
A. Serum
B. Red blood cells
C. Buffy coat
EXAM QUESTIONS AND ANSWERS
All are changes observed by scientists when studying a karyotype in order to
diagnose a genetic disease or diseases, EXCEPT.
A. TRANSLOCATION
B. DELETION
C. ISOMERIZATION
D. DUPLICATION - ANSWER-C
FILL IN THE BLANK SPACE. For prenatal diagnosis of genetic disorder
___________________________ specimens are best used as the source of cells for
karyotyping.
A. CSF
B. RED CELLS
C. SERUM
D. AMNIOTIC FLUID - ANSWER-D
In order to maximize the diagnostic information obtained from a chromosome
preparation, images of the individual chromosomes are arranged into a standardized
format known as a
A. Karyotype
B. Haplotype
C. Diplotype
D. All of these are correct - ANSWER-A
Human autosomes, or non-sex chromosomes, are numbered from
A. 1 to 22, in descending order by size
B. 1 to 22, in ascending order by size
C. 1 to 22, in any order order by size
D. 1 to 22, in mixed order by size - ANSWER-A
G-banding or ___________________________________banding is a technique
used in cytogenetics to produce a visible karyotype by staining condensed
chromosomes.
A. Giemsa banding
B. Grant banding
C. S-banding
D. R-banding - ANSWER-A
Aneuploidy is often caused by:
A. by the absence or addition of a chromosome
B. by the absence or addition of a amino acids
C. by the absence or addition of a nucleotide
D. none of these is correct - ANSWER-A
, In cytogenetics, Fluorescence in situ hybridization (FISH) and comparative genomic
hybridization (CGH) are NOT examples of two approaches that can potentially
identify abnormalities at the level of individual genes.
A. True
B. False - ANSWER-B
In order to prepare 100 mL 0.9% NaCl, you will need to weigh out 9g NaCl salt and
dissolve in 100 ml distilled water
A. True
B. False - ANSWER-B
The metaphase chromosomes are treated with trypsin to
A. partially digest the chromosome
B. strengthened the chromosomes
C. thickened the chromosomes
D. none of these is correct - ANSWER-A
Partial digestion of some chromosomal proteins relaxes the chromatin structure
thereby
A. allowing the Giemsa dye access to the DNA.
B. allowing the Giemsa dye repel DNA.
C. allowing the Giemsa dye repel RNA.
D. allowing the Giemsa dye access to the RNA. - ANSWER-A
Treatment with trypsin is done for how many minutes?
A. 2
B. 1
C. 4
D. 3 - ANSWER-A
When an alcohol is added to a solution of DNA, the DNA precipitates out of solution.
If there is enough DNA in the solution, what would you see?
A. yellow mass
B. clear solution
C. transparent mass
D. stringy white mass - ANSWER-D
What can extracted DNA NOT be used for?
A. Gene cloning
B. Genetic fingerprinting
C. Protein electrophoresis
D. Polymerase chain reaction - ANSWER-C
Which whole blood fraction is the most abundant source of genomic DNA?
A. Serum
B. Red blood cells
C. Buffy coat
