UAMS MOLECULAR DIAGNOSTICS
EXAM 1 KAHOOT QUESTIONS AND
ANSWERS
Which scientists used x-ray diffraction to study the molecular structure of DNA?
Griffith & Miescher
Avery, MacLeod, McCarty
Rosalind Franklin & Maurice Wilkins
Watson & Crick - ANSWER-Rosalind Franklin & Maurice Wilkins
T/F: Cytosine, thymine, & uracil are pyrimidines. - ANSWER-True
A sugar + nitrogenous base is a ________.
Nucleotide polymer
Protein
Nucleotide
Nucleoside - ANSWER-Nucleoside
Nucleotide structure - ANSWER-phosphate, sugar, base
Which nitrogenous bases form 2 hydrogen bonds between them?
Guanine-Cytosine
Adenine-Thymine
Adenine-Cytosine
Guanine-Thymine - ANSWER-Adenine-Thymine
T/F: Nucleotide sequences in DNA or RNA are ALWAYS read in the 3' to 5' direction.
- ANSWER-False
What is the function of topoisomerase?
Glues Okazaki fragments together
Creates RNA primers
Unzips DNA
Prevents supercoiling - ANSWER-Prevents supercoiling
Choose all that apply: solid-phase nucleic acid extraction methods include which of
the following?
Phenol/chloroform method
Magnetic beads method
Silica spin column method
Liquid method - ANSWER-Magnetic beads method
Silica spin column method
, A DNA preparation had an OD260 of 0.64 and an OD280 of 0.41. Is this DNA
acceptable for testing? - ANSWER-no
What enzyme creates RNA primers during DNA replication?
DNA Polymerase III
DNA Polymerase I
Helicase
Primase - ANSWER-Primase
T/F: A restriction enzyme is an exonuclease that cuts DNA at specific sequences like
tiny molecular scissors. - ANSWER-False
Choose all that apply. Which of the following are true of transcription?
Occurs in the nucleus
Occurs in the cytoplasm
Results in mRNA strand
DNA is directly involved - ANSWER-Occurs in the nucleus
Results in mRNA strand
DNA is directly involved
What amino acid is coded for if the mRNA codon is CAU?
Histidine
Valine
Leucine
Methionine - ANSWER-Histidine
T/F: DNA exists as chromosomes in the nucleus most of the time. - ANSWER-False
The function of mRNA is _____________________. - ANSWER-Carries information
from DNA to ribosomes
Choose all that apply: Which are characteristics of RNA?
Easily degraded
Contains millions of nucleotides
Contains 10s-1000s of nucleotides
Pentose sugar is ribose - ANSWER-Easily degraded
Contains 10s-1000s of nucleotides
Pentose sugar is ribose
A DNA prep has an OD260 of 0.78 and an OD280 of 0.41. If it was diluted 1:5 and
TV = 100 uL, what's the original conc? - ANSWER-195 ug/mL
If an mRNA codon sequence is UGC, what is the tRNA anticodon?
ACG
EXAM 1 KAHOOT QUESTIONS AND
ANSWERS
Which scientists used x-ray diffraction to study the molecular structure of DNA?
Griffith & Miescher
Avery, MacLeod, McCarty
Rosalind Franklin & Maurice Wilkins
Watson & Crick - ANSWER-Rosalind Franklin & Maurice Wilkins
T/F: Cytosine, thymine, & uracil are pyrimidines. - ANSWER-True
A sugar + nitrogenous base is a ________.
Nucleotide polymer
Protein
Nucleotide
Nucleoside - ANSWER-Nucleoside
Nucleotide structure - ANSWER-phosphate, sugar, base
Which nitrogenous bases form 2 hydrogen bonds between them?
Guanine-Cytosine
Adenine-Thymine
Adenine-Cytosine
Guanine-Thymine - ANSWER-Adenine-Thymine
T/F: Nucleotide sequences in DNA or RNA are ALWAYS read in the 3' to 5' direction.
- ANSWER-False
What is the function of topoisomerase?
Glues Okazaki fragments together
Creates RNA primers
Unzips DNA
Prevents supercoiling - ANSWER-Prevents supercoiling
Choose all that apply: solid-phase nucleic acid extraction methods include which of
the following?
Phenol/chloroform method
Magnetic beads method
Silica spin column method
Liquid method - ANSWER-Magnetic beads method
Silica spin column method
, A DNA preparation had an OD260 of 0.64 and an OD280 of 0.41. Is this DNA
acceptable for testing? - ANSWER-no
What enzyme creates RNA primers during DNA replication?
DNA Polymerase III
DNA Polymerase I
Helicase
Primase - ANSWER-Primase
T/F: A restriction enzyme is an exonuclease that cuts DNA at specific sequences like
tiny molecular scissors. - ANSWER-False
Choose all that apply. Which of the following are true of transcription?
Occurs in the nucleus
Occurs in the cytoplasm
Results in mRNA strand
DNA is directly involved - ANSWER-Occurs in the nucleus
Results in mRNA strand
DNA is directly involved
What amino acid is coded for if the mRNA codon is CAU?
Histidine
Valine
Leucine
Methionine - ANSWER-Histidine
T/F: DNA exists as chromosomes in the nucleus most of the time. - ANSWER-False
The function of mRNA is _____________________. - ANSWER-Carries information
from DNA to ribosomes
Choose all that apply: Which are characteristics of RNA?
Easily degraded
Contains millions of nucleotides
Contains 10s-1000s of nucleotides
Pentose sugar is ribose - ANSWER-Easily degraded
Contains 10s-1000s of nucleotides
Pentose sugar is ribose
A DNA prep has an OD260 of 0.78 and an OD280 of 0.41. If it was diluted 1:5 and
TV = 100 uL, what's the original conc? - ANSWER-195 ug/mL
If an mRNA codon sequence is UGC, what is the tRNA anticodon?
ACG
