BB 450 Exam 2 questions and answers
graded A+ passed
Chymotrypsin: - correct answer ✔✔has an S1 pocket that determines specificity
of protease action
Attack by the alkoxide ion on the carbonyl carbon of the substrate of a serine
protease causes - correct answer ✔✔
With respect to serine proteases, - correct answer ✔✔a reaction intermediate is
stabilized in the oxyanion hole
The S1 pocket of serine proteases - correct answer ✔✔-is where the proper
substrate binds
-is near the catalytic triad
determines which amino acid side chain is bound
With respect to aspartyl proteases, - correct answer ✔✔they use an activated
hydroxyl made from water
With respect to restriction enzymes - correct answer ✔✔they act by cutting DNA
at specific nucleotide sequences
With respect to ATCase, - correct answer ✔✔
graded A+ passed
Chymotrypsin: - correct answer ✔✔has an S1 pocket that determines specificity
of protease action
Attack by the alkoxide ion on the carbonyl carbon of the substrate of a serine
protease causes - correct answer ✔✔
With respect to serine proteases, - correct answer ✔✔a reaction intermediate is
stabilized in the oxyanion hole
The S1 pocket of serine proteases - correct answer ✔✔-is where the proper
substrate binds
-is near the catalytic triad
determines which amino acid side chain is bound
With respect to aspartyl proteases, - correct answer ✔✔they use an activated
hydroxyl made from water
With respect to restriction enzymes - correct answer ✔✔they act by cutting DNA
at specific nucleotide sequences
With respect to ATCase, - correct answer ✔✔
