BB450 Unit 4: Glycolysis and
Glucogenesis questions well answered
graded A+
Fates of pyruvate from glycolysis - correct answer ✔✔Can feed into the citric acid
cycle or Fermentation
How much ATP is produced from Glycolysis - correct answer ✔✔2 ATP
How much ATP is produced with oxidative phosphorylation - correct answer
✔✔30-38 ATP yield
How much NADH do we get from glycolysis (per glucose)? - correct answer ✔✔2
What is the feedback inhibition of glycolysis? - correct answer ✔✔3 enzymes
Why do we depend more on breaking down glucose that fat when fat provides
more energy? - correct answer ✔✔Glucose is more readily available to break
down than fat. Can also be sent dissolved into the bloodstream really, really
quickly.
How is glucose stored in cells? - correct answer ✔✔Stored in polymers in the form
of glycogen in plants (amylose/amylopectin)
,1st rxn of glycolysis - correct answer ✔✔Glucose is converted to Glucose-6-
Phosphate by hexokinase taking a phosphate from ATP and places it on carbon 6
of glucose.
Enzyme involved in 1st rxn of glycolysis - correct answer ✔✔hexokinase
Rxn 1: Is energy spent? Is their regulation? - correct answer ✔✔Is an energetically
favorable reaction because we're using (spending) ATP. Hexokinase is regulated
because it is inhibited by its own product (glucose-6-phosphate). This prevents the
cell from overrunning glycolysis.
2nd rxn of glycolysis - correct answer ✔✔Glucose-6-phosphate gets rearranged to
frctose-6-phosphate.
Enzyme involved in 2nd rxn of glycolysis - correct answer
✔✔phosphoglucoisomerase
2nd rxn of glycolysis: Delta G 0 prime - correct answer ✔✔close to 0, so rxn is
readily reversible.
3rd rxn of glycolysis - correct answer ✔✔It puts a phosphate on position #1 of
fructose-6 phosphate, making it fructose-1,6-bisphosphate
Enzyme involved in 3rd reaction of glycolysis - correct answer
✔✔phosphofructokinase (PFK1)
, Does the third reaction require energy - correct answer ✔✔Requires ATP to make
it energetically favorable
How is the third reaction regulated? - correct answer ✔✔This enzyme is turned
on when cellular energy is low and turned off when cellular energy is high. AMP
and fructose-2,6-bisphosphate will activate the enzyme to do glycolysis.
What is up with ATP being able to be an inhibitor? - correct answer ✔✔ATP can be
an allosteric effector. This enzyme has 2 sides (one with high affinity for ATP and
one with low affinity for ATP). High affinity = substrate site, other = allosteric site.
How does ATP work as an allosteric effector? - correct answer ✔✔If there is a lot
or just enough it'll bind to the high-affinity site, but not the low-affinity site. If
there is a flood of ATP around, then the ATP will also find its way into the low-
affinity site, which will signal to the enzyme that because the low-affinity site is
full that there must be a lot of ATP, making it both inhibitory and a substrate.
4th rxn of glycolysis - correct answer ✔✔Uses aldolase to convert fructose-1,6-
bisphosphate to glyceraldehyde-3-Phosphate and dihydroxyacetone phosphate
(DHAP).
Enzyme involved in 4th rxn of glycolysis? - correct answer ✔✔Aldose
What do we go forward with after the 4th rxn of glycolysis? - correct answer
✔✔Makes the 6-carbon molecule break down into two three-carbon molecules.
Glucogenesis questions well answered
graded A+
Fates of pyruvate from glycolysis - correct answer ✔✔Can feed into the citric acid
cycle or Fermentation
How much ATP is produced from Glycolysis - correct answer ✔✔2 ATP
How much ATP is produced with oxidative phosphorylation - correct answer
✔✔30-38 ATP yield
How much NADH do we get from glycolysis (per glucose)? - correct answer ✔✔2
What is the feedback inhibition of glycolysis? - correct answer ✔✔3 enzymes
Why do we depend more on breaking down glucose that fat when fat provides
more energy? - correct answer ✔✔Glucose is more readily available to break
down than fat. Can also be sent dissolved into the bloodstream really, really
quickly.
How is glucose stored in cells? - correct answer ✔✔Stored in polymers in the form
of glycogen in plants (amylose/amylopectin)
,1st rxn of glycolysis - correct answer ✔✔Glucose is converted to Glucose-6-
Phosphate by hexokinase taking a phosphate from ATP and places it on carbon 6
of glucose.
Enzyme involved in 1st rxn of glycolysis - correct answer ✔✔hexokinase
Rxn 1: Is energy spent? Is their regulation? - correct answer ✔✔Is an energetically
favorable reaction because we're using (spending) ATP. Hexokinase is regulated
because it is inhibited by its own product (glucose-6-phosphate). This prevents the
cell from overrunning glycolysis.
2nd rxn of glycolysis - correct answer ✔✔Glucose-6-phosphate gets rearranged to
frctose-6-phosphate.
Enzyme involved in 2nd rxn of glycolysis - correct answer
✔✔phosphoglucoisomerase
2nd rxn of glycolysis: Delta G 0 prime - correct answer ✔✔close to 0, so rxn is
readily reversible.
3rd rxn of glycolysis - correct answer ✔✔It puts a phosphate on position #1 of
fructose-6 phosphate, making it fructose-1,6-bisphosphate
Enzyme involved in 3rd reaction of glycolysis - correct answer
✔✔phosphofructokinase (PFK1)
, Does the third reaction require energy - correct answer ✔✔Requires ATP to make
it energetically favorable
How is the third reaction regulated? - correct answer ✔✔This enzyme is turned
on when cellular energy is low and turned off when cellular energy is high. AMP
and fructose-2,6-bisphosphate will activate the enzyme to do glycolysis.
What is up with ATP being able to be an inhibitor? - correct answer ✔✔ATP can be
an allosteric effector. This enzyme has 2 sides (one with high affinity for ATP and
one with low affinity for ATP). High affinity = substrate site, other = allosteric site.
How does ATP work as an allosteric effector? - correct answer ✔✔If there is a lot
or just enough it'll bind to the high-affinity site, but not the low-affinity site. If
there is a flood of ATP around, then the ATP will also find its way into the low-
affinity site, which will signal to the enzyme that because the low-affinity site is
full that there must be a lot of ATP, making it both inhibitory and a substrate.
4th rxn of glycolysis - correct answer ✔✔Uses aldolase to convert fructose-1,6-
bisphosphate to glyceraldehyde-3-Phosphate and dihydroxyacetone phosphate
(DHAP).
Enzyme involved in 4th rxn of glycolysis? - correct answer ✔✔Aldose
What do we go forward with after the 4th rxn of glycolysis? - correct answer
✔✔Makes the 6-carbon molecule break down into two three-carbon molecules.
