BIOC 384 - SW 6 UPDATED ACTUAL
Exam Questions and CORRECT Answers
Which of the following amino acids is most likely to be found lining the hydrophobic substrate
channel of an enzyme? - CORRECT ANSWER - phenylalanine
Which of the following would you expect to be excluded from entering this hydrophobic
substrate channel? - CORRECT ANSWER - H2O and Ca2+
The maximum velocity - CORRECT ANSWER - A. is the velocity where addition of more
substrate does not cause a velocity increase.
Label the parts of the Michaelis-Menten graph. (Note: The pink line represents the enzyme
reaction with a high concentration of enzyme, while the green line represents the same enzyme
reaction with a lower enzyme concentration.) - CORRECT ANSWER - From Top to
Bottom on chart:
B(Vmax) --> C (1/2 Vmax) --> A (Km)
The active site of chymotrypsin is made up of - CORRECT ANSWER - Asp102 and His57
from the B chain and Ser195 from the C chain.
Which of the following biochemical concepts does the animation best illustrate? - CORRECT
ANSWER - The three-dimensional final folded structure of enzymes is critical for the
formation of catalytic sites that may not be predicted solely by primary sequence alone.
Label the following figure. (Not all labels may be used.) Remember that an enzyme's active site
binds to substrate based on the shape of both the enzyme and active site, as well as the attraction
between amino acid residues (or cofactors) in the active site and on the substrate. For example,
opposite charges will attract one another, and nonpolar residues will attract one another. -
CORRECT ANSWER - - A (Hydrophobic regions) and D (Charged residues) ON TOP
HALF
- B (Enzyme-substrate complex with induced-fit binding to substrate) ON BOTTOM HALF
Exam Questions and CORRECT Answers
Which of the following amino acids is most likely to be found lining the hydrophobic substrate
channel of an enzyme? - CORRECT ANSWER - phenylalanine
Which of the following would you expect to be excluded from entering this hydrophobic
substrate channel? - CORRECT ANSWER - H2O and Ca2+
The maximum velocity - CORRECT ANSWER - A. is the velocity where addition of more
substrate does not cause a velocity increase.
Label the parts of the Michaelis-Menten graph. (Note: The pink line represents the enzyme
reaction with a high concentration of enzyme, while the green line represents the same enzyme
reaction with a lower enzyme concentration.) - CORRECT ANSWER - From Top to
Bottom on chart:
B(Vmax) --> C (1/2 Vmax) --> A (Km)
The active site of chymotrypsin is made up of - CORRECT ANSWER - Asp102 and His57
from the B chain and Ser195 from the C chain.
Which of the following biochemical concepts does the animation best illustrate? - CORRECT
ANSWER - The three-dimensional final folded structure of enzymes is critical for the
formation of catalytic sites that may not be predicted solely by primary sequence alone.
Label the following figure. (Not all labels may be used.) Remember that an enzyme's active site
binds to substrate based on the shape of both the enzyme and active site, as well as the attraction
between amino acid residues (or cofactors) in the active site and on the substrate. For example,
opposite charges will attract one another, and nonpolar residues will attract one another. -
CORRECT ANSWER - - A (Hydrophobic regions) and D (Charged residues) ON TOP
HALF
- B (Enzyme-substrate complex with induced-fit binding to substrate) ON BOTTOM HALF
