CSN BIO 251 LAB - FINAL
Optimum - answerThe temperature at which the organism grows best over time is
___________________.
Minimum - answerThe temperature, below which the organism cannot grow is called the
__________________.
Maximum - answerThe temperature, above which the organism cannot grow is called
the _________________.
Turbidity - answerThe optimum temperature of growth may be observed in broth
cultures as measures of _____________________.
absorbance - answerThe turbidity is measured by ___________________.
spectrophotometer - answerWhat tool is used to measure the turbidity?
proportional - answerThe higher the absorbance of light, the higher the growth, i.e. they
are ______________________.
Optical Density - answerAs cells grow, more light is absorbed, and the higher the
absorbance reading, this is also referred to as what?
Psychrophiles - answerOrganisms that grow only below 20 degrees C are classified as
_________________________.
Psychrotrophs - answerOrganisms that live in the cold but can also grow up to 35
degrees C and slightly higher, are classified as _____________________.
Mesophiles - answerMicrobes that are adapted to temperatures between 15 degrees C
and 45 degrees C are classified as ________________________.
thermophiles - answerOrganisms growing above 45 degrees C - 80 degrees C are
classified as _________________________.
extream thermophiles - answerMicrobes growing above 80 degrees C up to 110
degrees C are ___________________ _____________________.
thermoduric - answerThese bacteria generally are mesophiles that endure high
temperatures for short periods of time.
, E. coli and Bacillus stearothermophilus - answerWhat two bacterial cultures were used
for the Growth Factors Temperature Lab?
4, 25, 36, 55 - answerWhat were the four temperatures the bacteria were kept at for the
Growth Factors Temperature lab?
absorbance - answerThe objective of the Growth Factors Temperature Lab is to
quantify the amount of turbidity and therefore the amount of bacterial growth by
obtaining an _______________________ value for each nutrient broth.
550 nm - answerWhat is the wavelength used to measure the absorbance of the
bacteria of the Growth Factors Temperature Lab?
water activity - answerThe availability of water is called the _______________
________________, and values vary between 0 and 1.0.
Osmosis - answer____________________, the movement of water across a
membrane, occurs as a result of the different solute concentrations on either side of the
membrane.
isotonic - answerIf the solute concentrations are equal or similar on either side of the
membrane, it is an ______________________ environment and water does not move
favorably in either direction.
hypotonic - answerIf the environment has very few solutes compared to the cytoplasm,
this is called ___________________.
Osmotic Pressure - answerBecause water moves toward the higher solute
concentration, water will move into the cell due to ______________
___________________.
Swell - answerBacterial cells with cell walls will ______________ and have increased
turgor pressure.
Hypertonic - answer__________________ environments are those in which the solute
concentration is higher in the environment as compared to the cytoplasm.
plasmolysis - answerAs the cell loses water from its cytoplasm the cell membrane
shrinks away from the cell wall, this process is called ________________________.
Halophiles - answer_____________________ are adapted to live and grow only in high
salinity.
extream halophiles - answer_______________ _______________ can only grow in
15%-30% saline environment.
Optimum - answerThe temperature at which the organism grows best over time is
___________________.
Minimum - answerThe temperature, below which the organism cannot grow is called the
__________________.
Maximum - answerThe temperature, above which the organism cannot grow is called
the _________________.
Turbidity - answerThe optimum temperature of growth may be observed in broth
cultures as measures of _____________________.
absorbance - answerThe turbidity is measured by ___________________.
spectrophotometer - answerWhat tool is used to measure the turbidity?
proportional - answerThe higher the absorbance of light, the higher the growth, i.e. they
are ______________________.
Optical Density - answerAs cells grow, more light is absorbed, and the higher the
absorbance reading, this is also referred to as what?
Psychrophiles - answerOrganisms that grow only below 20 degrees C are classified as
_________________________.
Psychrotrophs - answerOrganisms that live in the cold but can also grow up to 35
degrees C and slightly higher, are classified as _____________________.
Mesophiles - answerMicrobes that are adapted to temperatures between 15 degrees C
and 45 degrees C are classified as ________________________.
thermophiles - answerOrganisms growing above 45 degrees C - 80 degrees C are
classified as _________________________.
extream thermophiles - answerMicrobes growing above 80 degrees C up to 110
degrees C are ___________________ _____________________.
thermoduric - answerThese bacteria generally are mesophiles that endure high
temperatures for short periods of time.
, E. coli and Bacillus stearothermophilus - answerWhat two bacterial cultures were used
for the Growth Factors Temperature Lab?
4, 25, 36, 55 - answerWhat were the four temperatures the bacteria were kept at for the
Growth Factors Temperature lab?
absorbance - answerThe objective of the Growth Factors Temperature Lab is to
quantify the amount of turbidity and therefore the amount of bacterial growth by
obtaining an _______________________ value for each nutrient broth.
550 nm - answerWhat is the wavelength used to measure the absorbance of the
bacteria of the Growth Factors Temperature Lab?
water activity - answerThe availability of water is called the _______________
________________, and values vary between 0 and 1.0.
Osmosis - answer____________________, the movement of water across a
membrane, occurs as a result of the different solute concentrations on either side of the
membrane.
isotonic - answerIf the solute concentrations are equal or similar on either side of the
membrane, it is an ______________________ environment and water does not move
favorably in either direction.
hypotonic - answerIf the environment has very few solutes compared to the cytoplasm,
this is called ___________________.
Osmotic Pressure - answerBecause water moves toward the higher solute
concentration, water will move into the cell due to ______________
___________________.
Swell - answerBacterial cells with cell walls will ______________ and have increased
turgor pressure.
Hypertonic - answer__________________ environments are those in which the solute
concentration is higher in the environment as compared to the cytoplasm.
plasmolysis - answerAs the cell loses water from its cytoplasm the cell membrane
shrinks away from the cell wall, this process is called ________________________.
Halophiles - answer_____________________ are adapted to live and grow only in high
salinity.
extream halophiles - answer_______________ _______________ can only grow in
15%-30% saline environment.
