Glycolysis - Biochem 301 Exam
2a with 100% correct answers
(graded A+)
glycerol - answer What is this?
glyceraldehyde - answer What is this?
glyceraldehyde 3-phosphate - answer What is this?
catabolic pathways - answer complex substances are degraded to
smaller molecules
breaking down, get E out
anabolic pathways - answer complex substances are synthesized
from simpler molecules
building bigger things, consumes E (we must put it in)
It breaks down glucose and generates important intermediates
both an anabolic and catabolic pathway - answer What is the
general purpose of glycolysis?
glucose => fructose-1,6-biphosphate (two triose phosphates) => 2
pyruvate
First five reactions constitute the energy investment phase
,⎻ sugar phosphates are synthesized at the expense of two ATPs
⎻ six-carbon substrate is split into two three-carbon sugar
phosphates
Last five reactions constitute the energy generation phase
⎻ two triose phosphates are converted to energy-rich compounds -
answer Explain what happens during glycolysis from a forest
perspective? (What are the two phases, what is the initial product,
intermediate between phases, and final product)
Glucose => 2 pyruvate
2 ADP => 2 ATP
2 NAD+ => 2 NADH - answer What are the net products of
glycolysis?
10
1-5 are energy-investment
6-10 are energy generation - answer How many total steps are there
in glycolysis?
pyruvate
two of these are the final products of glycolysis - answer What is
this? What is important about it?
No - answer Does glycolysis require O2?
In the cytoplasm of cells - answer Where does glycolysis take place?
, glucose is phosphorylated to give glucose-6-phosphate
(add a Phosphate group to the 6th carbon)
ATP input => ADP results
Hexokinase - answer What happens in the 1st step of glycolysis?
What *enzyme catalyzes the rxn?
isomerization of glucose-6-phosphate (a-D)
this results in fructose-6-phosphate (D)
reaction is catalyzed by glucose-6-phosphate isomerase
this step sets up the sugar for a symmetric cleavage - answer What
happens in 2nd step of glycolysis? Enzyme?
D-fructose-6-phosphate + ATP => D-fructose-1,6-biphosphate + ADP
+ H+
second ATP is invested
Enzyme = phosphofructokinase (PFK represents primary site for
regulation of glycolysis) - answer What happens in the 3rd step of
glycolysis? *Enzyme?
cleavage of D-fructose-1,6-biphosphate to two triose phosphates
(six carbon sugar (pentose ring) broken down into two 3 carbon
molecules)
products are:
1) dihydroxyacetone phosphate (DHAP)
2) D-glyceraldehyde-3-phosphate (GAP)
2a with 100% correct answers
(graded A+)
glycerol - answer What is this?
glyceraldehyde - answer What is this?
glyceraldehyde 3-phosphate - answer What is this?
catabolic pathways - answer complex substances are degraded to
smaller molecules
breaking down, get E out
anabolic pathways - answer complex substances are synthesized
from simpler molecules
building bigger things, consumes E (we must put it in)
It breaks down glucose and generates important intermediates
both an anabolic and catabolic pathway - answer What is the
general purpose of glycolysis?
glucose => fructose-1,6-biphosphate (two triose phosphates) => 2
pyruvate
First five reactions constitute the energy investment phase
,⎻ sugar phosphates are synthesized at the expense of two ATPs
⎻ six-carbon substrate is split into two three-carbon sugar
phosphates
Last five reactions constitute the energy generation phase
⎻ two triose phosphates are converted to energy-rich compounds -
answer Explain what happens during glycolysis from a forest
perspective? (What are the two phases, what is the initial product,
intermediate between phases, and final product)
Glucose => 2 pyruvate
2 ADP => 2 ATP
2 NAD+ => 2 NADH - answer What are the net products of
glycolysis?
10
1-5 are energy-investment
6-10 are energy generation - answer How many total steps are there
in glycolysis?
pyruvate
two of these are the final products of glycolysis - answer What is
this? What is important about it?
No - answer Does glycolysis require O2?
In the cytoplasm of cells - answer Where does glycolysis take place?
, glucose is phosphorylated to give glucose-6-phosphate
(add a Phosphate group to the 6th carbon)
ATP input => ADP results
Hexokinase - answer What happens in the 1st step of glycolysis?
What *enzyme catalyzes the rxn?
isomerization of glucose-6-phosphate (a-D)
this results in fructose-6-phosphate (D)
reaction is catalyzed by glucose-6-phosphate isomerase
this step sets up the sugar for a symmetric cleavage - answer What
happens in 2nd step of glycolysis? Enzyme?
D-fructose-6-phosphate + ATP => D-fructose-1,6-biphosphate + ADP
+ H+
second ATP is invested
Enzyme = phosphofructokinase (PFK represents primary site for
regulation of glycolysis) - answer What happens in the 3rd step of
glycolysis? *Enzyme?
cleavage of D-fructose-1,6-biphosphate to two triose phosphates
(six carbon sugar (pentose ring) broken down into two 3 carbon
molecules)
products are:
1) dihydroxyacetone phosphate (DHAP)
2) D-glyceraldehyde-3-phosphate (GAP)
