Dev Animal Bio 4400 Exam 1 With Complete Solutions!!
fertilization, cleavage, gastrulation, organogenesis, hatching
(animals) list the stages of development
false
animal development is not conserved (T/F)
true
the time for development to differs among species (T/F)
find it (track where cells go), move it (transplant cells), lose/gain it (remove gene
products
what are the 3 keys to developmental biology?
in situ hybridization, RNA-seq, QPCR
list some techniques that are used to track cells (RNA)
Immunohistochemistry
list some techniques that are used to track cells (protein)
Immunohistochemistry
marking a protein in a cell is known as what type of technique?
in situ hybridization
marking a specific RNA in its original place in an embryo is known as what type of
technique?
,RNA sequencing
a higher-throughput technique used to analyze and quantify a complete set of RNA
molecules in a biological sample
general
what type of approach is RNA sequencing (general/specific)?
qPCR
a sensitive laboratory technique used to amplify and quantify specific DNA/RNA
sequences in real-time
specific
what type of approach is quantitative PCR (general/specific)?
single cell RNA seq
this type of RNA sequencing uses a complete genomic profile at a single cell level.
identification is based on clusters of marker genes
spatial RNA sequencing
this type of RNA sequencing profiles the embryo in situ -- meaning that it maintains
spatial organization of tissue
transplantation (graft of cells), injection of mRNA, implantation of bead soaked in
protein
list some techniques that transplant cells (move it) ?
donor
fluorescent markers track the fate of ____ cells and can show how they integrate into
,things such as neural circuits
cell fate
using green fluorescence (GFAP), what can be determined?
tells us cell fate
what is the importance of donor cells in the move it key of developmental bio?
find out if transplanted cells keep their OG development fate
what is the purpose of the move it (transplantation) process
acceptor
if transplanted cells change their fate, it suggest that the ___ embryo provides
instructive signals
removal of cells, mutagenesis, CRISPR-Cas9, Injection or implantation of competitor
some techniques that remove cells (lose/gain it) are:
(removal of cells/hybridization
mutagenesis/ qPCR
CRISPR-Cas9/ RNA seq.
Injection or implantation of competitor/ immunohistochemistry)
how their absence impacts embryo
, if you remove a cell from the embryo, what can you watch for?
gene function
if you use mutagenesis in the lose it stage developmental bio, what can you track ?
how gene functions in competition with another gene
if you inject, transplsnt or implant a competitor during the lose it phase, what can you
see?
CRISPR-Cas9
a revolutionary genome-editing tool that uses a bacterial RNA-guided enzyme to
precisely cut DNA at specific locations, enabling targeted genetic modifications
true
development is a dynamic process (T/F)
true
the fundamental rules from development are (T/F):
- timing
-locations
- cell-cell interactions
- context
local changes
germ layers
BMP (bone morphogenetic protein) signaling in zebrafish is important for the formation
of ____ ___
fertilization, cleavage, gastrulation, organogenesis, hatching
(animals) list the stages of development
false
animal development is not conserved (T/F)
true
the time for development to differs among species (T/F)
find it (track where cells go), move it (transplant cells), lose/gain it (remove gene
products
what are the 3 keys to developmental biology?
in situ hybridization, RNA-seq, QPCR
list some techniques that are used to track cells (RNA)
Immunohistochemistry
list some techniques that are used to track cells (protein)
Immunohistochemistry
marking a protein in a cell is known as what type of technique?
in situ hybridization
marking a specific RNA in its original place in an embryo is known as what type of
technique?
,RNA sequencing
a higher-throughput technique used to analyze and quantify a complete set of RNA
molecules in a biological sample
general
what type of approach is RNA sequencing (general/specific)?
qPCR
a sensitive laboratory technique used to amplify and quantify specific DNA/RNA
sequences in real-time
specific
what type of approach is quantitative PCR (general/specific)?
single cell RNA seq
this type of RNA sequencing uses a complete genomic profile at a single cell level.
identification is based on clusters of marker genes
spatial RNA sequencing
this type of RNA sequencing profiles the embryo in situ -- meaning that it maintains
spatial organization of tissue
transplantation (graft of cells), injection of mRNA, implantation of bead soaked in
protein
list some techniques that transplant cells (move it) ?
donor
fluorescent markers track the fate of ____ cells and can show how they integrate into
,things such as neural circuits
cell fate
using green fluorescence (GFAP), what can be determined?
tells us cell fate
what is the importance of donor cells in the move it key of developmental bio?
find out if transplanted cells keep their OG development fate
what is the purpose of the move it (transplantation) process
acceptor
if transplanted cells change their fate, it suggest that the ___ embryo provides
instructive signals
removal of cells, mutagenesis, CRISPR-Cas9, Injection or implantation of competitor
some techniques that remove cells (lose/gain it) are:
(removal of cells/hybridization
mutagenesis/ qPCR
CRISPR-Cas9/ RNA seq.
Injection or implantation of competitor/ immunohistochemistry)
how their absence impacts embryo
, if you remove a cell from the embryo, what can you watch for?
gene function
if you use mutagenesis in the lose it stage developmental bio, what can you track ?
how gene functions in competition with another gene
if you inject, transplsnt or implant a competitor during the lose it phase, what can you
see?
CRISPR-Cas9
a revolutionary genome-editing tool that uses a bacterial RNA-guided enzyme to
precisely cut DNA at specific locations, enabling targeted genetic modifications
true
development is a dynamic process (T/F)
true
the fundamental rules from development are (T/F):
- timing
-locations
- cell-cell interactions
- context
local changes
germ layers
BMP (bone morphogenetic protein) signaling in zebrafish is important for the formation
of ____ ___
