CORRECT DETAILED ANSWERS
A donor is tested for viral markers and the intial EIA screening test for anti-HIV-1/2 is
reactive. Which choice below represents the next step that is taken? - Answer-Repeat
the EIA test in duplicate
Which of the following is used as a confirmatory test when the HIV ELISA assay is
repeat reactive? - Answer-Western Blot
The rationale for deglycerolizing frozen RBCs with extensive washing is - Answer-
Glycerol can cause hemolysis
Positive with Glycine soja - Answer-T, Tn, Cad
Positive with Arachi hypogaea - Answer-T, Th, Tk
Positive with Dolichos biflorus - Answer-Tn and Cad
Positive with Salvia sclarea - Answer-Tn
Positive with Salvia horminum - Answer-Tn and Cad
Positive with Griffonia simplicifolia - Answer-Tk
Positive with cord serum - Answer-none
Positive Ham's Test - Answer-HEMPAS
Neg Ficin/papain
Neg Trypsin
Neg alpha chy
Pos 0.2M DTT - Answer-Bpa, Ch/Rg, Xg
Neg Ficin/papain
Neg Trypsin
Neg alpha chy
Neg 0.2M DTT - Answer-IN, JMH
Neg Ficin/papain
Neg Trypsin
Pos alpha chy
Pos 0.2M DTT - Answer-M, N, EnaTs, Ge2, Ge4
,Neg Ficin/papain
Pos Trypsin
Neg alpha chy
Pos 0.2M DTT - Answer-'N', Fya, Fyb
Variable Ficin/papain
Pos Trypsin
Neg alpha chy
Pos 0.2M DTT - Answer-S, s
Variable Ficin/papain
Pos Trypsin
Neg alpha chy
Weak/ Neg 0.2M DTT - Answer-YT
Neg Ficin/papain
Pos Trypsin
Pos alpha chy
Pos 0.2M DTT - Answer-EnaFS
Pos Ficin/papain
Neg Trypsin
Neg alpha chy
Weak/Neg 0.2M DTT - Answer-LU, MER2
Weak orNeg Ficin
Positive papain
Neg Trypsin
Neg alpha chy
Neg 0.2M DTT - Answer-KN
Pos Ficin/papain
Neg Trypsin
Weak alpha chy
Neg 0.2M DTT - Answer-DO
Pos Ficin/papain
Pos Trypsin
Neg alpha chy
Weak 0.2M DTT - Answer-CROM
Pos Ficin/papain
Pos Trypsin
Neg alpha chy
Pos 0.2M DTT - Answer-Some Di
, Pos Ficin/papain
Pos Trypsin
Pos/weak alpha chy
Neg 0.2M DTT - Answer-LW
Pos Ficin/papain
Pos/weak Trypsin
Pos/weak alpha chy
Pos 0.2M DTT - Answer-SC
Pos Ficin/papain
Pos Trypsin
Pos alpha chy
Neg 0.2M DTT - Answer-Kel (except KALT which is trypsin sensitive)
Pos Ficin/papain
Pos Trypsin
Pos alpha chy
Pos 0.2M DTT - Answer-ABO, EnaFr, U, P1PK, RH, LE, FY3, JK, Most DI, CO, H, Ge3,
OK, I/I, P, FORS, JR, LAN, Csa, ER, LKE, PX2, Vel, ABTI, Ata, EMM, AnWj, Sda, Pel,
MAM
Pos Ficin/papain
Pos Trypsin
Pos alpha chy
Enhanced 0.2M DTT - Answer-Kx
K0 Phenotype (antigens) - Answer-Kx, No Kell, Ku-, Km-
K0 Phenotype (antibody) - Answer-anti-Ku, Anti-Km
K0 Phenotype( compatible blood) - Answer-K0 (anti-Ku)
K0 and McLeod (anti-Km)
Non-CGD McLeod Phenotype (males) (antigens) - Answer-No Kx, weak Kell ags
Non-CGD McLeod Phenotype (males) antibody - Answer-anti-Km, anti-Kx(only 1
example)
Non-CGD McLeod Phenotype (males) compatible blood - Answer-anti-Km (K0 and
McLeod), Anti-Kx (McLeod)
McLeod Phenotype with CGD males (antigens) - Answer-No Kx, weak Kell ags
Mcleod phenotype with CGD males - Answer-anti-Kx plus anti-Km