ASCP recalls (Updated)
Questions & Answers
Verified 100% Correct!!
100 true positives & 100/100 true negatives. Procedure #2 detected 80/100 true
positives and 70/100 true negatives. - ANS Procedure 2 is more sensitive (refers to
% of true positives that are accurately identified as positives). Specificity refers to # of
true negatives identified as negative.
TP/TP+FN= - ANS Sensitivity
TN/TN+FP= - ANS Specificity
What might the following indicate in urine: RBCs, WBCs, nitrite, and bacteria? - ANS
Pyelonephritis (UTI); WBCs and nitrites in urine are classical indication of bacterial
infection (UTI)
Why is albumin the first protein to be detected in tests for renal failure? - ANS It's
molecular size is the smallest
Cortisol excess will result in: - ANS Elevated glucose in blood; cortisol counteracts
insulin
What is the reason for the following discrepency?
Anti A: 3+
Anti B: 3+
A1: 3+
B cells: 0 - ANS Patient may be A2B
This spiral form organism is seen in urine and cultured on fletcher's medium - ANS
Leptospira
Organism that gives off a bleach-like odor in culture: - ANS Eikenella (GN pits agar)
,Presence of Rheumatoid factor in blood may result in false positives for what test? -
ANS VDRL; nonspecific screening test for syphillis
Disease associated with the following: increased TSH increased T3 and increased FT4:
- ANS Pituitary tumor
If excess PTH is released, what woulde you find elevated amounts of in serum? - ANS
Calcium
Mucoid pink colonies on plate that produce gase, indole positive, yellow/yellow on TSI
- ANS Klebsiella oxytoca (Klebsiella pneumo is indole negative)
PAD positive, indole positive, organism stains gram negative. What is it? - ANS
Proteus vulgaris (p. mirabilis is indole negative)
Curved gram negative bacilli. Cultured form GI tract of persons with ulcers. What test
would you do to confirm its identity? - ANS Test for Urease. Most likely H. pylori
which is curved, infects GI tract, and the urease activity can cause ulcers.
Enzyme controls run on a machine gives result around -3SD. Samples run on the same
machine give results <1 SD. What is the problem? - ANS Controls were left @
room temperature for several days.
HIV-1 and HIV-2 combination ELISA test is positive in a patient with symptoms of
immune deficiency. Western blot was inconclusive for HIV-1. What do you do next? -
ANS DO HIV-2 western blot.
What are steps of PCR? - ANS Denaturation, annealing, and transcription.
RAST test detects what? - ANS IgE to particular antigens (RIST is for total IgE)
After collecting a blood sample in an EDTA tube for CBC you find that the Hct is very
high (68%). What should you do next? - ANS Report results. Always use EDTA for
CBC specimens.
When you conduct a procedure using fluorescence it is important to protect yourself
from: - ANS Exciting light; high energy light produced by the machine
Blood was collected Nov. 1st then frozen with glycol on Nov. 5th. What is the new
expiration date? - ANS Nov. 1 10 years from now.
, A person was successfully treated for syphillis 12 years ago. he just came in worried
about being reinfected. what would you look for in his blood? - ANS VDRL; TPA is
not correct because it can remain active for the life of the patient.
Patient demonstrates positive antibody screen. You suspect either Jk(a), K or c. You
know from previous history that the patient has Jk(a) antigen on their red cells. You
react the patients serum with cells positive for certain antigens and see: K cells: 0, c
cells: 4+. What can be concluded - ANS You can rule out c antigen but cannot
confirm absence or presence of K antigen on patients red cells. Patient producing anti-c
and therefore does not have the c antigen. K did not react so patient either has K
antigen or has never been exposed to K and therefore does not make antibodies to K.
Urine protein chemistry dipstick detected no proteins by sulfosalicylic acid (SSA) test did
detect proteins. Why? - ANS Bence-jones proteins in urine
Several immature granulocytes in PBS. What stain should be used to figure this
persons problem out? - ANS LAP
Syndrome of inappropriate antidiuretic hormone secretions (SIADH) would result in
what in blood? - ANS Deficient in sodium
Fiber strands in urine resemble what under the microscope? - ANS Hyaline casts
Which of the following regulates myocyte contraction - ANS Cardiac troponins
HBA1C levels cannot always be used to monitor glucose levels in conditions such as:
- ANS Sickle cell disease; any disorder that causes RBCs to die prematurely (sickle
cell, g6pd etc) will result in underestimation of HBA1C levels
Strentrophomonas: - ANS Rapid oxidizers of maltose
Burr cells indicative of - ANS Uremia
Stain used for Cryptosporidium parvum - ANS Modified trichrome stain
Stomatocytes indicative of - ANS Liver disease
Acanthocytes: - ANS Inadequate drying
Questions & Answers
Verified 100% Correct!!
100 true positives & 100/100 true negatives. Procedure #2 detected 80/100 true
positives and 70/100 true negatives. - ANS Procedure 2 is more sensitive (refers to
% of true positives that are accurately identified as positives). Specificity refers to # of
true negatives identified as negative.
TP/TP+FN= - ANS Sensitivity
TN/TN+FP= - ANS Specificity
What might the following indicate in urine: RBCs, WBCs, nitrite, and bacteria? - ANS
Pyelonephritis (UTI); WBCs and nitrites in urine are classical indication of bacterial
infection (UTI)
Why is albumin the first protein to be detected in tests for renal failure? - ANS It's
molecular size is the smallest
Cortisol excess will result in: - ANS Elevated glucose in blood; cortisol counteracts
insulin
What is the reason for the following discrepency?
Anti A: 3+
Anti B: 3+
A1: 3+
B cells: 0 - ANS Patient may be A2B
This spiral form organism is seen in urine and cultured on fletcher's medium - ANS
Leptospira
Organism that gives off a bleach-like odor in culture: - ANS Eikenella (GN pits agar)
,Presence of Rheumatoid factor in blood may result in false positives for what test? -
ANS VDRL; nonspecific screening test for syphillis
Disease associated with the following: increased TSH increased T3 and increased FT4:
- ANS Pituitary tumor
If excess PTH is released, what woulde you find elevated amounts of in serum? - ANS
Calcium
Mucoid pink colonies on plate that produce gase, indole positive, yellow/yellow on TSI
- ANS Klebsiella oxytoca (Klebsiella pneumo is indole negative)
PAD positive, indole positive, organism stains gram negative. What is it? - ANS
Proteus vulgaris (p. mirabilis is indole negative)
Curved gram negative bacilli. Cultured form GI tract of persons with ulcers. What test
would you do to confirm its identity? - ANS Test for Urease. Most likely H. pylori
which is curved, infects GI tract, and the urease activity can cause ulcers.
Enzyme controls run on a machine gives result around -3SD. Samples run on the same
machine give results <1 SD. What is the problem? - ANS Controls were left @
room temperature for several days.
HIV-1 and HIV-2 combination ELISA test is positive in a patient with symptoms of
immune deficiency. Western blot was inconclusive for HIV-1. What do you do next? -
ANS DO HIV-2 western blot.
What are steps of PCR? - ANS Denaturation, annealing, and transcription.
RAST test detects what? - ANS IgE to particular antigens (RIST is for total IgE)
After collecting a blood sample in an EDTA tube for CBC you find that the Hct is very
high (68%). What should you do next? - ANS Report results. Always use EDTA for
CBC specimens.
When you conduct a procedure using fluorescence it is important to protect yourself
from: - ANS Exciting light; high energy light produced by the machine
Blood was collected Nov. 1st then frozen with glycol on Nov. 5th. What is the new
expiration date? - ANS Nov. 1 10 years from now.
, A person was successfully treated for syphillis 12 years ago. he just came in worried
about being reinfected. what would you look for in his blood? - ANS VDRL; TPA is
not correct because it can remain active for the life of the patient.
Patient demonstrates positive antibody screen. You suspect either Jk(a), K or c. You
know from previous history that the patient has Jk(a) antigen on their red cells. You
react the patients serum with cells positive for certain antigens and see: K cells: 0, c
cells: 4+. What can be concluded - ANS You can rule out c antigen but cannot
confirm absence or presence of K antigen on patients red cells. Patient producing anti-c
and therefore does not have the c antigen. K did not react so patient either has K
antigen or has never been exposed to K and therefore does not make antibodies to K.
Urine protein chemistry dipstick detected no proteins by sulfosalicylic acid (SSA) test did
detect proteins. Why? - ANS Bence-jones proteins in urine
Several immature granulocytes in PBS. What stain should be used to figure this
persons problem out? - ANS LAP
Syndrome of inappropriate antidiuretic hormone secretions (SIADH) would result in
what in blood? - ANS Deficient in sodium
Fiber strands in urine resemble what under the microscope? - ANS Hyaline casts
Which of the following regulates myocyte contraction - ANS Cardiac troponins
HBA1C levels cannot always be used to monitor glucose levels in conditions such as:
- ANS Sickle cell disease; any disorder that causes RBCs to die prematurely (sickle
cell, g6pd etc) will result in underestimation of HBA1C levels
Strentrophomonas: - ANS Rapid oxidizers of maltose
Burr cells indicative of - ANS Uremia
Stain used for Cryptosporidium parvum - ANS Modified trichrome stain
Stomatocytes indicative of - ANS Liver disease
Acanthocytes: - ANS Inadequate drying
