MCB 250 Exam 3 Review questions with
100% complete solutions
Genetic Screen - correct answer ✔✔Organism is exposed to radiation or chemical
mutagen; mutagenesis. Then progeny is identified of that individual who displayed
mutant phenotype.
Microarray - correct answer ✔✔a flat surface with thousands of different DNA
sequences arranged in spots.
Single RNA - correct answer ✔✔Northern blot. qPCR.
Many RNAs - correct answer ✔✔Microarray. mRNA seq.
Requirements for a Cloning Vector - correct answer ✔✔1. origin of replication
recognized by host cell.
2. one or more selectible marker genes.
3. cloning sites; restriction enzyme sites.
What enzymes are used in DNA cloning? - correct answer ✔✔Restriction
enzymes: recognize and cut different base-pair sequences.
DNA ligase: form phosphodiester bonds between two compatible DNA ends.
DNA library - correct answer ✔✔Mixture of many different DNA sequences that
were cloned simultaneously into a large number of host cells.
, Genomic DNA library - correct answer ✔✔Contrains all of the DNA sequences
present within the chromosomes of the organism beign studied.
Sanger sequencing - correct answer ✔✔DNA sequencing method that determines
the order of nucleotides in a DNA segment.
ddATP, ddGTP, ddTTP, ddCTP - correct answer ✔✔A, G, T, C
cDNA - correct answer ✔✔collection of bacterial colonies, with each colony
containing seperate cDNA cloned into a plasmid.
Newly synthesized Sanger sequencing reading - correct answer ✔✔Read bottom
up.
Template strand - correct answer ✔✔Read top down, but theoretically it is the
antiparallel complementary sequence of the newly synthesized.
Medel's Obserbations: genotype vs. phenotype - correct answer ✔✔"Heritable
elements" (genes) specify traits. Genes come in different alleles. A dominant allele
hides a recessive allele and determines phenotype.
PCR - correct answer ✔✔1. denaturation (96 degrees C)
2. primer annealing (55 degrees C)
3. primer extension (72 degrees C)
Result after each cycle: # of DNA molecules doubled
100% complete solutions
Genetic Screen - correct answer ✔✔Organism is exposed to radiation or chemical
mutagen; mutagenesis. Then progeny is identified of that individual who displayed
mutant phenotype.
Microarray - correct answer ✔✔a flat surface with thousands of different DNA
sequences arranged in spots.
Single RNA - correct answer ✔✔Northern blot. qPCR.
Many RNAs - correct answer ✔✔Microarray. mRNA seq.
Requirements for a Cloning Vector - correct answer ✔✔1. origin of replication
recognized by host cell.
2. one or more selectible marker genes.
3. cloning sites; restriction enzyme sites.
What enzymes are used in DNA cloning? - correct answer ✔✔Restriction
enzymes: recognize and cut different base-pair sequences.
DNA ligase: form phosphodiester bonds between two compatible DNA ends.
DNA library - correct answer ✔✔Mixture of many different DNA sequences that
were cloned simultaneously into a large number of host cells.
, Genomic DNA library - correct answer ✔✔Contrains all of the DNA sequences
present within the chromosomes of the organism beign studied.
Sanger sequencing - correct answer ✔✔DNA sequencing method that determines
the order of nucleotides in a DNA segment.
ddATP, ddGTP, ddTTP, ddCTP - correct answer ✔✔A, G, T, C
cDNA - correct answer ✔✔collection of bacterial colonies, with each colony
containing seperate cDNA cloned into a plasmid.
Newly synthesized Sanger sequencing reading - correct answer ✔✔Read bottom
up.
Template strand - correct answer ✔✔Read top down, but theoretically it is the
antiparallel complementary sequence of the newly synthesized.
Medel's Obserbations: genotype vs. phenotype - correct answer ✔✔"Heritable
elements" (genes) specify traits. Genes come in different alleles. A dominant allele
hides a recessive allele and determines phenotype.
PCR - correct answer ✔✔1. denaturation (96 degrees C)
2. primer annealing (55 degrees C)
3. primer extension (72 degrees C)
Result after each cycle: # of DNA molecules doubled
