BIO353 Exam 2 (Mod 15-23) Questions
and Answers 100% Pass
True or False: It is important to be able to repeatedly fractionate the cell. - ✔✔True
True or False: Homogenization buffer can be identical to an intracellular buffer. -
✔✔True
After homogenization the first centrifugation pellets the ________. - ✔✔Nuclei
A sucrose gradient is used to purify ___ ___ away from other ___ ___ - ✔✔plasma
membrane, membrane components
An organic precipitation works because the solvent ________ disrupts the ______
___________ of the solute. - ✔✔ethanol, hydrogen bonding
The binding of the signal recognition particle to the nascent peptide _____ protein
synthesis. - ✔✔arrests
The function of the stop- transfer and start-transfer sequences is to make a _____
protein. - ✔✔transmembrane
True or False: BiP acts as a chaperone - ✔✔True
Katelyn Whitman, All Rights Reserved © 2025 1
, During vesicular transport, what is the correct sequences of events as a vesicle leaves
the organelle? - ✔✔Triskelion, clathrin, recycle, Rab, SNARE
The subunit of clathrin is _____ - ✔✔Triskelion
The preassembled carbohydrate tree is added on ___ residue. - ✔✔Asparagine
What is the role of microtubules in membrane transport? - ✔✔Transport vesicles from
ER to Golgi
Can COP I play a role in protein disulfide isomer position? - ✔✔Yes
Does constitutive exocytosis require more signal sequences than regulative exocytosis? -
✔✔No
Does the inside/outside rule apply only to regulative exocytosis? - ✔✔No
True or False: The carbohydrate tree added to proteins targeted to the primary
lysosome is added on an amino acid different from the amino acid where the
carbohydrate tree is added to a transmembrane protein. - ✔✔False
True or False: At the trans face of the Golgi, triskelion assembles into clathrin to cause
removal of the putative primary lysosome. - ✔✔True
Where is the number 6 carbon of mannose exposed?
-in the nucleus
Katelyn Whitman, All Rights Reserved © 2025 2
and Answers 100% Pass
True or False: It is important to be able to repeatedly fractionate the cell. - ✔✔True
True or False: Homogenization buffer can be identical to an intracellular buffer. -
✔✔True
After homogenization the first centrifugation pellets the ________. - ✔✔Nuclei
A sucrose gradient is used to purify ___ ___ away from other ___ ___ - ✔✔plasma
membrane, membrane components
An organic precipitation works because the solvent ________ disrupts the ______
___________ of the solute. - ✔✔ethanol, hydrogen bonding
The binding of the signal recognition particle to the nascent peptide _____ protein
synthesis. - ✔✔arrests
The function of the stop- transfer and start-transfer sequences is to make a _____
protein. - ✔✔transmembrane
True or False: BiP acts as a chaperone - ✔✔True
Katelyn Whitman, All Rights Reserved © 2025 1
, During vesicular transport, what is the correct sequences of events as a vesicle leaves
the organelle? - ✔✔Triskelion, clathrin, recycle, Rab, SNARE
The subunit of clathrin is _____ - ✔✔Triskelion
The preassembled carbohydrate tree is added on ___ residue. - ✔✔Asparagine
What is the role of microtubules in membrane transport? - ✔✔Transport vesicles from
ER to Golgi
Can COP I play a role in protein disulfide isomer position? - ✔✔Yes
Does constitutive exocytosis require more signal sequences than regulative exocytosis? -
✔✔No
Does the inside/outside rule apply only to regulative exocytosis? - ✔✔No
True or False: The carbohydrate tree added to proteins targeted to the primary
lysosome is added on an amino acid different from the amino acid where the
carbohydrate tree is added to a transmembrane protein. - ✔✔False
True or False: At the trans face of the Golgi, triskelion assembles into clathrin to cause
removal of the putative primary lysosome. - ✔✔True
Where is the number 6 carbon of mannose exposed?
-in the nucleus
Katelyn Whitman, All Rights Reserved © 2025 2
