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MBIO 3400 Lab Exam questions with complete solutions A+ graded

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MBIO 3400 Lab Exam questions with complete solutions A+ graded

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MBIO Lab 3400
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MBIO lab 3400
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MBIO lab 3400

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Uploaded on
January 20, 2025
Number of pages
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Written in
2024/2025
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MBIO 3400 Lab Exam questions with
complete solutions A+ graded

Microbiology - correct answer ✔✔The study of living organisms that are to small to see with the naked
eye



Why do we study microbiology? - correct answer ✔✔-Antibiotics/medical applications

-agriculture/food production

-ecology and environment

-models for cellular processes



Microbes are fantastic models for other forms of life because they have what qualities? - correct answer
✔✔-fast generation time (exponential growth)

-small size

-small genome (easy to manipulate)



Ubiquitous - correct answer ✔✔they occur everywhere



Microbes are the - correct answer ✔✔most successful life forms on earth



where are microbes? - correct answer ✔✔-soil

-air

-water

-food

-your body



there are more microbial cells than... - correct answer ✔✔human cells

,Microorganisms have adapted to... - correct answer ✔✔live in essentially every habitat



microbes can survive and grown under conditions of.... - correct answer ✔✔-extreme pressure (deep
sea)

-temperature (hot springs/ snow algae)

-extreme pH (acidic or basic)



Microorganisms include... - correct answer ✔✔eukaryotic (fungi, protozoans), prokaryotic (bacteria) and
archaea



Microbes are important to our environment because they can... - correct answer ✔✔-cause disease in
plants and animals

-be used to produce antibiotics and other pharmaceuticals

-can be used to produce a wide range of food products

-can be used in industry

-are important in nutrient cycling



we are always in close contact with... - correct answer ✔✔bacteria, fungi, protozoa, and other microbes



Activity 1: Growing microorganisms isolated from the air - correct answer ✔✔1. label the bottom of a
TSA plate and a PDA plate carefully with your name and activity number

2. remove the lids on both of your plates for 20 minutes

3. replace the lids on both of your plates. Put both plates into the 30 degree celsius incubator until the
next lab period

(incubate your TSA plate upside down and your PDA plate right side up)

4. in the next lab section, observe and draw the different colony types you see on your plate. Not all
colonies will be bacteria; fluffy cotton-like colonies are molds (fungi)



microbes are so small and light that.... - correct answer ✔✔air currents are constantly wafting them
around you, or they can be hitchhiking on dust products

, molds (fungi) - correct answer ✔✔fluffy, cotton-like colonies



TSA plate (Trypticase Soy Agar) - correct answer ✔✔incubate upside down



PDA plate (potato dextrose agar) - correct answer ✔✔incubate right side up



incubate TSA plates in the small incubator at... - correct answer ✔✔30 degrees celsius



In activity 1 when we opened our TSA and PDA plates... - correct answer ✔✔the sterile culture medium
will be exposed to the bacterial cells and fungal spores in the air. they will settle on the surface of a
medium and grow into visible colonies during the following incubation



dissecting microscopes are - correct answer ✔✔generally used for larger organisms



dissecting microscope - correct answer ✔✔magnification is generally 10-200 times more powerful than
the naked eye and does not use objectives



Compound light microscopes use - correct answer ✔✔lenses to magnify microscopic objects



Compound light microscope - correct answer ✔✔magnification can range between 100-1000X more
powerful than the naked eye and uses multiple objectives



Microorganisms are to small to be seen by - correct answer ✔✔the naked eye



relative size of viruses - correct answer ✔✔~ 0.1 micrometer



relative size of bacteria - correct answer ✔✔range from 1-5 micrometers in size



relative size of fungi - correct answer ✔✔range from 10 micrometers to macroscopic structures

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