Molecular Biology ASCP Practice UPDATED
ACTUAL Questions and CORRECT
Answers
This refers to a means by which large quantities of DNA can be amplified from a small amount
of DNA template:
A. PCR
B. DNA mass production
C. DNA synthesis
D. DNA birth - CORRECT ANSWER - PCR
With regards to PCR, which of the following is NOT accurate?
A. Denature strands at 94 degrees
B. Base pairing of primers at 60 degrees
C. Elongation of primers using Taq polymerase at 72 degrees
D. Filtration of DNA at 40 degrees - CORRECT ANSWER - Filtration of DNA at 40
degrees
Which of the following PCR techniques is the best one for detecting point mutation in DNA?
A. RT PCR
B. PCR
C. Allele-specific PCR
D. Anchored PCR - CORRECT ANSWER - Allele-Specific PCR
What happens after many cycles of PCR?
A. Exponential Amplification
B. Diminished Amplification
C. Product lysing
,D. DNA translation - CORRECT ANSWER - Exponential Amplification
Which of the following is NOT true about the Taq DNA polymerase?
A. It allows for the ability to use PCR to automate the process of amplification
B. It can be heat resistant
C. It is ubiquitous in nature except for the hot springs
D. It is most efficient at a certain optimal temperature. - CORRECT ANSWER -C
The PCR technique used to specifically amplify only one strand of the target DNA by using only
one unequal primer concentration is:
A. qPCR
B. Asymmetric PCR
C. Anchored PCR
D. Nested PCR - CORRECT ANSWER - Asymmetric PCR
Sarah wants to turn up the heat on her heat block to thaw her DNA samples at over 100 degrees
for an hour. What is the likely result of her PCR?
A. She will get much more amplified DNA product
B. She will not get a significant amplified DNA product
C. She will only get minimal synthesis
D. She will likely not get any synthesis - CORRECT ANSWER - She will likely not get
any synthesis
When setting up her thermocycler, Sarah wants to anneal her DNA strands at a temperature much
higher than 60 degrees. What is the likely result of her action?
A. The DNA strands will likely not anneal as desired.
B. The DNA strands will likely anneal more optimally.
C. The DNA strands will anneal, and then form a triple helix.
, D. The DNA strands will anneal but will not form a double helix. - CORRECT
ANSWER - The DNA strands will likely not anneal as desired.
The PCR technique widely used in bacterial genomic studies for screening of plasmid inserts is:
A. Anchored DNA
B. Colony PCR
C. qPCR
D. pPCR - CORRECT ANSWER - Colony PCR
When setting up her thermocycler, Sarah wants to base pair her DNA strands at a temperature
much lower than 60 degrees. What is the likely result of her action?
A. She will only get single-stranded DNA products
B. The primers will likely stick to other regions of the genome
C. The primers will stick to the 5' ends only
D. The primers will stick to the 3' ends only - CORRECT ANSWER - The primers will
likely stick to other regions of the genome.
Which of the following is true?
A. Elongation occurs at 90 degrees
B. Taq polymerase activity is near optimal at 72 degrees
C. Taq polymerase activity is near optimal at 50 degrees
D. Polymerase is not required for the elongation step of PCR - CORRECT ANSWER - Taq
polymerase activity is near optimal at 72 degrees.
This PCR variant uses degenerate primers to amplify unknown sequences of DNA, related to a
known DNA sequence is:
A. Degenerate PCR
B. Multiplex PCR
C. Hotstart PCR
ACTUAL Questions and CORRECT
Answers
This refers to a means by which large quantities of DNA can be amplified from a small amount
of DNA template:
A. PCR
B. DNA mass production
C. DNA synthesis
D. DNA birth - CORRECT ANSWER - PCR
With regards to PCR, which of the following is NOT accurate?
A. Denature strands at 94 degrees
B. Base pairing of primers at 60 degrees
C. Elongation of primers using Taq polymerase at 72 degrees
D. Filtration of DNA at 40 degrees - CORRECT ANSWER - Filtration of DNA at 40
degrees
Which of the following PCR techniques is the best one for detecting point mutation in DNA?
A. RT PCR
B. PCR
C. Allele-specific PCR
D. Anchored PCR - CORRECT ANSWER - Allele-Specific PCR
What happens after many cycles of PCR?
A. Exponential Amplification
B. Diminished Amplification
C. Product lysing
,D. DNA translation - CORRECT ANSWER - Exponential Amplification
Which of the following is NOT true about the Taq DNA polymerase?
A. It allows for the ability to use PCR to automate the process of amplification
B. It can be heat resistant
C. It is ubiquitous in nature except for the hot springs
D. It is most efficient at a certain optimal temperature. - CORRECT ANSWER -C
The PCR technique used to specifically amplify only one strand of the target DNA by using only
one unequal primer concentration is:
A. qPCR
B. Asymmetric PCR
C. Anchored PCR
D. Nested PCR - CORRECT ANSWER - Asymmetric PCR
Sarah wants to turn up the heat on her heat block to thaw her DNA samples at over 100 degrees
for an hour. What is the likely result of her PCR?
A. She will get much more amplified DNA product
B. She will not get a significant amplified DNA product
C. She will only get minimal synthesis
D. She will likely not get any synthesis - CORRECT ANSWER - She will likely not get
any synthesis
When setting up her thermocycler, Sarah wants to anneal her DNA strands at a temperature much
higher than 60 degrees. What is the likely result of her action?
A. The DNA strands will likely not anneal as desired.
B. The DNA strands will likely anneal more optimally.
C. The DNA strands will anneal, and then form a triple helix.
, D. The DNA strands will anneal but will not form a double helix. - CORRECT
ANSWER - The DNA strands will likely not anneal as desired.
The PCR technique widely used in bacterial genomic studies for screening of plasmid inserts is:
A. Anchored DNA
B. Colony PCR
C. qPCR
D. pPCR - CORRECT ANSWER - Colony PCR
When setting up her thermocycler, Sarah wants to base pair her DNA strands at a temperature
much lower than 60 degrees. What is the likely result of her action?
A. She will only get single-stranded DNA products
B. The primers will likely stick to other regions of the genome
C. The primers will stick to the 5' ends only
D. The primers will stick to the 3' ends only - CORRECT ANSWER - The primers will
likely stick to other regions of the genome.
Which of the following is true?
A. Elongation occurs at 90 degrees
B. Taq polymerase activity is near optimal at 72 degrees
C. Taq polymerase activity is near optimal at 50 degrees
D. Polymerase is not required for the elongation step of PCR - CORRECT ANSWER - Taq
polymerase activity is near optimal at 72 degrees.
This PCR variant uses degenerate primers to amplify unknown sequences of DNA, related to a
known DNA sequence is:
A. Degenerate PCR
B. Multiplex PCR
C. Hotstart PCR
