NHA Phlebotomy Certification Study Guide 2025 With Correct Answers

NHA Phlebotomy Certification Study
Guide 2025 With Correct Answers




Order Of Draw - correct answer 1) Sterile Blood Cultures
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2) Light Blue Top
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3) Red Top
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4) SST (Tiger Top) Serum Separator Tube
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5) PST (Light Green Top) Plasma Separator Tube
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6) Green Top
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7) Lavender Top
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8) Gray Top
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Sterile Blood Culture or (Yellow Top) - correct answer Additive: Media or SPS
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Tests: Looks for bacteria in the blood
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Light Blue Top - correct answer Additive: Sodium Citrate
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Type: Anticoagulant
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Tests: Coagulation Studies: PT, PTT, APTT
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Binds to calcium to prevent coagulation
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, Tube must be completely full 9:1 ratio
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Inverted 3-4 times k k




Red Top - correct answer Additive: None
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Type: Coagulant k k




Tests: Therapeutic Drug Levels k k k




Must stand for 30-60 minutes before centrifuging
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SST Serum Separator Tube (Tiger Top) - correct answer Additive: Silica (clotting activator), Gel (separator)
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Type: Coagulant k




Tests: Chemistry Panels, TSH, Hep B, HIV, Random Glucose
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Must stand for 30-60 minutes before centrifuging
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PST Plasma Separator Tube (Light Green Top) - correct answer Additives: Lithium Heparin, Gel
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Type: Anticoagulant k k




Tests: STAT Chemistries, STAT Electrolytes
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Green Top - correct answer Additive: Lithium, Sodium, Ammonium, Heparin
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Type: Anticoagulant k k




Tests: Ammonia (on the rocks), Chromosome analysis
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Lavender Top - correct answer Additive: EDTA k k k k k k




Type: Anticoagulant k




Tests: CBC, WBC, Sed Rate, ESR, Hemoglobin, Hematocrit
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Additive will bind to calcium and prevent blood from clotting
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Gray Top - correct answer Additive: Sodium Fluoride, Potassium Oxalate
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Type: Anticoagulant k




Tests: GTT, Fasting blood sugar (FBS), lactic acid (on ice), blood alcohol levels
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