Microbiology Lab Practical #1
Terms in this set (164)
Simple Stain
Negative Stain
Acid-Fast Stain
Capsule Stain
Endospore Stain
allow observation of living
Wet Mount and Hanging cells to determine motility
Drop
(prepared stain) allows for
observation of flagella
Flagella Stain
, differential/selective medium
Columbia CNA with 5% that allows for growth of Gram-
Sheep Blood Agar positive and inhibit growth of
Gram-negative organisms
Know the Microscopes
Parts!
supports the microscope
Base
Mechanical Stage
focuses light through the
specimen
Condenser
along with the base, used to
support the microscope when
Arm
carried
have magnification ranging
from 4x to 40x
Objective Lens
projects light upward through
the diaphragm and stage
Light Source/Illuminator
, regulates the amount of light
on the specimen
Diaphragm
has a magnification of 10x.; aka
eyepiece
Ocular Lens
rotates the objective lenses
Revolving Nosepiece
holds the slide in place
Stage Clips
where the specimen is placed
Slide
Specimen the object being studied
moves the stage up and down
for focusing
Coarse Adjustment Knob
moves the stage slightly to
sharpen the image
Fine Adjustment Knob
1. On the low-power objective, use the coarse
Know the proper objective only
procedure for focusing 2. When higher power objectives are used, the fine
the microscope: adjustment knob can be changed to bring the
object into focus.
Terms in this set (164)
Simple Stain
Negative Stain
Acid-Fast Stain
Capsule Stain
Endospore Stain
allow observation of living
Wet Mount and Hanging cells to determine motility
Drop
(prepared stain) allows for
observation of flagella
Flagella Stain
, differential/selective medium
Columbia CNA with 5% that allows for growth of Gram-
Sheep Blood Agar positive and inhibit growth of
Gram-negative organisms
Know the Microscopes
Parts!
supports the microscope
Base
Mechanical Stage
focuses light through the
specimen
Condenser
along with the base, used to
support the microscope when
Arm
carried
have magnification ranging
from 4x to 40x
Objective Lens
projects light upward through
the diaphragm and stage
Light Source/Illuminator
, regulates the amount of light
on the specimen
Diaphragm
has a magnification of 10x.; aka
eyepiece
Ocular Lens
rotates the objective lenses
Revolving Nosepiece
holds the slide in place
Stage Clips
where the specimen is placed
Slide
Specimen the object being studied
moves the stage up and down
for focusing
Coarse Adjustment Knob
moves the stage slightly to
sharpen the image
Fine Adjustment Knob
1. On the low-power objective, use the coarse
Know the proper objective only
procedure for focusing 2. When higher power objectives are used, the fine
the microscope: adjustment knob can be changed to bring the
object into focus.
