MT AAB Basic Knowledge
What agency approves new test methodologies, instruments and reagents before they
are available for purchase by consumers? - answer Food and Drug Administration
(FDA)
How often should biological safety hoods be inspected? - answer annually
What is the minimum number of standards required to form a standard curve? -
answer3
What is the maximum magnification using a brightfield microscope? - answer1000X
What is the most accurate pipette for reconstituting control material? - answer glass
volumetric pipettes
Which unit if measure refers to 10⁻³? - answer milli-
What is the calculated cholesterol/HDL ratio given the following results?
Cholesterol 225mg/dL
HDL 75mg/dL - answer225/75=
3.00
200 µL of patient serum is diluted with 800µL of saline. What is the final dilution? -
answer200+800=1000
200/1000=
1/5
1:5
What is the calculated LDL cholesterol given the following lipid profile results?
Triglycerides 190 mg/dL
Cholesterol 225 mg/dL
HDL 75 mg/dL - answer112
How is relative centrifugal force calculated? - answer1.18 X 10⁻⁵ x r x rpm²
A chain of custody is required for which proceedings? - answerlegal blood alcohol, pre-
employment drug screen and paternity testing
, not required for fetal fibronectin
What federal legislation regulates most clinical laboratories? - answerClinical Laboratory
Improvement Amendments (CLIA)
What is the level of complexity of most testing performed in clinical laboratories? -
answermoderately complex
How often does CLIA require clinical laboratories to have inspections? - answerevery 2
years
Which laboratories are required to participate in proficiency testing? - answerreference
laboratories, laboratories performing moderately complex testing, and laboratories
performing highly complex testing
not required for laboratories performing waived testing only
Which proficiency testing (PT) results do not meet CLIA performance criteria? -
answerfailed the same analyte in two consecutive PT events
What is an example of alternative proficiency testing? - answersplit samples sent to
another facility using the same test method
What are pre-analytical errors? - answerordering a lab test under the wrong patient
number, misidentification of a patient, mislabeling an add-on test
not pre-analytical-transcription error entering a manual result
What is the most common analytical error? - answernot checking specimen integrity
What is the most common post-analytical error? - answerclerical errors
How often must a procedure be reviewed? - answerupon implementation and
substantial revisions
How often must thermometers be checked against the NIST thermometer? -
answerannually
In which manual are the directions for running quality control for specific assay kept? -
answeroperating procedure manual
Following equipment maintenance, when is quality control suggested? - answerafter
any preventative maintenance
What is the most common reason for a control value to be outside of 3SD? -
answerrandom error
What agency approves new test methodologies, instruments and reagents before they
are available for purchase by consumers? - answer Food and Drug Administration
(FDA)
How often should biological safety hoods be inspected? - answer annually
What is the minimum number of standards required to form a standard curve? -
answer3
What is the maximum magnification using a brightfield microscope? - answer1000X
What is the most accurate pipette for reconstituting control material? - answer glass
volumetric pipettes
Which unit if measure refers to 10⁻³? - answer milli-
What is the calculated cholesterol/HDL ratio given the following results?
Cholesterol 225mg/dL
HDL 75mg/dL - answer225/75=
3.00
200 µL of patient serum is diluted with 800µL of saline. What is the final dilution? -
answer200+800=1000
200/1000=
1/5
1:5
What is the calculated LDL cholesterol given the following lipid profile results?
Triglycerides 190 mg/dL
Cholesterol 225 mg/dL
HDL 75 mg/dL - answer112
How is relative centrifugal force calculated? - answer1.18 X 10⁻⁵ x r x rpm²
A chain of custody is required for which proceedings? - answerlegal blood alcohol, pre-
employment drug screen and paternity testing
, not required for fetal fibronectin
What federal legislation regulates most clinical laboratories? - answerClinical Laboratory
Improvement Amendments (CLIA)
What is the level of complexity of most testing performed in clinical laboratories? -
answermoderately complex
How often does CLIA require clinical laboratories to have inspections? - answerevery 2
years
Which laboratories are required to participate in proficiency testing? - answerreference
laboratories, laboratories performing moderately complex testing, and laboratories
performing highly complex testing
not required for laboratories performing waived testing only
Which proficiency testing (PT) results do not meet CLIA performance criteria? -
answerfailed the same analyte in two consecutive PT events
What is an example of alternative proficiency testing? - answersplit samples sent to
another facility using the same test method
What are pre-analytical errors? - answerordering a lab test under the wrong patient
number, misidentification of a patient, mislabeling an add-on test
not pre-analytical-transcription error entering a manual result
What is the most common analytical error? - answernot checking specimen integrity
What is the most common post-analytical error? - answerclerical errors
How often must a procedure be reviewed? - answerupon implementation and
substantial revisions
How often must thermometers be checked against the NIST thermometer? -
answerannually
In which manual are the directions for running quality control for specific assay kept? -
answeroperating procedure manual
Following equipment maintenance, when is quality control suggested? - answerafter
any preventative maintenance
What is the most common reason for a control value to be outside of 3SD? -
answerrandom error
