MT (AAB) Basic Knowledge Test
Parts of a centrifuge - answer Rotor, Carriers, Shield
Three variables of centrifugal force - answer Mass, Speed, Radius
The speed of a centrifuge is related to relative centrifugal force (RCF) by this equation: -
answer1.118 x 10^-5 x r x (rpm)^2
Blood samples should be centrifuged at - answer1,000-1,200 xg OR 3,500 rpm for 10-
15 min
Four types of centrifuges - answer Horizontal Head, Fixed angle, Micro, Ultra
How does one check the speed of a centrifuge - answer using a tachometer or strobe
light
How often should the speed on a centrifuge be checked/calibration completed - answer
every 6 months
Classes of glassware - answer Class A and Class B-Class be has 2x the tolerance limit
of Class A
What glassware class are pipettes - answerClass A
What are the 9 different kinds of pipettes - answerAutomated, Semi-automated, Manual,
Transfer/Volumetric, Measuring/Graduated, To Contain (TC), To Deliver (TD), Blow out,
Self Draining
Automated Pipette - answerElectronic-used to prepare multiple samples for analysis
Semi Automated Pipette - answerHandheld-use disposable tips and either air
displacement of positive displacement
Manual Pipettes - answerHandheld-glass or plastic that require a bulb to apply suction
Transfer/Volumetric Pipette - answerGlass Pipette that has a bulb in the pipette with a
tapered delivery tip Accuracy 1:1,000
Measuring/Graduated Pipette - answerGlass or plastic pipette uniform in diameter with
tapered delivery tip
Parts of a centrifuge - answer Rotor, Carriers, Shield
Three variables of centrifugal force - answer Mass, Speed, Radius
The speed of a centrifuge is related to relative centrifugal force (RCF) by this equation: -
answer1.118 x 10^-5 x r x (rpm)^2
Blood samples should be centrifuged at - answer1,000-1,200 xg OR 3,500 rpm for 10-
15 min
Four types of centrifuges - answer Horizontal Head, Fixed angle, Micro, Ultra
How does one check the speed of a centrifuge - answer using a tachometer or strobe
light
How often should the speed on a centrifuge be checked/calibration completed - answer
every 6 months
Classes of glassware - answer Class A and Class B-Class be has 2x the tolerance limit
of Class A
What glassware class are pipettes - answerClass A
What are the 9 different kinds of pipettes - answerAutomated, Semi-automated, Manual,
Transfer/Volumetric, Measuring/Graduated, To Contain (TC), To Deliver (TD), Blow out,
Self Draining
Automated Pipette - answerElectronic-used to prepare multiple samples for analysis
Semi Automated Pipette - answerHandheld-use disposable tips and either air
displacement of positive displacement
Manual Pipettes - answerHandheld-glass or plastic that require a bulb to apply suction
Transfer/Volumetric Pipette - answerGlass Pipette that has a bulb in the pipette with a
tapered delivery tip Accuracy 1:1,000
Measuring/Graduated Pipette - answerGlass or plastic pipette uniform in diameter with
tapered delivery tip
