Lab 5: Sensory Transduction (SIMULATION)
1. What is the effect of the TRPV1 channel opening ? positive ions flow
inwards
2. What is an important characteristic of the buffer used one that blocks
for studying sodium in patch clamp experiments? potassium and cal-
cium channels
3. If this cell is a neuron , using the voltage clamp to A current that in-
change the cell membrane potential from - 70.0mV to dicates a large
- 5.0mV should cause: amount of sodium
briefly entering the
cell
4. Looking at the graph, these data are consistent with Capsaicin opens
which statement below? the TRPV1 chan-
nels with no effect
on sodium chan-
nels
5. The step from -60 to -5 on the top graph represents Lidocaine inhibits
the clamped voltage, and the bottom graph represents the voltage-gated
the current through the sodium channel.You can com- sodium channels
pare to the control and capsaicin effect by clicking on
those buttons on the computer screen. What do these
graphs tell us about the action of Lidocaine?
6. What does this output tell us about the action of the Rebine seems to
drug Rebine? have no impact on
the cell
7. Together, all the experiments so far are consistent Rebine requires
with: open TRPV1
channels in or-
der to inhibit volt-
age-gated chan-
nels
8. What would be the effect on the membrane voltage Membrane voltage
of tripling the clamped current while applying Rebine will increase with-
and capsaicin together to the nociceptor?
1/2
1. What is the effect of the TRPV1 channel opening ? positive ions flow
inwards
2. What is an important characteristic of the buffer used one that blocks
for studying sodium in patch clamp experiments? potassium and cal-
cium channels
3. If this cell is a neuron , using the voltage clamp to A current that in-
change the cell membrane potential from - 70.0mV to dicates a large
- 5.0mV should cause: amount of sodium
briefly entering the
cell
4. Looking at the graph, these data are consistent with Capsaicin opens
which statement below? the TRPV1 chan-
nels with no effect
on sodium chan-
nels
5. The step from -60 to -5 on the top graph represents Lidocaine inhibits
the clamped voltage, and the bottom graph represents the voltage-gated
the current through the sodium channel.You can com- sodium channels
pare to the control and capsaicin effect by clicking on
those buttons on the computer screen. What do these
graphs tell us about the action of Lidocaine?
6. What does this output tell us about the action of the Rebine seems to
drug Rebine? have no impact on
the cell
7. Together, all the experiments so far are consistent Rebine requires
with: open TRPV1
channels in or-
der to inhibit volt-
age-gated chan-
nels
8. What would be the effect on the membrane voltage Membrane voltage
of tripling the clamped current while applying Rebine will increase with-
and capsaicin together to the nociceptor?
1/2
