Microbiology Lab Exam 1 Questions
with Complete Solutions
when viewing a smear with the oil immersion lens the final magnification is Correct Ans-
1000x;10x ocular x 100X objective
what is the purpose of heat fixing Correct Ans-kill the bacteria, adhere to glass anddenature
the proteins of bacteria
name oneof the stains or regents used in the gram stain procedure Correct Ans-gram crystal
violet, gram iodine, gram sfaranin, 95% ethanol
why are aseptic techniques used in the lab? Correct Ans-to ensure that we do not contaminate
what we are working with as as to ensue that we do not acciently inoculate ouselves. example of
aseptic techniques are flaming the loop/needle sterilizing it, breifly flaming the test tube before
and after obtaining the sample.
the gram reaction is due to differences in the structure of wat part of the microbe Correct Ans-
its cell wall, specifically peptidoglycan, gram positive bacteria have thicker peptidoglycan layer
compared to GN bacteria.
Name 2 inoculating tools you will be using in this lab Correct Ans-loop, needle
what color is a gram positive microbe Correct Ans-purple
, waht is the function of the ethanol in the gram stain procedure Correct Ans-decolorize
name one of the strains in the acid fast stain Correct Ans-cold kinyoun, carbol fuschsin,
methylen blue
name one of the stains in the endospore stain Correct Ans-malachite green, safranin
what is the purpose of the acid-fast stain Correct Ans-the purpose of the acid-fast stain is to
differentiate between the myobactriace family of bacteria from all other bacteria
what color does na acid-fast microbe stain with acd-fast stain Correct Ans-red
what is the function of agarin the media Correct Ans-agar allows media to remain solid at
room temperature
what is the difference between broth and agar based media Correct Ans-broth is liquid media
at room temperature, agar media is solid at room temp.
what is the funciton of media Correct Ans-media is used to provide nutrients to a microbe as
well as a growth medium
what is the name of the plate inoculation technique Correct Ans-quadrant steak plate
with Complete Solutions
when viewing a smear with the oil immersion lens the final magnification is Correct Ans-
1000x;10x ocular x 100X objective
what is the purpose of heat fixing Correct Ans-kill the bacteria, adhere to glass anddenature
the proteins of bacteria
name oneof the stains or regents used in the gram stain procedure Correct Ans-gram crystal
violet, gram iodine, gram sfaranin, 95% ethanol
why are aseptic techniques used in the lab? Correct Ans-to ensure that we do not contaminate
what we are working with as as to ensue that we do not acciently inoculate ouselves. example of
aseptic techniques are flaming the loop/needle sterilizing it, breifly flaming the test tube before
and after obtaining the sample.
the gram reaction is due to differences in the structure of wat part of the microbe Correct Ans-
its cell wall, specifically peptidoglycan, gram positive bacteria have thicker peptidoglycan layer
compared to GN bacteria.
Name 2 inoculating tools you will be using in this lab Correct Ans-loop, needle
what color is a gram positive microbe Correct Ans-purple
, waht is the function of the ethanol in the gram stain procedure Correct Ans-decolorize
name one of the strains in the acid fast stain Correct Ans-cold kinyoun, carbol fuschsin,
methylen blue
name one of the stains in the endospore stain Correct Ans-malachite green, safranin
what is the purpose of the acid-fast stain Correct Ans-the purpose of the acid-fast stain is to
differentiate between the myobactriace family of bacteria from all other bacteria
what color does na acid-fast microbe stain with acd-fast stain Correct Ans-red
what is the function of agarin the media Correct Ans-agar allows media to remain solid at
room temperature
what is the difference between broth and agar based media Correct Ans-broth is liquid media
at room temperature, agar media is solid at room temp.
what is the funciton of media Correct Ans-media is used to provide nutrients to a microbe as
well as a growth medium
what is the name of the plate inoculation technique Correct Ans-quadrant steak plate
