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Chapter 3 - Microbiology Lab Exam
Questions And Answers 100% Pass.
Name the parts of the Compound Light Microscope: Eye piece, Objective, Stage, Light Control,
Base, Focus - Answer✔
Dark-field Microscope - Answer✔used when the microbes are not visible with the use of the
light microscope;
when microbes cannot be stained;
when microbes would be distorted by staining
Phase-Contrast Microscope - Answer✔allows detailed visualization of the internal structures in
living microbes
Fluorescence Microscope - Answer✔Immunofluorescence;
detecting bacteria and other pathogens within cells and tissues;
Dx of rabies and Syphilis
Electron Microscope - Answer✔ability to visualize viruses, the internal structure of cells, and
other objects smaller than 0.2 um;
uses a beam of electrons
Transmission electron microscope/TEM - Answer✔utilizes a focused beam of electrons that are
emitted from an electron gun and pass thru a very thin section of the specimen
Scanning electron microscope/SEM - Answer✔section of specimen does NOT have to be thinly
sliced like with the TEM;
3D views are obtained
Which staining method is sufficient for determining the shape of bacteria & viewing basic
structures? - Answer✔simple stain
What is mordant? - Answer✔chemical additive added to the stain to increase the function of the
stain
What are the 2 most frequently used differential stains? - Answer✔Gram stain;
Acid-fast stain
Chapter 3 - Microbiology Lab Exam
Questions And Answers 100% Pass.
Name the parts of the Compound Light Microscope: Eye piece, Objective, Stage, Light Control,
Base, Focus - Answer✔
Dark-field Microscope - Answer✔used when the microbes are not visible with the use of the
light microscope;
when microbes cannot be stained;
when microbes would be distorted by staining
Phase-Contrast Microscope - Answer✔allows detailed visualization of the internal structures in
living microbes
Fluorescence Microscope - Answer✔Immunofluorescence;
detecting bacteria and other pathogens within cells and tissues;
Dx of rabies and Syphilis
Electron Microscope - Answer✔ability to visualize viruses, the internal structure of cells, and
other objects smaller than 0.2 um;
uses a beam of electrons
Transmission electron microscope/TEM - Answer✔utilizes a focused beam of electrons that are
emitted from an electron gun and pass thru a very thin section of the specimen
Scanning electron microscope/SEM - Answer✔section of specimen does NOT have to be thinly
sliced like with the TEM;
3D views are obtained
Which staining method is sufficient for determining the shape of bacteria & viewing basic
structures? - Answer✔simple stain
What is mordant? - Answer✔chemical additive added to the stain to increase the function of the
stain
What are the 2 most frequently used differential stains? - Answer✔Gram stain;
Acid-fast stain
