© EVERLY 2025 ALL RIGHTS RESERVED.
Microbiology Lab Practical 1 Study Guide
Questions And Answers 100% Pass.
Illuminator - Answer✔Filter for shorter λ
Condenser microscope - Answer✔Focuses light into specimen
Diaphragm (iris) - Answer✔Controls amount of light to specimen
Objective lenses at nosepiece - Answer✔Magnifies image 10, 40, or 100 times
Ocular lens - Answer✔magnifies image 10X
Course adjustment - Answer✔focuses specimen in a broad way
Fine adjustment - Answer✔fine focuses specimen
Stage - Answer✔a platform used to hold the slide containing the specimen
Arm - Answer✔serves as a handle for carrying microscope
Mechanical stage adjuster - Answer✔moves stage forward and back
Stage clip - Answer✔Holds microscope slide in position
Magnification - Answer✔increase in apparent size
Resolution - Answer✔clarity
Oil has the __________ refractive index as glass - Answer✔same
This is the part of the microscope that should not be
touched under high power. - Answer✔the course adjustment knob
How does the light intensity change when a student goes from a low power objective to a high
power objective? - Answer✔The field of vision narrowed
Which lens do you use oil with? - Answer✔100X lens
How does immersion oil work to enhance resolution? - Answer✔Same refractive index as glass;
prevents loss of light to air
, © EVERLY 2025 ALL RIGHTS RESERVED.
What is the maximum resolution of your microscope (in micrometers) or how far apart do two
organisms have to be from one another in order to see them as 2 different organisms under the
scope? - Answer✔.2 um with 100X
What is the total magnification of your scope when you use each lens? - Answer✔ocular lens
magnification times the magnification of the objective lens 10X x 40X, 100X, 10X
What do you have to do to your microscope before you put it away? - Answer✔lower the stage
If your cells look washed out under the scope what can you do to fix the lighting -
Answer✔Adjust the lighting by using diaphragm or condenser
Binocular versus monocular - Answer✔Binocular has two eyepieces. Monocular has one
eyepiece.
Inorder to increase the resolution under the 100X oil objective, you must open this part of the
microscope. - Answer✔diaphragm
path of light through a microscope and tell what happens after the light passes through each part.
- Answer✔
Eukaryote - Answer✔have a nucleus, membrane bound organelles and are larger than
prokaryotes
Prokaryote - Answer✔no nucleus, no membrane bound organelles and are small
If you went from the 40X objective to the 100X objective and the cell you were looking at
disappeared, give several explanations explaining what could have happened and how you could
fix the problem. - Answer✔Field of vision narrowed—center specimen
Not enough light—open diaphragm
Slightly out of focus—use fine focus
The lens is not clicked all the way in place
Cellular morphology - Answer✔cell shape
3 types of cell shapes CBS - Answer✔coccus (spheres), bacillus (rods), and spirillum (spiral)
Field of vision - Answer✔area you can see, smaller at higher powers; must CENTER object or
you will lose it at higher power
Refractive index - Answer✔the ratio of light in a vacuum to its velocity in a specified medium
Parfocal - Answer✔when you can switch from one power to the next and you would only need to
slightly fine focus with fine-adjustment knob to get specimen into view.
2 ways in which mold colonies differ from bacterial colonies under the dissecting scope -
Answer✔Mold colonies can't really be counted because they grow irregularly and in odd shapes.
Bacteria colonies can be counted. Mold colonies are big and fuzzy looking while bacteria
colonies are small, colorful, smooth or rough looking. Mold colonies usually look grey.
Microbiology Lab Practical 1 Study Guide
Questions And Answers 100% Pass.
Illuminator - Answer✔Filter for shorter λ
Condenser microscope - Answer✔Focuses light into specimen
Diaphragm (iris) - Answer✔Controls amount of light to specimen
Objective lenses at nosepiece - Answer✔Magnifies image 10, 40, or 100 times
Ocular lens - Answer✔magnifies image 10X
Course adjustment - Answer✔focuses specimen in a broad way
Fine adjustment - Answer✔fine focuses specimen
Stage - Answer✔a platform used to hold the slide containing the specimen
Arm - Answer✔serves as a handle for carrying microscope
Mechanical stage adjuster - Answer✔moves stage forward and back
Stage clip - Answer✔Holds microscope slide in position
Magnification - Answer✔increase in apparent size
Resolution - Answer✔clarity
Oil has the __________ refractive index as glass - Answer✔same
This is the part of the microscope that should not be
touched under high power. - Answer✔the course adjustment knob
How does the light intensity change when a student goes from a low power objective to a high
power objective? - Answer✔The field of vision narrowed
Which lens do you use oil with? - Answer✔100X lens
How does immersion oil work to enhance resolution? - Answer✔Same refractive index as glass;
prevents loss of light to air
, © EVERLY 2025 ALL RIGHTS RESERVED.
What is the maximum resolution of your microscope (in micrometers) or how far apart do two
organisms have to be from one another in order to see them as 2 different organisms under the
scope? - Answer✔.2 um with 100X
What is the total magnification of your scope when you use each lens? - Answer✔ocular lens
magnification times the magnification of the objective lens 10X x 40X, 100X, 10X
What do you have to do to your microscope before you put it away? - Answer✔lower the stage
If your cells look washed out under the scope what can you do to fix the lighting -
Answer✔Adjust the lighting by using diaphragm or condenser
Binocular versus monocular - Answer✔Binocular has two eyepieces. Monocular has one
eyepiece.
Inorder to increase the resolution under the 100X oil objective, you must open this part of the
microscope. - Answer✔diaphragm
path of light through a microscope and tell what happens after the light passes through each part.
- Answer✔
Eukaryote - Answer✔have a nucleus, membrane bound organelles and are larger than
prokaryotes
Prokaryote - Answer✔no nucleus, no membrane bound organelles and are small
If you went from the 40X objective to the 100X objective and the cell you were looking at
disappeared, give several explanations explaining what could have happened and how you could
fix the problem. - Answer✔Field of vision narrowed—center specimen
Not enough light—open diaphragm
Slightly out of focus—use fine focus
The lens is not clicked all the way in place
Cellular morphology - Answer✔cell shape
3 types of cell shapes CBS - Answer✔coccus (spheres), bacillus (rods), and spirillum (spiral)
Field of vision - Answer✔area you can see, smaller at higher powers; must CENTER object or
you will lose it at higher power
Refractive index - Answer✔the ratio of light in a vacuum to its velocity in a specified medium
Parfocal - Answer✔when you can switch from one power to the next and you would only need to
slightly fine focus with fine-adjustment knob to get specimen into view.
2 ways in which mold colonies differ from bacterial colonies under the dissecting scope -
Answer✔Mold colonies can't really be counted because they grow irregularly and in odd shapes.
Bacteria colonies can be counted. Mold colonies are big and fuzzy looking while bacteria
colonies are small, colorful, smooth or rough looking. Mold colonies usually look grey.
