High-Yield C/P MCAT topics questions and answers with solutions 2025

a hyperbolic dependence on [S] - ANSWER what shape does the graph of V0 versus substrate concentration [S] have in traditional Michaelis-Menten kinetics? -1/Km - ANSWER what is the x-intercept in a linewearver-burke plot? 1/Vmax - ANSWER what is the y-intercept in a linewearver-burke plot? Cytosine - ANSWER Guanine - ANSWER Adenine - ANSWER Thymine - ANSWER adenine and guanine - ANSWER What are the purines? cytosine and thymine - ANSWER What are the pyrimidines? SDS-PAGE - ANSWER denatures proteins and gives them a negative uniform charge. Thus, they are purely separated on size alone, with the smaller polypeptide chain migrating further than the larger polypeptide chains. Has a reducing and non-reducing component. Reducing SDS-PAGE - ANSWER reduces disulfide bonds and breaks quaternary structure Non-reducing SDS-PAGE - ANSWER does not cleave disulfide bonds, destroys quaternary and tertiary structure Native PAGE - ANSWER disulfide bonds intact, quaternary structure intact, separates proteins by mass, charge and polarity. More compact structures migrate further hydrophilic - ANSWER are peptides hydrophilic or hydrophobic? hydrophobic - ANSWER are steroids h