Define Standard Precautions - Answers a comprehensive set of safety guidelines for healthcare workers,
which include components of both Universal Precautions and BSI Guidelines. Thus "Standard
Precautions" is the current terminology and the fundamental premise employed by healthcare personal
when rendering care to every patient. The intent of standard Precautions is to protect patients and
healthcare workers.
PPE - Answers Personal Protective Equipment
Ex: gloves, goggles, mask, face shield, eye shield
Most important way of preventing both CIA and HAI - Answers Hand Hygiene techniques
Define: Sterilization - Answers refers to the killing of inactivation of all living organisms and viruses
Define: disinfection - Answers are chemicals used on inanimate objects or surfaces, such as counter
tops, floors, instruments, or lab ware, to kill or inactivate microorganisms
Define: antisepsis - Answers Are chemicals used on skin or tissue to inhibit growth of microbes.
6 types of lab ware - Answers 1.Bottles
2. Beakers
3. flasks
4. Test tubes
5. Graduated cylinder
6. Pipets
Centrifuges: name 3 kinds used in the laboratory - Answers clinical centrifuge
serological centrifuge
microcentrifuge
What is the basic metric unit of distance - Answers meter
What is the basic metric unit of length - Answers meter
What is the basic metric unit of volume - Answers Liter
What is the basic metric unit of weight - Answers grams
Convert inches to cm - Answers 1 in. = 2.54cm
, 3 in = 7.62cm
Convert mm to inches - Answers 1 mm. = 0.1 cm
45 mm. = 4.5 cm.
Convert g to mg - Answers 1 g. = 0.001 kg
80 g. = 0.08
The marking TD on a pipet - Answers A to deliver pipet is marked with the letters TD near the top of the
pipet. This means that the pipet is calibrated to deliver the volume marked on the pipet in a specified
time and at a specified temperature when the pipet is emptied by gravity drainage. When used
correctly, the TD pipet delivers the specified volume of fluid, leaving a small drop of fluid in the pipet tip.
A TD pipet with a frosted band etched around the top of the pipet is called a blowout pipet.
What is the difference in serological and volumetric? - Answers Serological- have a total capacity
marking near the suction end and are usually labeled TD. Are graduated to the tip, with markings
indicating uniform increments. These pipets can be used to transfer total capacity or partial volume.
These pipets usually marked TD and have frosted band. Volumetric- have a wide opening on the
suctioning end, an oval bulb in the center, and a tapered tip on the dispensing end. Are usually labeled
TD and are used when critical measurements are required, such as when preparing standards. Each
volumetric pipet is marked and calibrated to deliver only one volume. Volumetric pipets are used by
attaching a suctioning aid or pipet filer to the top of the pipet.
Prepare 100ml of 0.1 M HCl using 1 M HCl. C1 x V1 = C2 x V2 - Answers (C1)(V1)=(C2)(V2) (100ml)
(0.1M)= (C2)(1M). Divide (100ml)(0.1M) by 1M HCl V2= 10ml of 1M HCl is added to 90ml of H2O to make
100mL of 0.1 M Hal solution.
Serial dilution - - Answers a sample is dilated a number of times by the same dilution factor
Name a preanalytical factor - Answers patient identification procedure
Name a analytical factor - Answers use of standards and procedural controls
Name a postanalytical factor - Answers entering an incorrect patient identification number in the
computer.
Accuracy - Answers refers to the closeness of a measured value to the actual value, or true, value
Ex: Suppose that the true value for an individual's serum protein is 75 g/L. Then suppose the individuals
serum was analyzed three times by another method yielding results of 71g/L, 79g/L, and 75g/L, and all
three times by another method yielding results of 66, 69, and 68g/L. Imagine that the true value (75g/L)
is the center of a bull's eye target. Accurate values placed on the target would be clustered closely
around the true value. The set of analyses of 71, 79, 75g/L would be more accurate than the other set
because they are closer to the true value.