Strasinger AUBF - Fecal Analysis
Ultimate Reviewer Exam Questions and
Answers | Graded A+
Microscopic examination - ✔✔Performed to detect the presence of
leukocytes associated with microbial diarrhea and undigested muscle fibers
and fats associated with steatorrhea
Fecal Leukocytes - ✔✔Presence or absence of fecal neutrophils can
provide the physician with diagnostic information before receiving the
culture report
methylene blue - ✔✔stain used for wet preparation of fecal leukocytes
Wright's stain, Gram stain - ✔✔stain used for Dried smears of fecal
leukocytes
Methylene blue staining - ✔✔Staining: faster but difficult to interpret
Wright's or Gram stain - ✔✔stain that provides permanent slides for
evaluation
1
©JOSHCLAY 2024/2025. YEAR PUBLISHED 2024.
,Gram stain - ✔✔stain: additional advantage is the observation of gram-
positive and gram-negative bacteria which could aid in the initial treatment
Invasive condition - ✔✔presence of 3 neutrophils/hPf
Lactofferin Latex Agglutination test - ✔✔available for detecting fecal
leukocytes and remains sensitive in refrigerated and frozen specimens
Lactofferin - ✔✔component of granulocyte secondary granules
lactoferrin - ✔✔presence indicates invasive bacterial pathogen
Loffler methylene blue - ✔✔reagent used in Methylene Blue Stain for Fecal
Leukocytes
Muscle Fibers - ✔✔Microscopic examination of the feces for undigested
striated muscle fibers can be helpful in diagnosing and monitoring patients
with pancreatic insufficiency
biliary obstruction, gastrocolic fistulas - ✔✔Increased amount of striated
fibers may be seen in ___
10% alcoholic eosin - ✔✔Reagent used in test for muscle fibers, use to
emulsify a small amount of stool
10% alcoholic eosin - ✔✔enhances muscle fiber striations
5 mins - ✔✔Entire slide is examined for exactly ___ mins
2
©JOSHCLAY 2024/2025. YEAR PUBLISHED 2024.
, red-stained fibers - ✔✔Number of ____ with well-preserved striations is
counted
Undigested fibers - ✔✔visible striations running both vertically and
horizontally
Partially digested fibers - ✔✔exhibit striations in only one direction
Digested fibers - ✔✔no visible striations
undigested - ✔✔Only ____ fibers are counted
increased - ✔✔presence of more than 10 is reported as ____
fat malabsorption test - ✔✔other name for qualitative fecal fats tests
neutral fats (triglycerides), fatty acid salts (soaps), fatty acids, cholesterol -
✔✔Lipids that are included in the microscopic examination of feces
Sudan III, Sudan IV, Oil Red O - ✔✔Dyes used for lipids
Sudan III - ✔✔most routinely used dye
Neutral fats - ✔✔readily stained by Sudan III
large orange-red droplets - ✔✔Neutral fats appear as _____
Neutral fats - ✔✔often located near the edge of the cover slip
Steatorrhea - ✔✔> 60 droplets/hpf (neutral fats)
Split fat - ✔✔both number and size of fat droplets must be considered
3
©JOSHCLAY 2024/2025. YEAR PUBLISHED 2024.
Ultimate Reviewer Exam Questions and
Answers | Graded A+
Microscopic examination - ✔✔Performed to detect the presence of
leukocytes associated with microbial diarrhea and undigested muscle fibers
and fats associated with steatorrhea
Fecal Leukocytes - ✔✔Presence or absence of fecal neutrophils can
provide the physician with diagnostic information before receiving the
culture report
methylene blue - ✔✔stain used for wet preparation of fecal leukocytes
Wright's stain, Gram stain - ✔✔stain used for Dried smears of fecal
leukocytes
Methylene blue staining - ✔✔Staining: faster but difficult to interpret
Wright's or Gram stain - ✔✔stain that provides permanent slides for
evaluation
1
©JOSHCLAY 2024/2025. YEAR PUBLISHED 2024.
,Gram stain - ✔✔stain: additional advantage is the observation of gram-
positive and gram-negative bacteria which could aid in the initial treatment
Invasive condition - ✔✔presence of 3 neutrophils/hPf
Lactofferin Latex Agglutination test - ✔✔available for detecting fecal
leukocytes and remains sensitive in refrigerated and frozen specimens
Lactofferin - ✔✔component of granulocyte secondary granules
lactoferrin - ✔✔presence indicates invasive bacterial pathogen
Loffler methylene blue - ✔✔reagent used in Methylene Blue Stain for Fecal
Leukocytes
Muscle Fibers - ✔✔Microscopic examination of the feces for undigested
striated muscle fibers can be helpful in diagnosing and monitoring patients
with pancreatic insufficiency
biliary obstruction, gastrocolic fistulas - ✔✔Increased amount of striated
fibers may be seen in ___
10% alcoholic eosin - ✔✔Reagent used in test for muscle fibers, use to
emulsify a small amount of stool
10% alcoholic eosin - ✔✔enhances muscle fiber striations
5 mins - ✔✔Entire slide is examined for exactly ___ mins
2
©JOSHCLAY 2024/2025. YEAR PUBLISHED 2024.
, red-stained fibers - ✔✔Number of ____ with well-preserved striations is
counted
Undigested fibers - ✔✔visible striations running both vertically and
horizontally
Partially digested fibers - ✔✔exhibit striations in only one direction
Digested fibers - ✔✔no visible striations
undigested - ✔✔Only ____ fibers are counted
increased - ✔✔presence of more than 10 is reported as ____
fat malabsorption test - ✔✔other name for qualitative fecal fats tests
neutral fats (triglycerides), fatty acid salts (soaps), fatty acids, cholesterol -
✔✔Lipids that are included in the microscopic examination of feces
Sudan III, Sudan IV, Oil Red O - ✔✔Dyes used for lipids
Sudan III - ✔✔most routinely used dye
Neutral fats - ✔✔readily stained by Sudan III
large orange-red droplets - ✔✔Neutral fats appear as _____
Neutral fats - ✔✔often located near the edge of the cover slip
Steatorrhea - ✔✔> 60 droplets/hpf (neutral fats)
Split fat - ✔✔both number and size of fat droplets must be considered
3
©JOSHCLAY 2024/2025. YEAR PUBLISHED 2024.
