BIOCHEM 4300 FINAL EXAM QUESTIONS WITH CORRECT
ANSWERS 100% VERIFIED
spin status - ANSWER nuclear magnetic radiation (NMR) occurs when the nucleus
changes its ___
differences in T1 and T2 radiation - ANSWER in MRI, tissue contrast is determined by
differences in ___
scalar coupling - ANSWER mediated through chemical bonds connecting two spins
connectivity - ANSWER scalar coupling provides ___ information
dipolar coupling - ANSWER mediated by two NMR active nuclei through space
spatial - ANSWER dipolar coupling provides ___ information
correlation spectroscopy - ANSWER used to identify amino acid side chain spin systems
triple resonance spectroscopy - ANSWER used to identify neighboring residues
NMR linewidth - ANSWER at full peak when 1/2 maximum
narrow linewidth - ANSWER faster motion
wider linewidth - ANSWER slower motion
, linewidth - ANSWER dependent on molecular weight and structure
fragments - ANSWER have narrower linewidths
slow exchange - ANSWER peaks don't shift. just appear/disappear
fast exchange - ANSWER single peak at weighted average position
alpha helix circular dichromism - ANSWER 208 and 222 nm
beta sheet circular dichromism - ANSWER 217 nm
fluorescence - ANSWER the radiative return of an excited electronic state to the ground
electronic state
fluorescence processes - ANSWER absorption, internal conversion, release of a photon
stokes shift - ANSWER separation between excitation and emission maxima
tryptophan - ANSWER can quench signal from PHE and TYR
biomolecular fluorescence complementation (BiFC) - ANSWER detects protein protein
interactions
probability of FRET energy transfer - ANSWER depends on overlap of donor emission
spectrum and acceptor absorption spectrum
magnetic fields - ANSWER electron microscopes use ___ to focus beam
ANSWERS 100% VERIFIED
spin status - ANSWER nuclear magnetic radiation (NMR) occurs when the nucleus
changes its ___
differences in T1 and T2 radiation - ANSWER in MRI, tissue contrast is determined by
differences in ___
scalar coupling - ANSWER mediated through chemical bonds connecting two spins
connectivity - ANSWER scalar coupling provides ___ information
dipolar coupling - ANSWER mediated by two NMR active nuclei through space
spatial - ANSWER dipolar coupling provides ___ information
correlation spectroscopy - ANSWER used to identify amino acid side chain spin systems
triple resonance spectroscopy - ANSWER used to identify neighboring residues
NMR linewidth - ANSWER at full peak when 1/2 maximum
narrow linewidth - ANSWER faster motion
wider linewidth - ANSWER slower motion
, linewidth - ANSWER dependent on molecular weight and structure
fragments - ANSWER have narrower linewidths
slow exchange - ANSWER peaks don't shift. just appear/disappear
fast exchange - ANSWER single peak at weighted average position
alpha helix circular dichromism - ANSWER 208 and 222 nm
beta sheet circular dichromism - ANSWER 217 nm
fluorescence - ANSWER the radiative return of an excited electronic state to the ground
electronic state
fluorescence processes - ANSWER absorption, internal conversion, release of a photon
stokes shift - ANSWER separation between excitation and emission maxima
tryptophan - ANSWER can quench signal from PHE and TYR
biomolecular fluorescence complementation (BiFC) - ANSWER detects protein protein
interactions
probability of FRET energy transfer - ANSWER depends on overlap of donor emission
spectrum and acceptor absorption spectrum
magnetic fields - ANSWER electron microscopes use ___ to focus beam
