MOLECULAR DIAGNOSTICS (THE
MOLECULAR DIAGNOSTICS LECTURE
FOR 2ND YEAR MLT PROGRAM AT
PCC)
Define Molecular Diagnostics - ANSWER-Use of DNA, RNA and protein aka amino
acid, to test for specific disease
What is one of the way molecular diagnostic are use in the lab and gave an example? -
ANSWER-To speed up the identification of
1) slow growing microrganism
2) Fastidious microrganisms
3) Microrganism that wont grow on conventional media>
What are the Molecular diagnostic Principle and describle each one? - ANSWER-1)
Target sequence, which is to target the a base sequence for that a single specific
mircoorganism.
2) Amplification which is the replication of the target sequence that we are after.
3) Detection is use to detect that we have the right sequence after all.
Describe DNA transcription - ANSWER-The process through which DNA sequence is
enzymatically copied by RNA polymerase to produce a complemetary RNA, aka
messager RNA (mRNA)
Describe the structure of DNA. - ANSWER-1) It is a double helix
2) Sugar
3) Phosphate group
4) Base
What are the base in a nucleotide? Which one are Purine and which ones are
Pyrimidines? - ANSWER-1) Purine are Adenine, and Guaine
2) Pyrimidines are Cytosine and Thymine
What are the bond between
1) nucleotides
2) 2 strands of DNA - ANSWER-1) Phosphodiester, which are strong covalent bond
2) Hydrogen bonds which are unstable.
What does it mean by the DNA are Antiparallel nature? - ANSWER-It means that there
is one strand that read form 5' to 3' and second strand read form 3'to 5'.
MOLECULAR DIAGNOSTICS LECTURE
FOR 2ND YEAR MLT PROGRAM AT
PCC)
Define Molecular Diagnostics - ANSWER-Use of DNA, RNA and protein aka amino
acid, to test for specific disease
What is one of the way molecular diagnostic are use in the lab and gave an example? -
ANSWER-To speed up the identification of
1) slow growing microrganism
2) Fastidious microrganisms
3) Microrganism that wont grow on conventional media>
What are the Molecular diagnostic Principle and describle each one? - ANSWER-1)
Target sequence, which is to target the a base sequence for that a single specific
mircoorganism.
2) Amplification which is the replication of the target sequence that we are after.
3) Detection is use to detect that we have the right sequence after all.
Describe DNA transcription - ANSWER-The process through which DNA sequence is
enzymatically copied by RNA polymerase to produce a complemetary RNA, aka
messager RNA (mRNA)
Describe the structure of DNA. - ANSWER-1) It is a double helix
2) Sugar
3) Phosphate group
4) Base
What are the base in a nucleotide? Which one are Purine and which ones are
Pyrimidines? - ANSWER-1) Purine are Adenine, and Guaine
2) Pyrimidines are Cytosine and Thymine
What are the bond between
1) nucleotides
2) 2 strands of DNA - ANSWER-1) Phosphodiester, which are strong covalent bond
2) Hydrogen bonds which are unstable.
What does it mean by the DNA are Antiparallel nature? - ANSWER-It means that there
is one strand that read form 5' to 3' and second strand read form 3'to 5'.
