MOLECULAR BIOLOGY EXAM 1 STUDY
GUIDE WITH COMPLETE SOLUTIONS
In the 1944 experiment by Avery et al., only ___ was able to transform a nonvirulent
strain of bacteria into a virulent one, thereby demonstration that it is likely the ___.
A) protein; chromosomal material
B) genetics; protein material
C) amino acid; DNA material
D) DNA; genetic material - ANSWER-D) DNA; genetic material
Hershey and Chase (1952) blender experiment demonstrated that
A) protein is not required for virus production
B) viral coat proteins can enter the infected bacterial cells
C) Reverse transcription is important for viral infection
D) DNA carries the genetic information for viral reproduction - ANSWER-D) DNA carries
the genetic information for viral reproduction
If a DNA fragment contains 42 base pairs, which of the following is true?
A) the DNA length is 3.8 nm
B) DNA has 84 hydrogen bonds
C) the DNA has four minor grooves and four major grooves
D) the diameter of the DNA is 22 angstroms - ANSWER-C) the DNA has four minor
grooves and four major grooves
In a piece of DNA which of the following is true?
A) A+T = G+C
B) A+G = C+T
C) A=C
D) Both A and B - ANSWER-B) A+G = T+C
Which of the following statements is false?
A) the two strands of a DNA helix are identical in sequence
B) the two strands of a DNA helix are identical in base numbers
C) the two strands of a DNA helix are identical in length
D) the two strands of a DNA helix are identical in hydrogen bond numbers - ANSWER-
A) the two strands of a DNA helix are identical in sequence
The following proportions exist in DNA: C=G, A=T
A) Yes, this is Chargaff's Rule
B) No, it is A=C, G=T
C) False, C>G, A>T
D) None of the above are correct - ANSWER-A) Yes this is Chargaff's Rule
, H2A and H3 histones in humans
A) both contain N-terminal tails that can be chemically modified
B) are small and rich in arginines and lysines
C) are very similar in amino acid sequence to H2A and H3 in horses
D) all of the above - ANSWER-D) all of the above
The most common DNA binding domain found in proteins is the
A) helix-turn-helix
B) dimerization domains
C) beta-sheet
D) CAP domain - ANSWER-A) helix-turn-helix
Which of the reasons listed below explains the ability of RNA to fold into more complex
structures than DNA?
A) RNA has a second -OH group when compared to DNA
B) RNA contains uracil, which can pair to both A and G
C) unlike DNA< RNA can form triple base pairs
D) All of the above - ANSWER-D) All of the above
In a beta-sheet, the R groups of the amino acid residues
A) form an alpha-helix
B) are found poking out of the top and bottom of the sheet
C) cause only parallel sheets to form
D) generate the hydrogen bonds that form the sheet - ANSWER-B) are found poking
out of the top and bottom of the sheet
Chromodomains bing:
A) acetylated histones
B) methylated histones
C) only non-acetylated and non-methylated histones
D) phosphorylated histones - ANSWER-B) methylated histones
The major groove of a DNA double helix
A) Presents a wide variety of chemical groups to proteins
B) Is not much different from the minor groove
C) Is distinctive in prokaryotes but not eukaryotes
D) Is the site of histone/DNA interactions - ANSWER-A) Presents a wide variety of
chemical groups to proteins
In a DNA chain, the nitrogenous base is attached to the ___ carbon of ribose
A) 4'
B) 3'
C) 2'
D) 1' - ANSWER-D) 1'
Which of the following statements are true with regard to protein-DNA interactions?
GUIDE WITH COMPLETE SOLUTIONS
In the 1944 experiment by Avery et al., only ___ was able to transform a nonvirulent
strain of bacteria into a virulent one, thereby demonstration that it is likely the ___.
A) protein; chromosomal material
B) genetics; protein material
C) amino acid; DNA material
D) DNA; genetic material - ANSWER-D) DNA; genetic material
Hershey and Chase (1952) blender experiment demonstrated that
A) protein is not required for virus production
B) viral coat proteins can enter the infected bacterial cells
C) Reverse transcription is important for viral infection
D) DNA carries the genetic information for viral reproduction - ANSWER-D) DNA carries
the genetic information for viral reproduction
If a DNA fragment contains 42 base pairs, which of the following is true?
A) the DNA length is 3.8 nm
B) DNA has 84 hydrogen bonds
C) the DNA has four minor grooves and four major grooves
D) the diameter of the DNA is 22 angstroms - ANSWER-C) the DNA has four minor
grooves and four major grooves
In a piece of DNA which of the following is true?
A) A+T = G+C
B) A+G = C+T
C) A=C
D) Both A and B - ANSWER-B) A+G = T+C
Which of the following statements is false?
A) the two strands of a DNA helix are identical in sequence
B) the two strands of a DNA helix are identical in base numbers
C) the two strands of a DNA helix are identical in length
D) the two strands of a DNA helix are identical in hydrogen bond numbers - ANSWER-
A) the two strands of a DNA helix are identical in sequence
The following proportions exist in DNA: C=G, A=T
A) Yes, this is Chargaff's Rule
B) No, it is A=C, G=T
C) False, C>G, A>T
D) None of the above are correct - ANSWER-A) Yes this is Chargaff's Rule
, H2A and H3 histones in humans
A) both contain N-terminal tails that can be chemically modified
B) are small and rich in arginines and lysines
C) are very similar in amino acid sequence to H2A and H3 in horses
D) all of the above - ANSWER-D) all of the above
The most common DNA binding domain found in proteins is the
A) helix-turn-helix
B) dimerization domains
C) beta-sheet
D) CAP domain - ANSWER-A) helix-turn-helix
Which of the reasons listed below explains the ability of RNA to fold into more complex
structures than DNA?
A) RNA has a second -OH group when compared to DNA
B) RNA contains uracil, which can pair to both A and G
C) unlike DNA< RNA can form triple base pairs
D) All of the above - ANSWER-D) All of the above
In a beta-sheet, the R groups of the amino acid residues
A) form an alpha-helix
B) are found poking out of the top and bottom of the sheet
C) cause only parallel sheets to form
D) generate the hydrogen bonds that form the sheet - ANSWER-B) are found poking
out of the top and bottom of the sheet
Chromodomains bing:
A) acetylated histones
B) methylated histones
C) only non-acetylated and non-methylated histones
D) phosphorylated histones - ANSWER-B) methylated histones
The major groove of a DNA double helix
A) Presents a wide variety of chemical groups to proteins
B) Is not much different from the minor groove
C) Is distinctive in prokaryotes but not eukaryotes
D) Is the site of histone/DNA interactions - ANSWER-A) Presents a wide variety of
chemical groups to proteins
In a DNA chain, the nitrogenous base is attached to the ___ carbon of ribose
A) 4'
B) 3'
C) 2'
D) 1' - ANSWER-D) 1'
Which of the following statements are true with regard to protein-DNA interactions?
