CULTURING MICROORGANISMS EXAM QUESTIONS WITH VERIFIED ANSWERS LATEST UPDATE 2024
(GRADED A+)
Culturing Microorganisms - Answers -Inoculation
-Isolation
-Inspection
-Incubation
-Identification
Fresh - Answers Light microscopy
Bright field microscopy - Answers Fixed, stained smears
Phase contrast and inference microscopy - Answers Negative positive
Simple staining - Answers Fluorescence microscopy
serologic reactions are - Answers antigen-antibody reactions
inoculation, incubation, isolation, inspection and identification - Answers The five (i)
Metabolism includes - Answers enzymes (catalase, oxidase)
phage typing - Answers using bacteriophages to identify bacterial strains
DNA hybridization - Answers to determine closeness of relationship between various bacteria
Microscopic morphology includes a combination of - Answers cell shape, size, Gram's stain, acid fast rxn,
special structures
endospores, granule, flagella and capsule can be used to - Answers give an initial presumptive
identification.
INOCULATION - Answers to culture microorganisms a tiny sample (inoculum) is introduced into medium
(inoculation).
once the media is inoculated it is incubated which means putting the culture in a controlled
environment to allow for multiplication - Answers INCUBATION
ISOLATION - Answers separating one species from others to get a pure culture
INSPECTED and IDENTIFIED - Answers phenotypically, immunologically or genetically.
Phenotypic methods - Answers media (enriched, selective, differential, characteristic), direct
microscopic examination, staining, biochemical tests, rapid tests, bacteriophage typing, flow cytometry.
, precipitation, agglutination reactions, fluorescent antibodies, ELISA, immunoblot/western blot. -
Answers Immunological methods
nucleic acid probes, PCR, nucleic acid sequencing, RFLP, plasmids fingerprinting. - Answers Genotypic
methods involve examining the genetic material of the organisms
-Using the proper aseptic techniques.
-Correctly obtaining the specimen.
-Correctly handling the specimen
-Quickly transporting the specimen to the lab.
-Once the specimen reaches the lab it is cultured and identified. - Answers successful identification of
microbe
collected, handled and stored - Answers Successful identification depends on how the specimen is
Phenotypic methods - Answers Growth on enrichment, selective, differential or characteristic media
(a combination of cell shape, size, Gram's stain, acid fast rxn, special structures e.g. endospores, granule,
flagella and capsule) - Answers Phenotypic methods of Microscopy and Staining includes
Phenotypic methods - Answers Specimen biochemical test (rapid test methods)
Morphology - Answers cell shape and size
Metabolism is a - Answers biochemical reaction
gram staining reaction - Answers -staining reactions
including colony size, shape, and colour - Answers growth characteristics
environmental requirements - Answers O2 tolerance, temp. tolerance
old-fashioned - Answers methods via biochemical, serological and morphological
macroscopic morphology (phenotypic) traits - Answers can be accessed with the naked eye
examples of macroscopic morphology (phenotypic) - Answers appearance of colony including texture,
shape, pigment, speed of growth and growth pattern in broth.
Physiology/Biochemical characteristic are - Answers enzymes (catalase, oxidase), fermentation of
sugars, capacity to digest or metabolize complex polymers and sensitivity to drugs can be used in
identification.
Interaction of a microbial antigen with an antibody - Answers Immunological methods involve
(GRADED A+)
Culturing Microorganisms - Answers -Inoculation
-Isolation
-Inspection
-Incubation
-Identification
Fresh - Answers Light microscopy
Bright field microscopy - Answers Fixed, stained smears
Phase contrast and inference microscopy - Answers Negative positive
Simple staining - Answers Fluorescence microscopy
serologic reactions are - Answers antigen-antibody reactions
inoculation, incubation, isolation, inspection and identification - Answers The five (i)
Metabolism includes - Answers enzymes (catalase, oxidase)
phage typing - Answers using bacteriophages to identify bacterial strains
DNA hybridization - Answers to determine closeness of relationship between various bacteria
Microscopic morphology includes a combination of - Answers cell shape, size, Gram's stain, acid fast rxn,
special structures
endospores, granule, flagella and capsule can be used to - Answers give an initial presumptive
identification.
INOCULATION - Answers to culture microorganisms a tiny sample (inoculum) is introduced into medium
(inoculation).
once the media is inoculated it is incubated which means putting the culture in a controlled
environment to allow for multiplication - Answers INCUBATION
ISOLATION - Answers separating one species from others to get a pure culture
INSPECTED and IDENTIFIED - Answers phenotypically, immunologically or genetically.
Phenotypic methods - Answers media (enriched, selective, differential, characteristic), direct
microscopic examination, staining, biochemical tests, rapid tests, bacteriophage typing, flow cytometry.
, precipitation, agglutination reactions, fluorescent antibodies, ELISA, immunoblot/western blot. -
Answers Immunological methods
nucleic acid probes, PCR, nucleic acid sequencing, RFLP, plasmids fingerprinting. - Answers Genotypic
methods involve examining the genetic material of the organisms
-Using the proper aseptic techniques.
-Correctly obtaining the specimen.
-Correctly handling the specimen
-Quickly transporting the specimen to the lab.
-Once the specimen reaches the lab it is cultured and identified. - Answers successful identification of
microbe
collected, handled and stored - Answers Successful identification depends on how the specimen is
Phenotypic methods - Answers Growth on enrichment, selective, differential or characteristic media
(a combination of cell shape, size, Gram's stain, acid fast rxn, special structures e.g. endospores, granule,
flagella and capsule) - Answers Phenotypic methods of Microscopy and Staining includes
Phenotypic methods - Answers Specimen biochemical test (rapid test methods)
Morphology - Answers cell shape and size
Metabolism is a - Answers biochemical reaction
gram staining reaction - Answers -staining reactions
including colony size, shape, and colour - Answers growth characteristics
environmental requirements - Answers O2 tolerance, temp. tolerance
old-fashioned - Answers methods via biochemical, serological and morphological
macroscopic morphology (phenotypic) traits - Answers can be accessed with the naked eye
examples of macroscopic morphology (phenotypic) - Answers appearance of colony including texture,
shape, pigment, speed of growth and growth pattern in broth.
Physiology/Biochemical characteristic are - Answers enzymes (catalase, oxidase), fermentation of
sugars, capacity to digest or metabolize complex polymers and sensitivity to drugs can be used in
identification.
Interaction of a microbial antigen with an antibody - Answers Immunological methods involve
