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Holechek Bio 340 Exam 3/ 53 Quizzes with Certified Ans/ Already Graded A+.

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Holechek Bio 340 Exam 3/ 53 Quizzes with Certified Ans/ Already Graded A+. This technique uses an extremophile in order to replicate DNA quickly. - Answer: PCR The first thing that happens to DNA during the PCR process is ____? - Answer: denaturation The second thing that happens during the PCR process is ____? - Answer: primer annealing phase in PCR during which a primer binds to a template strand - Answer: primer annealing The final step of the PCR process is termed ____? - Answer: extension what type of electric charge does DNA have? - Answer: negative Other than charge what is a crucial component of DNA during gel electrophoresis? - Answer: size Short DNA fragments that can be stained with something such as a radioactive dye in order to further understand DNA are called - Answer: Probes Transfer of replicated DNA from a donor to a recipient - Answer: Conjugation In bacteria, the direct transfer of DNA between two cells that are temporarily joined. - Answer: Conjugation What important structure is used to transfer replicated DNA during conjugation - Answer: Pilus Where does the DNA come from during transformation? - Answer: enviroment

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December 7, 2024
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Holechek Bio 340 Exam 3/ 53 Quizzes with
Certified Ans/ Already Graded A+.
This technique uses an extremophile in order to replicate DNA quickly. - Answer: PCR


The first thing that happens to DNA during the PCR process is ____? - Answer: denaturation


The second thing that happens during the PCR process is ____? - Answer: primer annealing


phase in PCR during which a primer binds to a template strand - Answer: primer annealing


The final step of the PCR process is termed ____? - Answer: extension


what type of electric charge does DNA have? - Answer: negative


Other than charge what is a crucial component of DNA during gel electrophoresis? - Answer: size


Short DNA fragments that can be stained with something such as a radioactive dye in order to further
understand DNA are called - Answer: Probes


Page 1 of 7

, Transfer of replicated DNA from a donor to a recipient - Answer: Conjugation


In bacteria, the direct transfer of DNA between two cells that are temporarily joined. - Answer:
Conjugation


What important structure is used to transfer replicated DNA during conjugation - Answer: Pilus


Where does the DNA come from during transformation? - Answer: enviroment


Transformation can be done with DNA fragments OR a DNA ____ - Answer: Plasmid


DNA is transferred from a donor cell to a recipient via a bacteriophage - Answer: Transduction


Transfer of DNA from one bacterium to another using a viral vector is called ____ - Answer: Transduction


Sometimes plasmids can cause problems when they contain harmful information because when treating
the patient its found these plasmids have ____ ____( two words) - Answer: antibiotic resistance


region in plasmid that has been engineered to contain recognition sites of a number of restriction
endonucleases - Answer: multiple cloning site


The most common reporter gene used in plasmids is the ___ _ operon - Answer: Lac Z


During white/blue screening which of the two contains the recombinant plasmids? - Answer: white


Bacteriophages look like a creepy creature out of what film series featuring keanu reeves? - Answer: The
matrix


reading DNA ends up being the same backwards and forwards earns the DNA the descriptive term ____ -
Answer: palindromic



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