Microbiology LAB Exam -1 Questions And Answers.

Microbiology LAB Exam #1 Questions
And Answers.



Microscope- The Condenser - (correct answer) -focuses the light ging into the
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objective lens
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Microscope- The Iris Diaphragm - (correct answer) -adjusts the amount of light
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going into the objective lens
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Microscope- Nosepiece - (correct answer) -holds the objective lenses and rotates
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to change from one objective to another
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Microscope- Coarse Objective Knob - (correct answer) -moves the stage larger
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distances to help get the object in focus
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Microscope- Fine Adjustment Knob - (correct answer) -moves the stage smaler
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increments to help get the object in better focus
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Microscope- Objective Lens - (correct answer) -magnifies the object. It is located
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near the object.
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Microscope- Occular Lens - (correct answer) -magnifies the image of the object
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from the objective lens. It is located near the eye.
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Steps in using the microscope - (correct answer) -1) scanner power in place
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2) place slide of specimen stage in place with stage clips
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3) Using the course adjustment knob, raise the stage all the way up and then
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down slowly until you can see image of specimen
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4) Readjust with fine adjustment knob
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Describe the purpose of the oil immersion lens - (correct answer) -Used when oil
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is added to the slide. Oil must touch the object and objective lens to that the
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light does not refract futher
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, Parfocal - (correct answer) -Parfocal microscopes are able to move from one
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objective to the next and still be in focus due to the length of the objective
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lenses
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Resolution - (correct answer) -The ability to distinguish between two objects that
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are very close together.
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What is resolving power? - (correct answer) -The measurement of resolution
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What are two ways to increase resolving power? - (correct answer) -1. By
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decreasing the wavelength of light (using a blue filter) 2. Increasing the
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numerical aperature
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What units are used to express resolving power? - (correct answer) -nm or
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nanometers
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What is better, smaller or larger resolving power? - (correct answer) -Smaller is
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better
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Light microscopes have a 200nm resolving power
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Electron microscopes have a .2nm resolving power
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What is chromatic aberration? - (correct answer) -The effect when many colors
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are seen toward the edge of the microscope field of vision (like a prisim)
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How are microscopes corrected for chromatic aberration? - (correct answer) -
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With a special achromatic lens (corrected for red and blue light) or by using a
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blue filter. The lab has blue filters in their microscopes which also increase
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resolution.
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Describe the process of making a wet-mount slide - (correct answer) -1) Add the
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specimen to the slide
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2) Touch the edge of the cover slip with one edge of drop of specimen.
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3) Let the cover slip drop gradually to the slide, covering the drop of specimen.
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Describe the purpose of making a wet-mount slide - (correct answer) -To be able
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to see living organisms under the microscope in their natural state.
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