And Answers
Heat reaction to 95 C to denature DNA
ml ml ml ml ml ml ml
Cool rxn to 55 C to allow primers to anneal
ml ml ml ml ml ml ml ml ml
Heat rxn to 72 C to allow Taq polymerase to bind - (correct answer) -Give the
ml ml ml ml ml ml ml ml ml ml ml ml ml ml ml
steps of a PCR
ml ml ml ml
thermocycler - (correct answer) -What machine is used to carry out a PCR
ml ml ml ml ml ml ml ml ml ml ml ml
reaction?
ml
30 - (correct answer) -How many cycles does a PCR rxn usually go?
ml ml ml ml ml ml ml ml ml ml ml ml
Bacillus subtillis ml
Enterobacter aerogenes ml
Entero cloacae ml
Escherichia coli ml
Klebsiella pneumoniae ml
Pseudomonas aeruginosa ml
Salmonella typhimurium ml
Staphylococcus aureus ml
Staphylococcus epidermidis - (correct answer) -Name the 9 organisms used in ml ml ml ml ml ml ml ml ml ml
the unknown lab
ml ml ml
E. coli
ml
E. cloacae
ml
E. aerogenes
ml
, K. pneumonia - (correct answer) -Which organisms can ferment lactose?
ml ml ml ml ml ml ml ml ml
rod-shaped, Gram-negative, non-spore forming, and motile or non-motile
ml ml ml ml ml ml ml
bacteria which can ferment lactose with the production of acid and gas -
ml ml ml ml ml ml ml ml ml ml ml ml ml
(correct answer) -Define caliform bacteria
ml ml ml ml ml
Blood Agar ml
Bile Esculin ml
Mannitol Salt Agar (MSA) ml ml ml
MacConkey Agar (MaC) ml ml
Eosin Methylene Blue (EMB)
ml ml ml
Hektoen Enteric Agar (HEA) ml ml ml
Xylose Lysine Desoxiolate (XLD) - (correct answer) -Name the selective medias
ml ml ml ml ml ml ml ml ml ml
used in class
ml ml ml
O/F Glucose ml
MRVP
Catalase
Oxidase
Citrate
Starch
Indole
TSI - (correct answer) -Name the differential medias used in class
ml ml ml ml ml ml ml ml ml ml
indole
methyl red ml
vogues proskaver ml
in
citrate - (correct answer) -What does the acronym IMViC stand for?
ml ml ml ml ml ml ml ml ml ml