Intro to Molecular Cell Biology Test Bank Chapter 6
The process of DNA replica on requires that each of the parental DNA strands be used as a
____________ to produce a duplicate of the opposing strand.
(a) catalyst
(b) compe tor
(c) template
(d) copy - (c) template
DNA replica on is considered semiconserva ve because __________________________________.
(a) a er many rounds of DNA replica on, the original DNA double helix is s ll intact.
(b) each daughter DNA molecule consists of two new strands copied form the parent
(c) each daughter DNA molecule consists of one strand from the parent DNA molecule and one new
strand. - (c) each daughter DNA molecule consists of one strand from the parent DNA molecule and one
new strand
The classic experiment conducted by Meselson and Stahl demonstrated that DNA replica on is
accomplished by employing a ______________ mechanism. - semiconserva ve
Ini ator proteins bind to replica on origins and disrupt hydrogen bonds between the two DNA strands
being copied. Which of the factors below does not contribute to the rela ve ease of strand separa on by
ini ator proteins?
(a) replica on origins are rich in A-T base pairs
(b) the reac on can occur at room temperature
(c) they only separate a few base pairs at a me
(d) once opened, other por ons of the DNA replica on machinery bind to the origin - (b) the reac on
can occur at room temperature
If the genome of the bacterium E. coli requires about 20 minutes to replicate itself, how can the genome
of a fruit fly be replicated in only 3 minutes?
(a) The fruit fly genome is smaller than E coli
, (b) Eukaryo c DNA polymerase synthesizes DNA at a much faster rate than prokaryo c
(c) The nuclear membrane keeps the fruit fly DNA concentrated in one place in the cell, which increases
the rate of polymeriza on
(d) Fruit fly DNA contains more origins of replica on than E coli DNA. - (d) Fruit fly DNA contains more
origins of replica on than E coli DNA
True or false?
When DNA is being replicated inside a cell, local hea ng occurs, allowing the two strands to separate. -
False. This is accomplished by ini ated proteins binding at the origin of replica on.
True or false?
DNA replica on origins are typically rich in G-C base pairs. - They are rich in A-T base pairs which makes
it easier for them to separate the strands because they are only held together by two hydrogen bonds
True or false?
Meselson and Stahl ruled out the dispersive model for DNA replica on. - true
True or false?
DNA replica on is a bidirec onal process that is ini ated at mul ple loca ons among chromosomes in
eukaryo c cells. - True
How many replica on forks are formed when an origin of replica on is opened? - 2
How does the total number of replica on origins in bacterial cells compare with the number of origins in
human cells? - 1 versus 10,000
Which of the following statement correctly explains what it means for DNA replica on to be
bidirec onal?
(a) The replica on forks formed at the origin moved in opposite direc ons.
(b) the DNA replica on machinery can move in either direc on on the template strand
(c) The replica on fork can either open or close depending on the condi on
The process of DNA replica on requires that each of the parental DNA strands be used as a
____________ to produce a duplicate of the opposing strand.
(a) catalyst
(b) compe tor
(c) template
(d) copy - (c) template
DNA replica on is considered semiconserva ve because __________________________________.
(a) a er many rounds of DNA replica on, the original DNA double helix is s ll intact.
(b) each daughter DNA molecule consists of two new strands copied form the parent
(c) each daughter DNA molecule consists of one strand from the parent DNA molecule and one new
strand. - (c) each daughter DNA molecule consists of one strand from the parent DNA molecule and one
new strand
The classic experiment conducted by Meselson and Stahl demonstrated that DNA replica on is
accomplished by employing a ______________ mechanism. - semiconserva ve
Ini ator proteins bind to replica on origins and disrupt hydrogen bonds between the two DNA strands
being copied. Which of the factors below does not contribute to the rela ve ease of strand separa on by
ini ator proteins?
(a) replica on origins are rich in A-T base pairs
(b) the reac on can occur at room temperature
(c) they only separate a few base pairs at a me
(d) once opened, other por ons of the DNA replica on machinery bind to the origin - (b) the reac on
can occur at room temperature
If the genome of the bacterium E. coli requires about 20 minutes to replicate itself, how can the genome
of a fruit fly be replicated in only 3 minutes?
(a) The fruit fly genome is smaller than E coli
, (b) Eukaryo c DNA polymerase synthesizes DNA at a much faster rate than prokaryo c
(c) The nuclear membrane keeps the fruit fly DNA concentrated in one place in the cell, which increases
the rate of polymeriza on
(d) Fruit fly DNA contains more origins of replica on than E coli DNA. - (d) Fruit fly DNA contains more
origins of replica on than E coli DNA
True or false?
When DNA is being replicated inside a cell, local hea ng occurs, allowing the two strands to separate. -
False. This is accomplished by ini ated proteins binding at the origin of replica on.
True or false?
DNA replica on origins are typically rich in G-C base pairs. - They are rich in A-T base pairs which makes
it easier for them to separate the strands because they are only held together by two hydrogen bonds
True or false?
Meselson and Stahl ruled out the dispersive model for DNA replica on. - true
True or false?
DNA replica on is a bidirec onal process that is ini ated at mul ple loca ons among chromosomes in
eukaryo c cells. - True
How many replica on forks are formed when an origin of replica on is opened? - 2
How does the total number of replica on origins in bacterial cells compare with the number of origins in
human cells? - 1 versus 10,000
Which of the following statement correctly explains what it means for DNA replica on to be
bidirec onal?
(a) The replica on forks formed at the origin moved in opposite direc ons.
(b) the DNA replica on machinery can move in either direc on on the template strand
(c) The replica on fork can either open or close depending on the condi on
