©FYNDLAY EXAM SOLUTIONS 2024/2025
ALL RIGHTS RESERVED.
ACS Biochemistry First Semester Exam
Questions And Answers (Guaranteed A+)
Buffer Order and Henderson Hasselbach - answer✔Water, Buffer, Salt, Base
pH = pKa + log(A/HA)
pKa decrease as temp increase so pH decrease
Ramachandran plots - answer✔Φ = N-R
ψ = C-R
optimal angle to prevent steric hinderance
Protein structure of a helix, B sheet, reverse turn - answer✔a: LEMARKHQ
B: VICYFTW
RT: PSDNG
how many amino acids in alpha turn helix - answer✔3.6
Super secondary structure - answer✔helix turn helix
parallel vs. antiparallel - answer✔parallel wedges face same way
antiparallel face opposite
Isoelectric point (pI) - answer✔pH at which a particular molecule carries no net electrical
charge.
Protein Separation Techniques (ALL) - answer✔Homogenization
Salting Out
Affinity Chromatgraphy
Ion Exchange
Size Exclusion/Gel Filtration
1|Page
, ©FYNDLAY EXAM SOLUTIONS 2024/2025
ALL RIGHTS RESERVED.
H, S, A, I, SG
He said all individuals SinG
Protein Separation Techniques (H) - answer✔Homogenization
disrupt cells, sonicator, centrifuge
Protein Separation Techniques (S) - answer✔Salting out
different proteins precipitate out at different salt concentrations
Protein Separation Techniques (A) - answer✔Affinity chromatography
add a tag; histidine binds; see how well there is an affinity for the group
Protein Separation Techniques (I) - answer✔ion exchange
separation based on charge; + proteins stick to - beads
Protein Separation Techniques (SG) - answer✔Size Exclusion/Gel Filtration
separation based on size
Cuts (protein separation techniques) - answer✔cut out what dont need/isnt protein
(use 40% concen. then keep liquid; if use 60% concen. then keep pellet)
Dialysis - answer✔protein separation based on bad with pores
1. Moles in bag + moles in buffer
2. Divide moles by total volume - new concentration
Electrophoresis - answer✔gels - move by size with electric current
1. Isoelectric focusing - purely pH
2. SDS page
pH separation is by pI and charge in 2d
Protein sequencing (who cuts after what) - answer✔Cyanogen bromide = methionine
trypsin = K, and R
chymotrypsin = F, M, L, W, Y
IP - answer✔Immunoprecipitation with proteins
1. add antibodies specific to protein of interest to lysed cells
2|Page
ALL RIGHTS RESERVED.
ACS Biochemistry First Semester Exam
Questions And Answers (Guaranteed A+)
Buffer Order and Henderson Hasselbach - answer✔Water, Buffer, Salt, Base
pH = pKa + log(A/HA)
pKa decrease as temp increase so pH decrease
Ramachandran plots - answer✔Φ = N-R
ψ = C-R
optimal angle to prevent steric hinderance
Protein structure of a helix, B sheet, reverse turn - answer✔a: LEMARKHQ
B: VICYFTW
RT: PSDNG
how many amino acids in alpha turn helix - answer✔3.6
Super secondary structure - answer✔helix turn helix
parallel vs. antiparallel - answer✔parallel wedges face same way
antiparallel face opposite
Isoelectric point (pI) - answer✔pH at which a particular molecule carries no net electrical
charge.
Protein Separation Techniques (ALL) - answer✔Homogenization
Salting Out
Affinity Chromatgraphy
Ion Exchange
Size Exclusion/Gel Filtration
1|Page
, ©FYNDLAY EXAM SOLUTIONS 2024/2025
ALL RIGHTS RESERVED.
H, S, A, I, SG
He said all individuals SinG
Protein Separation Techniques (H) - answer✔Homogenization
disrupt cells, sonicator, centrifuge
Protein Separation Techniques (S) - answer✔Salting out
different proteins precipitate out at different salt concentrations
Protein Separation Techniques (A) - answer✔Affinity chromatography
add a tag; histidine binds; see how well there is an affinity for the group
Protein Separation Techniques (I) - answer✔ion exchange
separation based on charge; + proteins stick to - beads
Protein Separation Techniques (SG) - answer✔Size Exclusion/Gel Filtration
separation based on size
Cuts (protein separation techniques) - answer✔cut out what dont need/isnt protein
(use 40% concen. then keep liquid; if use 60% concen. then keep pellet)
Dialysis - answer✔protein separation based on bad with pores
1. Moles in bag + moles in buffer
2. Divide moles by total volume - new concentration
Electrophoresis - answer✔gels - move by size with electric current
1. Isoelectric focusing - purely pH
2. SDS page
pH separation is by pI and charge in 2d
Protein sequencing (who cuts after what) - answer✔Cyanogen bromide = methionine
trypsin = K, and R
chymotrypsin = F, M, L, W, Y
IP - answer✔Immunoprecipitation with proteins
1. add antibodies specific to protein of interest to lysed cells
2|Page
