Bio 150 Lab Midterm Question and
answers verified to pass (34 pgs)
lab week 1-3 - correct answer ✔develop question
lab week 2-5 - correct answer ✔write project proposal
lab week 3-6 - correct answer ✔collect samples for DNA barcoding
lab week 6-10 - correct answer ✔extract dna, pcr, and barcode samples
lab week 10-11 - correct answer ✔write up results
lab week 12-14 - correct answer ✔write discussion and abstract
what is a grant proposal? - correct answer ✔major source of funding for
projects, required proposal in order to receive money
According to your lab manual, the two main factors that contribute to the
quality of your DNA extracts are DNA purity and PCR inhibitors - correct
answer ✔False
True or False: Ethanol, proteins, lipids in a DNA extract can potentially inhibit
PCR reactions (cause them to fail). - correct answer ✔True
This number can indicate the concentration of DNA in your DNA extract -
correct answer ✔A260
,peak wavelength of light DNA absorbs - correct answer ✔260nm
peak wavelength of light proteins absorb - correct answer ✔280nm
peak wavelength of light salts absorb - correct answer ✔230nm
peak wavelength of light carbohydrates absorb - correct answer ✔230nm
ideal DNA concentration (ng/uL) - correct answer ✔100
ideal A260:280 - correct answer ✔1.8
ideal A260:230 - correct answer ✔2.2
A260:230 ratio, below which indicates potentially significant contamination -
correct answer ✔1.4
A260:280 ratio, below which indicates potentially significant contamination -
correct answer ✔1.4
goal DNA concentration for extraction - correct answer ✔50ng/ul-250ng/ul
After running your DNA extract through a nanodrop spectrophotometer you
find that your extract has a DNA concentration of 0.1 ng/uL and your
A260:280 is 1.80, which of the following should you do to increase the
likelihood of your DNA extract PCR amplifying correctly? - correct answer
✔You should do another extraction, your DNA concentration is too low
,You have 50 uL of DNA extract with a DNA concentration of 200 ng per uL.
What is your Total Yield of DNA? - correct answer ✔10,000ng
Your initial DNA extract has a DNA concentration of 900 ng/uL. What volume
of this extract would you add to water to get 50 uL of 100 ng/uL DNA solution.
- correct answer ✔add 5.56 uL of extract to 44.44 uL of water
Your initial DNA extract has a DNA concentration of 10 ng/uL. What volume of
this extract would you add to water to get 50 uL of 100 ng/uL DNA solution. -
correct answer ✔This is not possible
The building blocks of DNA are: - correct answer ✔Nucleotides
Short, single stranded pieces of DNA that are designed to base pair (or match
up with) a specific segment of DNA you want to copy are called: - correct
answer ✔primers
During the annealing step... - correct answer ✔Primers bind to the newly
separated DNA strand
During this step in PCR, DNA is separated into two single strands. - correct
answer ✔Denaturation
What are a necessary "ingredients" for a PCR to proceed - correct answer
✔Nucleotides, DNA polymerase (or Taq polymerase), Reverse Primer,
Template DNA, and Forward Primer
Primer set for plants - correct answer ✔RbcL
, Primer set for Spider - correct answer ✔MT-CO1
Primer set for Octopus (invertebrate) - correct answer ✔MT-CO1
Primer set for mushroom - correct answer ✔ITS
Primer set for Fish - correct answer ✔MT-CO1
Primer set for mouse - correct answer ✔MT-CO1
Primer set for cow - correct answer ✔MT-CO1
According to you lab manual what volume of PCR reactants (if 15uL were in
one) would you add to your master mix if you wanted to amplify 6 samples? -
correct answer ✔105uL
What volume of DNA extract would you add to your master mix before you
distribute it to individual PCR tubes if you wanted to amplify 6 samples? -
correct answer ✔none (does not go in master mix)
This is the volume of master mix that will go into an individual PCR reaction
tube (before any DNA is added) - correct answer ✔28uL
True or False: You should label the tops AND sides of your PCR reaction
tubes - correct answer ✔True
answers verified to pass (34 pgs)
lab week 1-3 - correct answer ✔develop question
lab week 2-5 - correct answer ✔write project proposal
lab week 3-6 - correct answer ✔collect samples for DNA barcoding
lab week 6-10 - correct answer ✔extract dna, pcr, and barcode samples
lab week 10-11 - correct answer ✔write up results
lab week 12-14 - correct answer ✔write discussion and abstract
what is a grant proposal? - correct answer ✔major source of funding for
projects, required proposal in order to receive money
According to your lab manual, the two main factors that contribute to the
quality of your DNA extracts are DNA purity and PCR inhibitors - correct
answer ✔False
True or False: Ethanol, proteins, lipids in a DNA extract can potentially inhibit
PCR reactions (cause them to fail). - correct answer ✔True
This number can indicate the concentration of DNA in your DNA extract -
correct answer ✔A260
,peak wavelength of light DNA absorbs - correct answer ✔260nm
peak wavelength of light proteins absorb - correct answer ✔280nm
peak wavelength of light salts absorb - correct answer ✔230nm
peak wavelength of light carbohydrates absorb - correct answer ✔230nm
ideal DNA concentration (ng/uL) - correct answer ✔100
ideal A260:280 - correct answer ✔1.8
ideal A260:230 - correct answer ✔2.2
A260:230 ratio, below which indicates potentially significant contamination -
correct answer ✔1.4
A260:280 ratio, below which indicates potentially significant contamination -
correct answer ✔1.4
goal DNA concentration for extraction - correct answer ✔50ng/ul-250ng/ul
After running your DNA extract through a nanodrop spectrophotometer you
find that your extract has a DNA concentration of 0.1 ng/uL and your
A260:280 is 1.80, which of the following should you do to increase the
likelihood of your DNA extract PCR amplifying correctly? - correct answer
✔You should do another extraction, your DNA concentration is too low
,You have 50 uL of DNA extract with a DNA concentration of 200 ng per uL.
What is your Total Yield of DNA? - correct answer ✔10,000ng
Your initial DNA extract has a DNA concentration of 900 ng/uL. What volume
of this extract would you add to water to get 50 uL of 100 ng/uL DNA solution.
- correct answer ✔add 5.56 uL of extract to 44.44 uL of water
Your initial DNA extract has a DNA concentration of 10 ng/uL. What volume of
this extract would you add to water to get 50 uL of 100 ng/uL DNA solution. -
correct answer ✔This is not possible
The building blocks of DNA are: - correct answer ✔Nucleotides
Short, single stranded pieces of DNA that are designed to base pair (or match
up with) a specific segment of DNA you want to copy are called: - correct
answer ✔primers
During the annealing step... - correct answer ✔Primers bind to the newly
separated DNA strand
During this step in PCR, DNA is separated into two single strands. - correct
answer ✔Denaturation
What are a necessary "ingredients" for a PCR to proceed - correct answer
✔Nucleotides, DNA polymerase (or Taq polymerase), Reverse Primer,
Template DNA, and Forward Primer
Primer set for plants - correct answer ✔RbcL
, Primer set for Spider - correct answer ✔MT-CO1
Primer set for Octopus (invertebrate) - correct answer ✔MT-CO1
Primer set for mushroom - correct answer ✔ITS
Primer set for Fish - correct answer ✔MT-CO1
Primer set for mouse - correct answer ✔MT-CO1
Primer set for cow - correct answer ✔MT-CO1
According to you lab manual what volume of PCR reactants (if 15uL were in
one) would you add to your master mix if you wanted to amplify 6 samples? -
correct answer ✔105uL
What volume of DNA extract would you add to your master mix before you
distribute it to individual PCR tubes if you wanted to amplify 6 samples? -
correct answer ✔none (does not go in master mix)
This is the volume of master mix that will go into an individual PCR reaction
tube (before any DNA is added) - correct answer ✔28uL
True or False: You should label the tops AND sides of your PCR reaction
tubes - correct answer ✔True
