Gel Electrophoresis Quiz (Questions + Answers) Solved

What would you expect to happen if you left the gel accidentally in the gel electrophoresis chamber for too long? - ️️the DNA strands would not stop they would continue to move through the gel into the buffer In what situation do scientists need to use the PCR reaction? - ️️to amplify a specific sequence or make many copies of a particular fragment What causes the DNA fragments to move within the gel? - ️️The liquid buffer solution helps the electricity flow when an electric current is applied to the solution in the gel. DNA has a negative charge so it attracts the positive electrode gel and moves through Which DNA fragments move faster? Which slower? - ️️Smaller DNA fragments move faster, while longer DNA strands move slower What causes the normally colorless DNA to glow under UV light? - ️️Ethidum Bromide What tool is used to transfer small liquid solutions of DNA from tubes into the gel? - ️️Micro pipette What chemicals and reactants go into the tube before it goes in to the PCR machine? - ️️primer, Dna strand, water, spare nucleotides, enzyme What is the PCR machine called? - ️️Thermal cycler What is the purpose of the RNA primers in the reaction? - ️️the RNA primers mark the ends of the segment they want to study and tell the reaction what DNA fragment to copy Why are the spare nucleotides needed in the reaction? - ️️they are needed to create the copied DNA What would you expect would happen if the scientist forgot to put the DNA copying enzyme in the tube before putting the tube in the machine? - ️️DNA wouldn't be copied Why do scientists use restriction enzymes? - ️️they cut DNA at specific sequencesWhat would scientists use to see their newly cut fragments of DNA? - ️️Gelelectrophoresis look at papers to study cutting stuff - ️️ How can scientists see what is in their reaction tube after the PCR reaction? - ️️the scientists will perform gel electrphoresis What is the purpose of using a marker to ladder in one lane on your gel? - ️️to compare the lengths of the different DNA strands .. unit bp