C190FINAL EXAM WITH
ANSWERS
[Document subtitle]
Abstract
WGU BioChem OA Review
, WGU BioChem OA Review
Autosomal - answer-Males and Females are equally affected
Polymerase Chain Reaction (PCR) - answer-The process of copying DNA in the lab.
What is needed for Polymerase Chain Reaction? - answer-Template DNA, Nucleotides (dNTPS), DNA
Polymerase, and DNA Primers.
What are the three steps of a Polymerase Chain Reaction (PCR)? - answer-Denaturing, Annealing,
Elongating
What is the first step of a Polymerase Chain Reaction? - answer-Denaturing (DNA is heated up to
separate it)
What is Annealing? - answer-Cooling the Polymerase Chain Reaction. Primers stick to the DNA that you
want to copy and ADD DNA Polymerase
What is elongating? - answer-The Polymerase Chain Reaction is heated and DNA Polymerase adds
nucleotides to build a new DNA strand.
What is NOT involved in PCR? - answer-Ligase
What is base excision repair (BER)? - answer-It is used to repair damage to bases caused by harmful
molecules by removing the base that is damaged and replacing it. #1 GLYCOSYLASES see the damaged
DNA. #2 DNA Polymerase puts the right one back #3 DNA Ligase seals it.
What are the 3 steps in base excision repair? - answer-DNA Glycolysase sees the damage and removes
it, DNA polymerase puts the tight base back, DNA ligase seals it back up.
How many nucleotides does base excision repair remove? - answer-1
What is it called when a large section of the nucleotide is removed from the DNA so that DNA
polymerase can try again? (This corrects DNA damage) - answer-Mismatch Repair (MMR). It is the only
one to occur during REPLICATION - DURING THE PROOFREADING
What repairs a base mismatch? - answer-Mismatch Repair (MMR) repairs errors such as G with T instead
of G with C. DNA Polymerase fixes it. This happens during replication and MMR removes a large section
of the nucleotide from the DNA and DNA Polymerase tries again.
What is DNA Transcription? - answer-When DNA polymerase takes an individual nucleotide and matches
them to the parental sequences to ensure a correct pair. (it must bind with RNA primer to work). ie:
DNA Polymerase binds to DNA to make RNA
What is NecleoTIDE repair - answer-CLEANS damage caused by things such as UV rays and repairs it. A
large section of nucleotides are removed, including the damaged portion, along with a few on each side.
It is then replaced by DNA polymerase.