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MCB 2610 Lab Practical 1 Exam Study Guide Solutions

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MCB 2610 Lab Practical 1 Exam Study Guide Solutions gram positive - ANSWER-purple cocci gram negative - ANSWER-pink rods endospore - ANSWER-responses to certain environmental conditions such as depletion of nutrients stages of growth curve: - ANSWER-lag, exponential (log), stationary, death lag phase - ANSWER-occurs when culture is transferred to fresh growth medium exponential phase - ANSWER-growth is maximal and at a steady rate, culture is stable both chemically and physically stationary phase - ANSWER-cell division of new cell growth is matched by equal amount of cell death death phase - ANSWER-caused by accumulation of toxic waste products, cell damage from exposure, of chemicals in the environment absorbance - ANSWER-change in optical density ©EMILLECT 2024/2025 ACADEMIC YEAR. ALL RIGHTS RESERVED FIRST PUBLISH OCTOBER 2024 Page 2/9 generation time - ANSWER-time required by populations to double in number CHNOPS - ANSWER-carbon, hydrogen, nitrogen, oxygen, phosphorus, sulfur liquid media - ANSWER-to grow large number or organisms (nutrient broth) solid media - ANSWER-used to examine colony morphology of organisms and to help isolate pure cultures complex media - ANSWER-chemical composition is unknown synthetic media - ANSWER-made of strict list of inorganic compounds and carbon sources general purpose media - ANSWER-supports growth of wade range of organisms (nutrient broth, nutrient agar, tryptic soy broth) selective media - ANSWER-media that selects for a certain type of microbe by providing optimal conditions for its growth differential media - ANSWER-allow certain bacteria to be differentiated from others, typically by change in color, production of clearing zones exoenzymes - ANSWER-enzymes that are secreted by cells into their environment 3 common exoenzymes - ANSWER-amylase, caseinase, gelatinase ©EMILLECT 2024/2025 ACADEMIC YEAR. ALL RIGHTS RESERVED FIRST PUBLISH OCTOBER 2024 Page 3/9 hydrolysis - ANSWER-breakdown of complex molecules amylase - ANSWER-catalyzes starch hydrolysis caseinase - ANSWER-hydrolyzes milk protein (casein) into peptides and amino acids gelatinase - ANSWER-breaks down gelatin catalase - ANSWER-protects cells from hydrogen peroxide oxidase - ANSWER-indicates presence of cytochrome c amylase test - ANSWER-red growth= positive plain growth = negative caseinase test - ANSWER-clearing around growth = positiv

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©EMILLECT 2024/2025 ACADEMIC YEAR. ALL RIGHTS RESERVED

FIRST PUBLISH OCTOBER 2024




MCB 2610 Lab Practical 1 Exam Study

Guide Solutions


gram positive - ANSWER✔✔-purple cocci


gram negative - ANSWER✔✔-pink rods


endospore - ANSWER✔✔-responses to certain environmental conditions such as depletion of nutrients


stages of growth curve: - ANSWER✔✔-lag, exponential (log), stationary, death


lag phase - ANSWER✔✔-occurs when culture is transferred to fresh growth medium


exponential phase - ANSWER✔✔-growth is maximal and at a steady rate, culture is stable both

chemically and physically


stationary phase - ANSWER✔✔-cell division of new cell growth is matched by equal amount of cell death


death phase - ANSWER✔✔-caused by accumulation of toxic waste products, cell damage from exposure,

of chemicals in the environment


absorbance - ANSWER✔✔-change in optical density



Page 1/9

, ©EMILLECT 2024/2025 ACADEMIC YEAR. ALL RIGHTS RESERVED

FIRST PUBLISH OCTOBER 2024




generation time - ANSWER✔✔-time required by populations to double in number


CHNOPS - ANSWER✔✔-carbon, hydrogen, nitrogen, oxygen, phosphorus, sulfur


liquid media - ANSWER✔✔-to grow large number or organisms (nutrient broth)


solid media - ANSWER✔✔-used to examine colony morphology of organisms and to help isolate pure

cultures


complex media - ANSWER✔✔-chemical composition is unknown


synthetic media - ANSWER✔✔-made of strict list of inorganic compounds and carbon sources


general purpose media - ANSWER✔✔-supports growth of wade range of organisms (nutrient broth,

nutrient agar, tryptic soy broth)


selective media - ANSWER✔✔-media that selects for a certain type of microbe by providing optimal

conditions for its growth


differential media - ANSWER✔✔-allow certain bacteria to be differentiated from others, typically by

change in color, production of clearing zones


exoenzymes - ANSWER✔✔-enzymes that are secreted by cells into their environment


3 common exoenzymes - ANSWER✔✔-amylase, caseinase, gelatinase



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